CAZyme Information: MGYG000001474_00637

Basic Information

• Species: Clostridium_J sp000821305
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium_J; Clostridium_J sp000821305
• CAZyme ID: MGYG000001474_00637
• CAZy Family: CBM50
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2076	MGYG000001474_13|CGC2	224608.43	4.5632

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001474	4325182	Isolate	not provided	not provided

• Gene Location: Start: 261084; End: 267314 Strand: +

Full Sequence      

MYKLSKSKRKLLSLFVAFTMVLGLISTPYKVVLAEGTQEKIIFEEGFTGDKNRIFASEGW
ILNPEAMAGYDSNTNFGVEKPSAKLEKTGMYIESPGFTLEADGTLTFWAKGQNNLANSEL
YVKAFKDGSWDTLDTLSAENGKLTANQVSNATVVIPKQYTKIRLEYKKGTSNVSIDDIKL
VGIDGGSGPVDPPVDPPVDPPVEGNIISIAEARTKTDTVKVKGIITFKEVSGTSSFNYAI
QDGTAGIALRGKDDLKVGDEVTVEGPVGAYNGLVQIQYSSINQNSSNNTLPTPKEVKIAD
IIDNDGGEKYESQRVKFTNVTIGEIVTGGSTTITDNDGKTANIYKIPELTDIVKNNKIDV
IGVCSQFATSGIGGYQIRVASATDIVKSTVVPEVDTKGPELVVVSPGSLTTTDVRQPIKI
SYEDPSGVSMDSLAITFDGTDVKGKCEITDKYIIYNPAEDLSIGKHKVSITLNDTLGNVG
KLEVEIEVKEKVNVDGLNIYFGQLHSHTNISDGEGSIEDAYKYAKESAGVDFLAVTDHSN
SLENDTKASMADGSVSPEWVNAKNTAKKYSDSNFLGVYAYEMTWSNGTGHMNTFNTPGFE
SRNKEGLKNSDAKALENYYNILKEYPASISQWNHPGKTFGDFNDFALYDPQIDNVVNLIE
VGNGEGQVHGSGYFPSYDFYTRALDKGWHVSPTNNQDNHKGKWGNANTARTVVLSDNLTE
EGLYDALRNMRTYATEDENLSIVYTLNDEVMGSMLSDKPSEVKIKVEVKDADAENIGKVS
VIVNGGAVADSKIINANEGIVEFTLPADYSYYYIRVEQEDKDTAVTAPVWVGAVEKAGIS
KSEASTSMPLKGEELTINTSIYNNEDYPMTVKSMEYSIDGNVINTSAEEFEVKSLGTKQY
SFKYTPKAAGSFNVNVKMTAVINGVEKTYTGVVKLKVTDPSVISRVVVDATHFNDYVAGY
YANSMNNFTKIANSDNFDVRIEKNKITDEILKDAQLLIITAPAKKAGNVGGVPYQPQTFS
DEFISTVKRYADNGGTVIVTGIADYQDGIGEFASSTQVNKLLAAIGATTRINNDEVVDDV
TNSGQPYRLKFKNINTDSQYLSGVVSEQEYSFYSGCSVALDKAAVETGKADWLVKGHETT
YSFDSNKNTTGVAVPKGEVVALASEKLASGANVFVAGTIFMSNFEVQASLDNYGDLQYSN
YNIIMNVLDSVKKEVAVTPISEVRNADMGTVFCIEGYVTAGTNEGNAFFDTIYVQDETAG
INIFPVAGIDLQVGQKVRIIGSVSAYEGEKQINQSSIEVLDEVKIMEPTKMTTNAATLEA
NKGLLVSVTGKVMSIGENNSGAMILNDGSGDVRVFLNGYVGSSDGSIEVGKFNSNIKVGD
TVTAIGLASTDPEGPRIRVRDSKEVSLVQSRESEITILHTNDSHGRVKADSSIIGIDTIS
AIKKSMPNSLLVDAGDTLHGLPFATLNKGADIVNLMKLAGYDLMAPGNHDFNYGYERLLE
LATLATEGENGFNIISANVLKDNNPILLPNKIKEVNGVKVGFFGLTSPETTYKTNPNNVK
GLDFADPILTAKQQVEDLKAQGAEVIIALAHIGTDEATEIRSDMVAEAVDGIDVIIDGHS
HSTYPEGYVTNNGTLIASTGQYEGSLGKVSLVVDNETKEVKVKTAALISKDQALAYTPDS
VVTDEIKSIDKEQSAGLLEVVGKNLTLLNGVREDVRSKETNLGNLIQDSMLYETGAQIAI
SNGGNIRANIDVGDITKGEIITVLPFGNYIVTKYLTGAQVKEVLEFAVSKSPAVNPAFAH
VAGIKFSYDESKPEGQRIIEITMDGKKLDMEKKYLVATNDFLAVGGDNYPCFKDIPTENE
FGAMEEALADYIKSLGEVNYEVEGRITKDTFENVANKEAAAAVTDLINALPESVTLKDKD
LVVSARTAYDNLTEAQKALVSEEIVRRLLEAESEIKKLEEAEKDQKAADAVIEMINKLPA
KLNLKDKDLVVKARKAYEALTEAQKELVSKKLVDKLVKAEKDIKKLEEAANKPTKPGLPQ
TGASPVKPLYFGTAILLIGLGALVFKKRNNTDKKAQ

