CAZyme Information: MGYG000001474_03513

Basic Information

• Species: Clostridium_J sp000821305
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium_J; Clostridium_J sp000821305
• CAZyme ID: MGYG000001474_03513
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
634	MGYG000001474_17|CGC10	73661.88	5.839

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001474	4325182	Isolate	not provided	not provided

• Gene Location: Start: 956776; End: 958680 Strand: -

Full Sequence      

MRGKFLKINLEKEINQFIQGKNYYSYKFMGAHLAEENGVMGYRFTLWAPRCKSISVVGDF
NNWDGTLNTMVKVHEGGIWSIFIEGLEPGEIYKYEITHKDGKKVLKADPYGFYSEKRPNT
ASILVDSCTYPWGDGRWIGKRRRSDILNGPVNIYELHLGSFIKESENGFLNYKEIGQAII
NHVKLMGYTHIEIMPITEYPLDDSWGYQVTGYFSPTSRYGTIEDLKWFIDHCHRSNIGII
LDWVPGHFCKDQHGLFMFDGAQQYEYDDFKMYNNPGWGTANFNLSKTEVRNFLISSAIYW
IKEFHFDGIRIDAVSNMVYLDFGKGPGQWLPNEDGGNVNYGGKEFLRLLNEELHNLNEGI
ITIAEESTALAYVTSKEEQEGLGFDLKWNMGWMNDTLKYVELQEEDKKFNHNLMNFSMMY
AYYEKFMLAISHDEVVYGKKSLFNKMPGDRWQKFAALRCYLTFMYCHPGKKTLFMGSEFG
QFSEWYFKGGLDFHLMDMDEGHKNTLSFTKELNNLYKGEEALWKLDFDPKGFKWIDPDNN
QQRVLSFIRYGDKMEDTLIIVCNFAPITYYDFKIGVPYNCCYKEVFNSDEKRFGGAGEIM
AEVLFSVKDYIHGMEQSITIKVPPLGAVILKVNN

Enzyme Prediction     

No EC number prediction in MGYG000001474_03513.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	182	481	1.2e-138	0.9933554817275747
CBM48	29	113	3.1e-16	0.8552631578947368

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK05402	PRK05402	0.0	17	631	108	722	1,4-alpha-glucan branching protein GlgB.
PRK14705	PRK14705	0.0	19	631	613	1221	glycogen branching enzyme; Provisional
PRK12313	PRK12313	0.0	9	631	7	626	1,4-alpha-glucan branching protein GlgB.
PRK14706	PRK14706	0.0	28	631	26	620	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	16	632	13	628	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AQW22455.1	3.04e-257	7	631	30	655
AOY52490.1	2.85e-255	7	631	30	655
ASY53018.1	5.68e-255	11	631	4	625
QQA12306.1	5.72e-255	7	631	30	655
AMN31466.1	5.72e-255	7	631	30	655

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQW_A	3.08e-208	11	633	131	774	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	4.36e-208	11	633	131	774	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A	6.17e-208	11	633	131	774	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	8.73e-208	11	633	131	774	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR1_A	8.73e-208	11	633	131	774	Crystalstructure of branching enzyme Y500A/D501A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142],5GR6_A Crystal structure of branching enzyme Y500A/D501A double mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q0SWZ1	2.30e-255	7	631	30	655	1,4-alpha-glucan branching enzyme GlgB 1 OS=Clostridium perfringens (strain SM101 / Type A) OX=289380 GN=glgB1 PE=3 SV=1
Q8XPA2	7.55e-254	7	631	30	655	1,4-alpha-glucan branching enzyme GlgB 1 OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=glgB1 PE=3 SV=1
B0TZI5	4.96e-251	11	632	16	638	1,4-alpha-glucan branching enzyme GlgB OS=Francisella philomiragia subsp. philomiragia (strain ATCC 25017 / FSC 153 / O#319-036) OX=484022 GN=glgB PE=3 SV=1
A0Q593	1.15e-249	11	632	16	638	1,4-alpha-glucan branching enzyme GlgB OS=Francisella tularensis subsp. novicida (strain U112) OX=401614 GN=glgB PE=3 SV=1
Q0TQ16	3.17e-249	2	632	23	658	1,4-alpha-glucan branching enzyme GlgB OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000041	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001474_03513.