CAZyme Information: MGYG000001523_01207

Basic Information

• Species: Gracilibacillus massiliensis
• Lineage: Bacteria; Firmicutes; Bacilli; Bacillales_D; Amphibacillaceae; Gracilibacillus; Gracilibacillus massiliensis
• CAZyme ID: MGYG000001523_01207
• CAZy Family: GT2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
687		80751.78	4.8274

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001523	4207224	Isolate	not provided	not provided

• Gene Location: Start: 483122; End: 485185 Strand: +

Full Sequence      

MHKENSYLISICMMVKNEESNLSRCLQSITPLLDRSDVELIIVDTGSIDNTIEIANKYTK
KIFYRKWQDNFSEIRNYSISAANGKWVFIIDADEEFIELDKIIKLLNSNDIDNYNAIQLL
TNNILYKDGSKTIITPSCRIFKNGNLSYDGAIHNQPIFNGPVYNTNIYFNHYGYINDDRE
LMDNKFNRTATILKREILKDPNNVYYQYQLAKSLGMHGDHIEALQQIRTAYKVMKENKQI
KLYIYLTYANLALLNKCYNEAVEISEEGLQFNDQYIDLFFILSKGLYNIKMYHEAIHAYR
RYLKLYEIGENLSIFDDASLEVIYFEENYKLNVIKELIMTAIEINEFKIANEYIHLVNNT
KAKNKLAIELYLKSEDYTALKEYLIVHFHETEYPTEIENLVEEVLNNKDDKVIRDISAIF
ADEETSYGMLNNIRSMDDERVQSILIKEFFQSYDITTLGKNYYVIFEIAISVNFPILKEL
KKASSRYIKHLVLYSFEKSTDLSSYFYDYMYSTSNIRTSDFQTNRVYCAISNPILLHEQE
KANKEENKQLKDERLFEAFNQYIASGENYINLLYQVDRLRINYSVVDDMEHQLFILLYLS
AQSAKKGDVKTTISYYKKAVDVYKPFAKYLEIYLGKLYQDIAKGLIEKNMYEEAMEMYEE
ALIKSESDEEQDDILNKITSLEGSQQP

Enzyme Prediction     

No EC number prediction in MGYG000001523_01207.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	10	109	3.9e-17	0.6235294117647059

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd02511	Beta4Glucosyltransferase	8.19e-41	9	193	2	183	UDP-glucose LOS-beta-1,4 glucosyltransferase is required for biosynthesis of lipooligosaccharide. UDP-glucose: lipooligosaccharide (LOS) beta-1-4-glucosyltransferase catalyzes the addition of the first residue, glucose, of the lacto-N-neotetrase structure to HepI of the LOS inner core. LOS is the major constituent of the outer leaflet of the outer membrane of gram-positive bacteria. It consists of a short oligosaccharide chain of variable composition (alpha chain) attached to a branched inner core which is lined in turn to lipid A. Beta 1,4 glucosyltransferase is required to attach the alpha chain to the inner core.
pfam00535	Glycos_transf_2	6.67e-15	10	112	1	109	Glycosyl transferase family 2. Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.
cd02525	Succinoglycan_BP_ExoA	6.44e-12	8	208	1	208	ExoA is involved in the biosynthesis of succinoglycan. Succinoglycan Biosynthesis Protein ExoA catalyzes the formation of a beta-1,3 linkage of the second sugar (glucose) of the succinoglycan with the galactose on the lipid carrie. Succinoglycan is an acidic exopolysaccharide that is important for invasion of the nodules. Succinoglycan is a high-molecular-weight polymer composed of repeating octasaccharide units. These units are synthesized on membrane-bound isoprenoid lipid carriers, beginning with galactose followed by seven glucose molecules, and modified by the addition of acetate, succinate, and pyruvate. ExoA is a membrane protein with a transmembrance domain at c-terminus.
cd00761	Glyco_tranf_GTA_type	7.14e-12	11	127	1	120	Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold. Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.
TIGR04195	S_glycosyl_SunS	1.40e-11	9	366	59	407	peptide S-glycosyltransferase, SunS family. Members of this family include SunS, the S-glycosyltransferase that transfers a sugar (substrate is variable in reconstitution assays) onto the precursor of the glycopeptide sublancin, which once was thought to be a lantibiotic.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALS24476.1	3.03e-131	9	634	7	631
AIY81238.1	6.74e-117	9	642	3	634
AJF28809.1	6.74e-117	9	642	3	634
ACD53692.1	6.74e-117	9	642	3	634
AJF31870.1	6.74e-117	9	642	3	634

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7MSK_A	1.36e-13	9	365	68	414	ChainA, Glyco_trans_2-like domain-containing protein [Bacillus thuringiensis serovar andalousiensis BGSC 4AW1],7MSK_B Chain B, Glyco_trans_2-like domain-containing protein [Bacillus thuringiensis serovar andalousiensis BGSC 4AW1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P42460	1.54e-15	9	97	10	94	TPR repeat-containing protein Synpcc7942_0270 OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=Synpcc7942_0270 PE=4 SV=1
Q92IX8	1.14e-08	1	162	1	166	Uncharacterized glycosyltransferase RC0292 OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=RC0292 PE=3 SV=1
Q68XF1	1.25e-08	9	214	4	216	Uncharacterized glycosyltransferase RT0209 OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=RT0209 PE=3 SV=1
P44029	9.00e-08	9	113	4	105	Uncharacterized glycosyltransferase HI_0653 OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=HI_0653 PE=3 SV=1
O05944	9.54e-08	9	199	4	202	Uncharacterized glycosyltransferase RP128 OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=RP218 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000025	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001523_01207.