Enzyme Prediction     

No EC number prediction in MGYG000001474_00637.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK09419	PRK09419	1.66e-100	1410	1887	656	1144	multifunctional 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase/5'-nucleotidase.
COG0737	UshA	1.53e-93	1414	1880	26	503	2',3'-cyclic-nucleotide 2'-phosphodiesterase/5'- or 3'-nucleotidase, 5'-nucleotidase family [Nucleotide transport and metabolism, Defense mechanisms].
PRK09558	ushA	3.40e-78	1415	1886	35	548	bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor; Reviewed
cd07408	MPP_SA0022_N	7.78e-68	1415	1655	1	243	Staphylococcus aureus SA0022 and related proteins, N-terminal metallophosphatase domain. SA0022 is an uncharacterized Staphylococcus aureus UshA-like protein with two putative domains, an N-terminal metallophosphatase domain and a C-terminal nucleotidase domain. SA0022 also contains a putative C-terminal cell wall anchor domain. The N-terminal metallophosphatase domain belongs to a large superfamily of distantly related metallophosphatases (MPPs) that includes: Mre11/SbcD-like exonucleases, Dbr1-like RNA lariat debranching enzymes, YfcE-like phosphodiesterases, purple acid phosphatases (PAPs), YbbF-like UDP-2,3-diacylglucosamine hydrolases, and acid sphingomyelinases (ASMases). MPPs are functionally diverse, but all share a conserved domain with an active site consisting of two metal ions (usually manganese, iron, or zinc) coordinated with octahedral geometry by a cage of histidine, aspartate, and asparagine residues. The conserved domain is a double beta-sheet sandwich with a di-metal active site made up of residues located at the C-terminal side of the sheets. This domain is thought to allow for productive metal coordination.
cd00845	MPP_UshA_N_like	1.53e-60	1415	1668	1	253	Escherichia coli UshA-like family, N-terminal metallophosphatase domain. This family includes the bacterial enzyme UshA, and related enzymes including SoxB, CpdB, YhcR, and CD73. All members have a similar domain architecture which includes an N-terminal metallophosphatase domain and a C-terminal nucleotidase domain. The N-terminal metallophosphatase domain belongs to a large superfamily of distantly related metallophosphatases (MPPs) that includes: Mre11/SbcD-like exonucleases, Dbr1-like RNA lariat debranching enzymes, YfcE-like phosphodiesterases, purple acid phosphatases (PAPs), YbbF-like UDP-2,3-diacylglucosamine hydrolases, and acid sphingomyelinases (ASMases). MPPs are functionally diverse, but all share a conserved domain with an active site consisting of two metal ions (usually manganese, iron, or zinc) coordinated with octahedral geometry by a cage of histidine, aspartate, and asparagine residues. The conserved domain is a double beta-sheet sandwich with a di-metal active site made up of residues located at the C-terminal side of the sheets. This domain is thought to allow for productive metal coordination.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QZY54554.1	1.32e-135	1412	1922	31	538
BCJ95849.1	2.06e-133	1417	1887	43	507
AGA59602.1	3.01e-132	1412	1886	32	502
QQY79959.1	1.03e-129	1409	1887	33	509
AOT71215.1	2.96e-128	1409	1915	35	536

