CAZyme Information: MGYG000001507_00796

Basic Information

• Species: Paenibacillus ihuae
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus; Paenibacillus ihuae
• CAZyme ID: MGYG000001507_00796
• CAZy Family: GH66
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1703	MGYG000001507_1|CGC13	186828.11	4.4289

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001507	6631509	Isolate	not provided	not provided

• Gene Location: Start: 835953; End: 841064 Strand: +

Full Sequence      

MVRKQAVLFLCFVLLLVQTSTTGYARSGAAQAEPTGEVNPKILKMVPSKARFAPGEAAEL
NLTLDQSADWSGKLHLQIYQLNTLIAEGSKDLTVLQEGSNMLTVGWTPPAENFRGYTAKA
WIEGAAPTDYVTAAIDVSSDWTHFPRYGYVADFPQETAEESDSKLRELSQDYYINAYQFY
DWMWRHDVSVYSKTDENGQPLTDADGNFIDEDINTDTHYTDLLGRSLYPLSVKQQVEAAQ
KYGSAAMAYQMNYAARENYEDFGVSPEWGLYNSAQLDINNPQKDQNGYTFDVNGKTTSLF
LQDPGNKFWQDYITRQFDRSINTFGFDGIHLDQWGANDNNYLYDYNGNKRYYSLDYAQLI
NSVKDSLLVNNPDKSKVTFNMVGGNAEYSAVPDPDTKTDFDYSEIWQDRNHYRDIQKVVE
DTREKDGGKAMVIAGYMNYKEATGLPTRGTEALDVPVTLDYQSRIAKAYGWVGNFGRKDT
DAVTFTVNAPADGLYTLVLNYGQGNGSGSPEGKLTVNNEIAAAAIPFEANTGWGNPTAQA
TVAATLKRGENSVKLTLNTNNLWLNLASLEVQGQGIKERYEAVDAQLDTVIVDQYSHVYY
FDTAGDYVKFQVNVPAEGDYPLGFSYASDWQDVRRELFVNNVPQGELTFAGMGTWDKFSE
LNNLQTVHLEEGDNTITLKASTNDLGIKLQYMTAGTQRYYAMYADIPQTNSMNYSESKTD
NFGQAGQTVTYNVYADHPLDSITVLYHGDNDPVMSVLVDGEPAPQAQNITFPKTAGGWSG
TMQPLELSVPLQEGYHKVTLKMESSGQYTNVGGIIAGGYEYSTGSAEVTGGVVPLTGYAF
DFNNVNDRVAFTVNIGQSGLYDLGWIYQNDAQGAVTVSRSVYINNGAGIPISFAPTTGSG
WEEAAVNGIRLAEGDNTIIIKMPEGQDSGIRLDKLNVTLSGAPDAVTRSYEAESTNTESP
FSLYKDTVLNFGEVGQQVTYPVNIPQSGEQSLIFTYSNPGGLTTRSVYIDGVRAKDEHGK
ELKIGLDGTESIEKYSGDGYVIIPHMEAGIHTVTLRMEADDIAGTIRLRGVTAGYFNEPS
VRLMDAGLASMGATHIELGTAEQQSEGPNMLAHEYYPNRSKKMLASTKVTMQEYYKFNAA
YENLLFDSKADSSAMVSVEADGSKLAISKDGMDNTLWATVRKNDANPGFERYDVLHLINL
LNNDGNWRNAANEPAVLHSLKVSYDIGITEKEAPDLKVYAATPDAGHGLSQELEYSWDGD
KLLIELPSLQYWTMIYIDKDPETVMVQPIFSSEEPGLPTQAPVVTAAPTPAATAAPGLLG
STAGNSATPTPAAAKREALVLQAKDLSVAANGNISIAVNEEKYNRMLIPVSAVEGLQGEN
LLLQGDMFTLHFMQDHILQLLEANKGKLTADSNIQITFDKIEPKDASLPDGKPWISAGPV
YEFEFGVTTANEGVSRLDSFRSPVQLTLKLDDLAGQGELNDLLGIYSFLEPLRKWEYVSS
RADLAAQQISADLYHFSRYTIFAYDKSFTDVPAEHWAFKAIHKLTAKHVISGMTENTFVP
LAKITRAQFAALLVRALQLKADRPVPFKDVAADSWYAEDIAAAYQAGLITGRSGERFAPN
ERITRQEMAVMLAGAFIKAGGTDRSAESSFAFRDSNEISAWAQAAVQIVQSAGLMKGYAD
GAFVPGANATRAEAAQAIAYLLK

Enzyme Prediction     

EC	3.2.1.11	2.4.1.248

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH66	45	442	7.7e-111	0.6438848920863309
CBM35	580	689	5.7e-22	0.9495798319327731
CBM35	457	571	5.5e-18	0.907563025210084

