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CAZyme Information: MGYG000001525_03354
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Basic Information |
Genomic context |
Full Sequence |
Enzyme annotations |
CAZy signature domains |
CDD domains |
CAZyme hits |
PDB hits |
Swiss-Prot hits |
SignalP and Lipop annotations |
TMHMM annotations
Basic Information help
| Species | Paenibacillus_A rubinfantis | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Lineage | Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus_A; Paenibacillus_A rubinfantis | |||||||||||
| CAZyme ID | MGYG000001525_03354 | |||||||||||
| CAZy Family | GH32 | |||||||||||
| CAZyme Description | hypothetical protein | |||||||||||
| CAZyme Property |
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| Genome Property |
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| Gene Location | Start: 1133847; End: 1136126 Strand: - | |||||||||||
Full Sequence Download help
| MSQLTGEQGL MMHWAFDEGA GRSALEQVSQ SRDEIQYVLN QAEFTEPVDP QWRQGVVGSG | 60 |
| LLFDGYSTSI ARPASELDRN RGAEPLSALS IGVWVAPRSY DWGYEGKLAA IVNRHNRECE | 120 |
| QGYLLGMFRH GRWSFQVGLD QGGWKEVWSP DGDELPKHEW SYVNAVFNGE QGVIKLFLNG | 180 |
| REIASSEVPV GSRMVEAVGT DLLIGKNNHS SRWAGVFHLQ MFSGILDELK IYRRALSGEE | 240 |
| IAASYRQVLD SLYGGIIPQV PYDEIKLDRT PLLLDRHRPQ YHASPPAHWM NEPHAPIYFE | 300 |
| GQYHLFYQHN PLGPYFYQIH WGHWVSEDLV HWRDLPVALA PEKDQLAPDG IWSGSATYDA | 360 |
| EGLPVLFFTA GNDSASPNQS VALARSTYPA DGNPDLVRWV KHREPLIVQQ KGIGAFGDFR | 420 |
| DPFVWKDDDG WYALVGSGIE GEGGAALAFV SKDMLNWTYK GPFFQADLQK YPYLGPIWEL | 480 |
| PVLLPLGTDK QGENKHLLLV SPVGEGADVE VFYWIGRLDK EELSFIPDQE EPELIDVGDF | 540 |
| HFTGPSGMVD PKTGRKIIFT IAQGDRTSEL EYQSGWAHNA GLPLSVYLRD DGRLGIEPIQ | 600 |
| ELQSLRGEKR LSLRDNSLAE VNERLQEVQG DMLEIQLEIE PDSAEQFGIK LRRTPDGEEE | 660 |
| TLLYYDSKQS MLLVDRTKTT LHPGERCRGI QGGKLELAGE NLRLHLYLDR SMVEAYANGL | 720 |
| KSLTTRVYPS RRDALGLEIW GDGELVVRSM EVWEMKSIW | 759 |
CAZyme Signature Domains help
| Family | Start | End | Evalue | family coverage |
|---|---|---|---|---|
| GH32 | 282 | 594 | 6.2e-71 | 0.9829351535836177 |
CDD Domains download full data without filtering help
| Cdd ID | Domain | E-Value | qStart | qEnd | sStart | sEnd | Domain Description |
|---|---|---|---|---|---|---|---|
| smart00640 | Glyco_32 | 9.02e-110 | 282 | 719 | 1 | 436 | Glycosyl hydrolases family 32. |
| cd08996 | GH32_FFase | 1.88e-107 | 288 | 588 | 1 | 281 | Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. |
| COG1621 | SacC | 8.10e-82 | 277 | 757 | 28 | 486 | Sucrose-6-phosphate hydrolase SacC, GH32 family [Carbohydrate transport and metabolism]. |
| TIGR01322 | scrB_fam | 4.39e-76 | 275 | 730 | 11 | 445 | sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides] |
| pfam00251 | Glyco_hydro_32N | 1.19e-68 | 282 | 592 | 1 | 301 | Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. |
CAZyme Hits help
| Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End |
|---|---|---|---|---|---|
| AZS14151.1 | 0.0 | 1 | 759 | 1 | 759 |
| QQZ63908.1 | 0.0 | 1 | 759 | 1 | 759 |