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2Z1A_A	1.21e-56	1415	1886	30	528	Crystalstructure of 5'-nucleotidase precursor from Thermus thermophilus HB8 [Thermus thermophilus HB8]
5H7W_A	1.13e-52	1414	1886	4	522	Crystalstructure of 5'-nucleotidase from venom of Naja atra [Naja atra],5H7W_B Crystal structure of 5'-nucleotidase from venom of Naja atra [Naja atra]
7D0V_A	1.72e-51	1414	1886	4	522	ChainA, Snake venom 5'-nucleotidase [Naja atra],7D0V_B Chain B, Snake venom 5'-nucleotidase [Naja atra]
4H2I_A	1.61e-49	1414	1886	3	521	Humanecto-5'-nucleotidase (CD73): crystal form III (closed) in complex with AMPCP [Homo sapiens],6TVX_A Human CD73 (ecto 5'-nucleotidase) in complex with PSB12676 (an AOPCP derivative, compound 9 in paper) in the closed state [Homo sapiens],6TW0_A Human CD73 (ecto 5'-nucleotidase) in complex with PSB12690 (an AOPCP derivative, compound 10 in publication) in the closed state [Homo sapiens],6TWA_A Human CD73 (ecto 5'-nucleotidase) in complex with PSB12646 (an AOPCP derivative, compound 20 in publication) in the closed state [Homo sapiens],6TWF_A Human CD73 (ecto 5'-nucleotidase) in complex with PSB12604 (an AOPCP derivative, compound 21 in publication) in the closed state [Homo sapiens],6YE1_A Chain A, 5'-nucleotidase [Homo sapiens],6YE1_B Chain B, 5'-nucleotidase [Homo sapiens],6YE2_A Chain A, 5'-nucleotidase [Homo sapiens],6YE2_B Chain B, 5'-nucleotidase [Homo sapiens],6Z9B_A Human Ecto-5'-nucleotidase (CD73) in complex with AOPCP derivative A830 (compound 16 in publication) in the closed form (crystal form III) [Homo sapiens]
6S7F_A	1.61e-49	1414	1886	3	521	HumanCD73 (5'-nucleotidase) in complex with PSB12379 (an AOPCP derivative) in the closed state [Homo sapiens],6S7H_A Human CD73 (5'-nucleotidase) in complex with PSB12489 (an AOPCP derivative) in the closed state [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O34313	1.57e-62	1414	1900	668	1187	Trifunctional nucleotide phosphoesterase protein YfkN OS=Bacillus subtilis (strain 168) OX=224308 GN=yfkN PE=1 SV=1
A9BJC1	9.50e-54	1413	1878	22	487	Mannosylglucosyl-3-phosphoglycerate phosphatase OS=Petrotoga mobilis (strain DSM 10674 / SJ95) OX=403833 GN=mggB PE=1 SV=1
A0A2I4HXH5	6.17e-52	1414	1886	4	522	Snake venom 5'-nucleotidase (Fragment) OS=Naja atra OX=8656 PE=1 SV=1
B6EWW8	1.27e-49	1414	1886	43	561	Snake venom 5'-nucleotidase OS=Gloydius brevicaudus OX=259325 PE=2 SV=1
P29240	1.89e-49	1406	1886	23	546	5'-nucleotidase OS=Diplobatis ommata OX=1870830 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000633	0.998395	0.000266	0.000301	0.000189	0.000160

TMHMM  Annotations     

start	end
12	34
2048	2065