CAZyme Information: MGYG000001469_02554

Basic Information

• Species: Neobacillus jeddahensis
• Lineage: Bacteria; Firmicutes; Bacilli; Bacillales_B; DSM-18226; Neobacillus; Neobacillus jeddahensis
• CAZyme ID: MGYG000001469_02554
• CAZy Family: GH18
• CAZyme Description: Cell division suppressor protein YneA

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1654	MGYG000001469_25|CGC1	175511.03	4.9073

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001469	4762944	Isolate	not provided	not provided

• Gene Location: Start: 32432; End: 37396 Strand: +

Full Sequence      

MMEISRYELIPGQNDYTIVIYLNPSMEEFSGELGSLQKNRTSLQEKITGLVREKFPYKNI
TAAKVILGTILVSTIYLGPTGNRASAAQTTTNQVLPSNLYDQYTVQSGDSLSVIAKRLNV
STTSIKTVNGLTSDTIYIGQVLKLPYYTYTVVSGDRLSSIATRFNTSSAAIRSYNQLKSD
TIIVGQKLRIPREPVVNPVTTTPPPVVTTTATSTYTVVGGDSLSVIAKKLNTTVDAIRTL
NQLTTDTIFIGQVLKVPQIQTSSGTSTPQAPAVTEPASPPTNTTVTSPAPTNMTYTVVSG
DSLSLIAKKYATTVEEIKVQNNLSSDIIFVGQSLSIPTTQATQVQPEEPVIQDNTTTYTV
VSGDYLSLIAKHFNTTVESIKTTNQLTTDTIYVGQKLVIPAAIADTVAPAVPKLNTTETI
SMKNQAGYTISGTAEANSSVSLTLTNGSNPPVSGTVKADASGRFETKVDVSSLPDGSITV
VATATDLAGNRSTESRLQVTKDTFVADPVIDSTQQVTIDNVKNYTLFGVTDPAVKVEMTV
SDGVNPPLSTTATANEFGEFRSNVDLSLLKDGALTITVRAIDPSGNTSTVRQASIIKDTN
LAAPVIGSTQIVNSQNAGNYSIFGTARPNSTVNLAVSDGIHPDVRTTALVNANGEFQANV
DVRSLLDMPLTITAIQSSAVGSQSQTAKATIIKDATAPGTAILNNNFIISKTNQSSFLLT
GTGESKSQATIKVSDATGKTVEVKEQVNENGEFRIPVDLSSLQDGDVRFDIVLADQAGNS
STVTTKTLSKDTKEPTSLILDSLPTIFSGNMSKYQISGTAEPFVQLEILVADNGTNVTKT
ITTDANGKFTVPFDMSAFKEGDIKVSFLATDSAGNTGQLQPITLVKDSTAPTVALSTLPP
YVNNLTKSQYSVGGTSVDEGAQVQLVISDGVTTITKTATITNGTFTTNFDLSSLKDGPLS
LAVTPTDKAGNTGTAQTYLIQKDTVVANPIISKNGFNLENQQNVYTIMGTAEANATIRAA
ILNAAGIELGSTTATADRTGFYSIKIPVDAAKSAEIQTASVSQTDLAGNSSTAVTANLNS
HTVAAGETLDSIAKRYNTTVDALKALNHLTSDVITPNQLLRLPVTASEVVNLGYLYSGNT
KDYAQLVNQTGSVNTVSPSYFDINTNGTLKLTYSIDTAFITAMHQQGVRVVPFLSNHWDR
NVGRAMLQNKELASQQIADAVARYNLDGVNVDIENVTEADRENYTEFVRLLREKIPLSKE
VSVAVAANPNGWTTGWHGSYDYTNLAKYADYLMIMSYDESYPGGVAGPVASGKWVEKSIQ
YAIKQNVAPDKIVVGIAHYGRYWIEGQSYGGFGISNTQVEDLIAKYHGTVVYDAASQSPK
AVITIKPGDPQTSVGGTVLAPGTYTIWYENEASITQKLALIGKYNLRGVGNWSITQEVKE
VWNSYATSLPSTVPVTSPVYTPPTVPVVQTYTPYIVVAGDSLSVIASRNQTTVSAIKEIN
NLSSDTIYIGQTLKLPVTTTAPADSVTEPVTVPAPAQTVDEPVTTAPAPAVSTVTYTVVA
GDSLSVIAKRYNTTVTAIKDENHLTTDTIAVGQKLAITTSNSPTAPTVSKYTVVSGDSLS
VIAKRYNTTVTALKAANNLTTDSIYIGQILNIPS

