CAZyme Information: MGYG000001514_03613

Basic Information

• Species: Paenibacillus_A ihumii
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus_A; Paenibacillus_A ihumii
• CAZyme ID: MGYG000001514_03613
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1150	MGYG000001514_12|CGC38	130267.3	4.9562

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001514	5923787	Isolate	not provided	not provided

• Gene Location: Start: 2266883; End: 2270335 Strand: -

Full Sequence      

MNKKILFNDRWEFAKSGLETANYTDLPFEPVDIPHDWLIYNTLNLYEDSIGWYRKKFRWT
KNDKQLLLCFDGIYMDSSVYVNGQFVGEWKYGYSSFELEITGAVTEGENEILVKVVHQSP
NSRWYSGAGIYRNVWLKERACNYIVTDGVYISTKQMDQGWEIEIDTELSLHEDVYVSHIL
LDQGRVIAEVTDKITAVAGAVTISRQNLMVQMPRLWSPDEPYLYQLITRLRSDVADQKEN
PQADTDHMIETISHPIGFRNIRLDPTQGFFLNGKKMKLNGVCEHHDLGALGAAFNKAALR
RRFSILKDMGVNAVRTAHNMPAKELMDLADEMGLLVVSEAFDMWERPKTPYDYARFFKDW
AHRDVRSWVMRDRNHPSLIMWSIGNEIYDTHADERGQEITRMLMDAVLEYDPKQNARVTI
GSNYMPWENAQQCADIVKVAGYNYAEKYYEQHHQEHPDWIIYGSETASVVQSRGIYHFPF
GKSILADDDEQCSALGNSSTSWGAKSAEACILADRDTPFSLGQFIWTGFDYIGEPTPYHT
KNSYFGQIDTATFPKDSYYIYQSAWTDYRTRPMVHILPYWDFNPGQMIDVRVCSNAPRIE
LLFNGQTIGTHDIDHEHGAELVGWWTIPYAPGELKAIAYDETGRIIATDVKTSFGDARKI
SLKADKEQLLADGQDLIFVEIAMEDEAGRPVGNANNRVHVEVTGAGRLIGLDNGDSTDYD
PYKGQSRRLFSGKLMAIIGATTQSGEIRMQVTSRGLAGQSARFMSIPAGAGTVSAGICAR
TENKELPCVMGAPDEVPLRKIELISEAGQKFDPSRREMVVRAQLHPPETSYRDVEWSVVN
DAGIESNIAKVEADGLEARITALGDGEFRLRCMSRNGTDKIKLISQLEFAASGLGAAYKD
PYKFISAGLYDYSKGELGNGNERGVATSRDGETQVGFRDIDFGPYGSDTITIPIFALSSE
PYPLEIWEGMPGEEGSQLLADAIYQKETRWNVYQEETYRLSKPLRGVTSLCFVLRQKVHI
KGFYFEPRNRAFERNSAAHSDRIYGDTYTVADNRVEGIGNNVSLVFEQMDFSSEGATRLV
IYGRSPIDKNTIHVRFGSEEGESNQLVEFTQSDGYEERVFDIKKITGQGKVTFIFLPGSQ
FDFGWFRFER

Enzyme Prediction     

EC	3.2.1.8	3.1.1.73

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	2	629	6.2e-112	0.6954787234042553

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.68e-64	23	581	62	614	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	3.84e-44	48	449	62	473	beta-D-glucuronidase; Provisional
pfam18565	Glyco_hydro2_C5	9.02e-39	660	762	1	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10340	ebgA	1.49e-38	22	386	89	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	3.82e-27	47	386	118	462	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AZK49101.1	0.0	1	1150	1	1149
AVV58790.1	0.0	1	1150	1	1152
ANA82470.1	0.0	1	1150	1	1152
AWP27978.1	0.0	1	1150	1	1152
AYB44754.1	0.0	1	1150	1	1152

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4YPJ_A	3.08e-179	3	766	8	808	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
7CWD_A	6.99e-175	3	756	2	792	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
5T98_A	2.82e-152	5	756	28	811	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
4CU6_A	8.42e-152	2	753	13	832	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	2.31e-151	2	753	13	832	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	1.35e-161	7	756	47	839	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	5.30e-96	7	765	51	818	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
P77989	1.16e-46	1	760	1	732	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KPJ7	9.97e-46	8	677	57	745	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KN75	6.73e-44	7	837	31	872	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999605	0.000326	0.000046	0.000003	0.000001	0.000035

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001514_03613.