CAZyme Information: MGYG000001483_00733

Basic Information

• Species: Arachnia massiliensis
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Propionibacteriales; Propionibacteriaceae; Arachnia; Arachnia massiliensis
• CAZyme ID: MGYG000001483_00733
• CAZy Family: GH23
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
275	MGYG000001483_14|CGC5	30088.86	8.9441

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001483	3211248	Isolate	not provided	not provided

• Gene Location: Start: 161887; End: 162714 Strand: -

Full Sequence      

MLKRLLAAASAAVVGLGLAIAAPQAAHAVDVDQYTTPGDHLVNGRYWKTECELYSSTVVR
CSTDIWAPKVVTVQGQHVLHEGWVFNNMTYLPSARGAWKGNPLATNRTWTAEDGRQWRTE
CDTAATGRNGCRTYALTTVVAHDDGKFVSENQWQFNNIVQFTAASTPAVTSIPAAAPPLA
NVPVEKPAVLPVKSQLNLARAATWDRIARCESGNRWNINTGNGYYGGLQFNLATWRSVGG
TDFAAYPHHATREEQITVANRLHAKRGFQPWSCKP

Enzyme Prediction     

No EC number prediction in MGYG000001483_00733.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam06737	Transglycosylas	2.03e-29	200	273	1	74	Transglycosylase-like domain. This family of proteins are very likely to act as transglycosylase enzymes related to pfam00062 and pfam01464. These other families are weakly matched by this family, and include the known active site residues.
cd13925	RPF	6.42e-29	202	273	1	70	core lysozyme-like domain of resuscitation-promoting factor proteins. Resuscitation-promoting factor (RPF) proteins, found in various (G+C)-rich Gram-positive bacteria, act to reactivate cultures from stationary phase. This protein shares elements of the structural core of lysozyme and related proteins. Furthermore, it shares a conserved active site glutamate which is required for activity, and has a polysaccharide binding cleft that corresponds to the peptidoglycan binding cleft of lysozyme. Muralytic activity of Rpf in Micrococcus luteus correlates with resuscitation, supporting a mechanism dependent on cleavage of peptidoglycan by RPF.
cd00442	Lyz-like	3.72e-04	203	261	1	58	lysozyme-like domains. This family contains several members, including soluble lytic transglycosylases (SLT), goose egg-white lysozymes (GEWL), hen egg-white lysozymes (HEWL), chitinases, bacteriophage lambda lysozymes, endolysins, autolysins, chitosanases, and pesticin. Typical members are involved in the hydrolysis of beta-1,4- linked polysaccharides.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QTO37332.1	9.65e-160	1	273	1	279
AQP51326.1	6.47e-43	32	161	1984	2117
AQP52241.1	8.64e-42	34	161	388	520
AQP44644.1	1.69e-41	31	161	915	1045
QTO38746.1	1.64e-40	194	275	301	382

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4CGE_A	4.04e-20	201	271	1	68	ChainA, Resuscitation-promoting Factor Rpfe [Mycobacterium tuberculosis],4CGE_B Chain B, Resuscitation-promoting Factor Rpfe [Mycobacterium tuberculosis],4CGE_C Chain C, Resuscitation-promoting Factor Rpfe [Mycobacterium tuberculosis],4CGE_D Chain D, Resuscitation-promoting Factor Rpfe [Mycobacterium tuberculosis],4CGE_E Chain E, Resuscitation-promoting Factor Rpfe [Mycobacterium tuberculosis],4CGE_F Chain F, Resuscitation-promoting Factor Rpfe [Mycobacterium tuberculosis]
4KL7_A	6.73e-19	204	271	3	70	ChainA, Resuscitation-promoting factor RpfB [Mycobacterium tuberculosis],4KL7_B Chain B, Resuscitation-promoting factor RpfB [Mycobacterium tuberculosis],4KL7_C Chain C, Resuscitation-promoting factor RpfB [Mycobacterium tuberculosis],4KL7_D Chain D, Resuscitation-promoting factor RpfB [Mycobacterium tuberculosis],4KPM_A Chain A, Resuscitation-promoting factor RpfB [Mycobacterium tuberculosis],4KPM_B Chain B, Resuscitation-promoting factor RpfB [Mycobacterium tuberculosis],4KPM_C Chain C, Resuscitation-promoting factor RpfB [Mycobacterium tuberculosis],4KPM_D Chain D, Resuscitation-promoting factor RpfB [Mycobacterium tuberculosis]
4EMN_A	6.91e-19	204	271	4	71	ChainA, Probable resuscitation-promoting factor rpfB [Mycobacterium tuberculosis],4EMN_B Chain B, Probable resuscitation-promoting factor rpfB [Mycobacterium tuberculosis],4EMN_C Chain C, Probable resuscitation-promoting factor rpfB [Mycobacterium tuberculosis],4EMN_D Chain D, Probable resuscitation-promoting factor rpfB [Mycobacterium tuberculosis]
2N5Z_A	9.92e-19	204	271	9	73	Mycobacteriumtuberculosis: a dynamic view of the resuscitation promoting factor RpfC catalytic domain [Mycobacterium tuberculosis H37Rv]
4OW1_A	1.30e-18	204	271	6	70	ChainA, Resuscitation-promoting factor RpfC [Mycobacterium tuberculosis],4OW1_B Chain B, Resuscitation-promoting factor RpfC [Mycobacterium tuberculosis],4OW1_E Chain E, Resuscitation-promoting factor RpfC [Mycobacterium tuberculosis],4OW1_S Chain S, Resuscitation-promoting factor RpfC [Mycobacterium tuberculosis],4OW1_T Chain T, Resuscitation-promoting factor RpfC [Mycobacterium tuberculosis],4OW1_U Chain U, Resuscitation-promoting factor RpfC [Mycobacterium tuberculosis],4OW1_W Chain W, Resuscitation-promoting factor RpfC [Mycobacterium tuberculosis],4OW1_X Chain X, Resuscitation-promoting factor RpfC [Mycobacterium tuberculosis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q6M6W5	2.86e-22	204	271	41	108	Resuscitation-promoting factor Rpf1 OS=Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) OX=196627 GN=rpf1 PE=3 SV=1
O53177	2.66e-19	176	271	79	165	Resuscitation-promoting factor RpfE OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=rpfE PE=1 SV=1
O86308	4.11e-18	162	271	5	111	Resuscitation-promoting factor Rpf OS=Micrococcus luteus OX=1270 GN=rpf PE=1 SV=2
P9WG30	1.37e-17	194	271	34	111	Resuscitation-promoting factor RpfA OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=rpfA PE=3 SV=1
P9WG31	1.37e-17	194	271	34	111	Resuscitation-promoting factor RpfA OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=rpfA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000210	0.999136	0.000157	0.000186	0.000157	0.000139

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001483_00733.