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd14745	GH66	4.78e-90	143	454	1	275	Glycoside Hydrolase Family 66. Glycoside Hydrolase Family 66 contains proteins characterized as cycloisomaltooligosaccharide glucanotransferase (CITase) and dextranases from a variety of bacteria. CITase cyclizes part of a (1-6)-alpha-D-glucan (dextrans) chain by formation of a (1-6)-alpha-D-glucosidic bond. Dextranases catalyze the endohydrolysis of (1-6)-alpha-D-glucosidic linkages in dextran. Some members contain Carbohydrate Binding Module 35 (CBM35) domains, either C-terminal or inserted in the domain or both.
pfam13199	Glyco_hydro_66	1.64e-85	49	452	3	368	Glycosyl hydrolase family 66. This family is a set of glycosyl hydrolase enzymes including cycloisomaltooligosaccharide glucanotransferase (EC:2.4.1.-) and dextranase (EC:3.2.1.11) activities.
pfam13199	Glyco_hydro_66	1.46e-39	1070	1276	358	557	Glycosyl hydrolase family 66. This family is a set of glycosyl hydrolase enzymes including cycloisomaltooligosaccharide glucanotransferase (EC:2.4.1.-) and dextranase (EC:3.2.1.11) activities.
cd14745	GH66	1.09e-18	1070	1141	266	331	Glycoside Hydrolase Family 66. Glycoside Hydrolase Family 66 contains proteins characterized as cycloisomaltooligosaccharide glucanotransferase (CITase) and dextranases from a variety of bacteria. CITase cyclizes part of a (1-6)-alpha-D-glucan (dextrans) chain by formation of a (1-6)-alpha-D-glucosidic bond. Dextranases catalyze the endohydrolysis of (1-6)-alpha-D-glucosidic linkages in dextran. Some members contain Carbohydrate Binding Module 35 (CBM35) domains, either C-terminal or inserted in the domain or both.
NF033190	inl_like_NEAT_1	2.10e-15	1528	1696	582	746	NEAT domain-containing leucine-rich repeat protein. Members of this family have an N-terminal NEAT (near transporter) domain often associated with iron transport, followed by a leucine-rich repeat region with significant sequence similarity to the internalins of Listeria monocytogenes. However, since Bacillus cereus (from which this protein was described, in PMID:16978259) is not considered an intracellular pathogen, and the function may be iron transport rather than internalization, applying the name "internalin" to this family probably would be misleading.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QSF46225.1	0.0	1	1703	1	1703
AIQ39879.1	0.0	1	1703	1	1701
AEX15594.1	0.0	29	1702	38	1692
AIQ34445.1	0.0	39	1702	46	1701
BCG57719.1	0.0	1	1702	1	1980

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3WNK_A	5.98e-84	42	569	27	526	ChainA, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans]
3WNP_A	2.12e-83	42	569	8	507	ChainA, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans],3WNP_B Chain B, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans]
3WNL_A	5.25e-83	42	569	8	507	ChainA, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans],3WNM_A Chain A, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans],3WNN_A Chain A, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans],3WNN_B Chain B, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans],3WNO_A Chain A, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans],3WNO_B Chain B, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans]
5X7G_A	2.48e-78	44	569	28	525	CrystalStructure of Paenibacillus sp. 598K cycloisomaltooligosaccharide glucanotransferase [Paenibacillus sp. 598K],5X7H_A Crystal Structure of Paenibacillus sp. 598K cycloisomaltooligosaccharide glucanotransferase complexed with cycloisomaltoheptaose [Paenibacillus sp. 598K]
5AXG_A	2.10e-46	49	451	51	419	Crystalstructure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus [Thermoanaerobacter pseudethanolicus ATCC 33223],5AXG_B Crystal structure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus [Thermoanaerobacter pseudethanolicus ATCC 33223]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P94286	2.17e-84	3	937	9	870	Cycloisomaltooligosaccharide glucanotransferase OS=Niallia circulans OX=1397 PE=1 SV=1
P70873	1.58e-77	4	937	2	862	Cycloisomaltooligosaccharide glucanotransferase OS=Niallia circulans OX=1397 GN=cit PE=3 SV=1
P38536	1.57e-35	1324	1703	1502	1856	Amylopullulanase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=amyB PE=3 SV=2
P19424	1.19e-25	1528	1702	41	214	Endoglucanase OS=Bacillus sp. (strain KSM-635) OX=1415 PE=1 SV=1
C6CRV0	2.25e-21	1499	1695	1254	1452	Endo-1,4-beta-xylanase A OS=Paenibacillus sp. (strain JDR-2) OX=324057 GN=xynA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000235	0.999159	0.000145	0.000169	0.000141	0.000135

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001507_00796.