Enzyme Prediction     

No EC number prediction in MGYG000001469_02554.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	1156	1440	1.2e-41	0.8277027027027027

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd02874	GH18_CFLE_spore_hydrolase	2.99e-71	1129	1445	2	313	Cortical fragment-lytic enzyme (CFLE) is a peptidoglycan hydrolase involved in bacterial endospore germination. CFLE is expressed as an inactive preprotein (called SleB) in the forespore compartment of sporulating cells. SleB translocates across the forespore inner membrane and is deposited as a mature enzyme in the cortex layer of the spore. As part of a sensory mechanism capable of initiating germination, CFLE degrades a spore-specific peptidoglycan constituent called muramic-acid delta-lactam that comprises the outer cortex. CFLE has a C-terminal glycosyl hydrolase family 18 (GH18) catalytic domain as well as two N-terminal LysM peptidoglycan-binding domains. In addition to SleB, this family includes YaaH, YdhD, and YvbX from Bacillus subtilis.
COG3858	YaaH	1.98e-68	1042	1442	1	415	Spore germination protein YaaH [Cell cycle control, cell division, chromosome partitioning].
PRK06347	PRK06347	1.51e-35	148	399	332	591	1,4-beta-N-acetylmuramoylhydrolase.
pfam00704	Glyco_hydro_18	9.20e-34	1206	1436	82	306	Glycosyl hydrolases family 18.
PRK06347	PRK06347	1.90e-31	78	336	309	591	1,4-beta-N-acetylmuramoylhydrolase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNF29662.1	0.0	2	1652	1	1735
AST91884.1	0.0	1	1516	1	1488
ADU32435.1	9.41e-294	4	1516	2	1328
QOR65000.1	2.85e-283	2	1455	1	1565
AGK53922.1	4.86e-190	686	1454	351	1131

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5JH8_A	2.42e-24	1136	1442	8	307	Crystalstructure of chitinase from Chromobacterium violaceum ATCC 12472 [Chromobacterium violaceum ATCC 12472]
3CZ8_A	5.09e-23	1217	1435	103	309	ChainA, Putative sporulation-specific glycosylase ydhD [Bacillus subtilis subsp. subtilis str. 168],3CZ8_B Chain B, Putative sporulation-specific glycosylase ydhD [Bacillus subtilis subsp. subtilis str. 168]
6XI3_AAA	9.98e-20	437	782	56	404	ChainAAA, Large adhesion protein (Lap) involved in biofilm formation [Marinobacter nauticus ATCC 49840]
4S3K_A	1.66e-18	1132	1442	110	420	ChainA, Spore germination protein YaaH [Priestia megaterium QM B1551]
4S3J_A	8.84e-16	1125	1442	101	418	Crystalstructure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876],4S3J_B Crystal structure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876],4S3J_C Crystal structure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O31682	3.10e-72	1132	1348	4	217	Putative glycosylase YkvQ OS=Bacillus subtilis (strain 168) OX=224308 GN=ykvQ PE=3 SV=1
O05495	8.82e-24	1081	1435	4	401	Putative sporulation-specific glycosylase YdhD OS=Bacillus subtilis (strain 168) OX=224308 GN=ydhD PE=1 SV=2
O32258	1.77e-22	1130	1444	30	338	Uncharacterized glycosylase YvbX OS=Bacillus subtilis (strain 168) OX=224308 GN=yvbX PE=3 SV=1
O07532	1.94e-22	60	399	51	350	Peptidoglycan endopeptidase LytF OS=Bacillus subtilis (strain 168) OX=224308 GN=lytF PE=1 SV=2
O31852	1.31e-18	214	399	28	200	D-gamma-glutamyl-meso-diaminopimelic acid endopeptidase CwlS OS=Bacillus subtilis (strain 168) OX=224308 GN=cwlS PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999884	0.000134	0.000009	0.000001	0.000000	0.000003

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001469_02554.