| QLG40432.1 | 0.0 | 1 | 759 | 1 | 759 |
| AIQ60039.1 | 0.0 | 1 | 759 | 1 | 760 |
| CQR54044.1 | 0.0 | 1 | 759 | 1 | 759 |
PDB Hits download full data without filtering help
| Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
|---|---|---|---|---|---|---|
| 7VCO_A | 1.25e-55 | 276 | 758 | 24 | 488 | ChainA, Sucrose-6-phosphate hydrolase [Frischella perrara],7VCP_A Chain A, Sucrose-6-phosphate hydrolase [Frischella perrara] |
| 3PIG_A | 3.24e-41 | 275 | 758 | 37 | 515 | beta-fructofuranosidasefrom Bifidobacterium longum [Bifidobacterium longum],3PIG_B beta-fructofuranosidase from Bifidobacterium longum [Bifidobacterium longum],3PIJ_A beta-fructofuranosidase from Bifidobacterium longum - complex with fructose [Bifidobacterium longum],3PIJ_B beta-fructofuranosidase from Bifidobacterium longum - complex with fructose [Bifidobacterium longum] |
| 6NUM_A | 6.75e-39 | 275 | 758 | 37 | 515 | Thestructure of GH32 from Bifidobacteium adolescentis [Bifidobacterium adolescentis],6NUN_A Structure of GH32 hydrolase from Bifidobacterium adolescentis in complex with frutose [Bifidobacterium adolescentis] |
| 1UYP_A | 7.31e-37 | 278 | 759 | 3 | 431 | Thethree-dimensional structure of beta-fructosidase (invertase) from Thermotoga maritima [Thermotoga maritima MSB8],1UYP_B The three-dimensional structure of beta-fructosidase (invertase) from Thermotoga maritima [Thermotoga maritima MSB8],1UYP_C The three-dimensional structure of beta-fructosidase (invertase) from Thermotoga maritima [Thermotoga maritima MSB8],1UYP_D The three-dimensional structure of beta-fructosidase (invertase) from Thermotoga maritima [Thermotoga maritima MSB8],1UYP_E The three-dimensional structure of beta-fructosidase (invertase) from Thermotoga maritima [Thermotoga maritima MSB8],1UYP_F The three-dimensional structure of beta-fructosidase (invertase) from Thermotoga maritima [Thermotoga maritima MSB8] |
| 1W2T_A | 1.82e-36 | 278 | 759 | 3 | 431 | beta-fructosidasefrom Thermotoga maritima in complex with raffinose [Thermotoga maritima MSB8],1W2T_B beta-fructosidase from Thermotoga maritima in complex with raffinose [Thermotoga maritima MSB8],1W2T_C beta-fructosidase from Thermotoga maritima in complex with raffinose [Thermotoga maritima MSB8],1W2T_D beta-fructosidase from Thermotoga maritima in complex with raffinose [Thermotoga maritima MSB8],1W2T_E beta-fructosidase from Thermotoga maritima in complex with raffinose [Thermotoga maritima MSB8],1W2T_F beta-fructosidase from Thermotoga maritima in complex with raffinose [Thermotoga maritima MSB8] |
Swiss-Prot Hits download full data without filtering help
| Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
|---|---|---|---|---|---|---|
| P0DJA7 | 7.24e-50 | 272 | 757 | 23 | 501 | Sucrose-6-phosphate hydrolase OS=Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) OX=264203 GN=sacA PE=1 SV=1 |
| F8DVG5 | 9.92e-50 | 272 | 757 | 23 | 501 | Sucrose-6-phosphate hydrolase OS=Zymomonas mobilis subsp. mobilis (strain ATCC 10988 / DSM 424 / LMG 404 / NCIMB 8938 / NRRL B-806 / ZM1) OX=555217 GN=sacA PE=3 SV=1 |
| Q01IS7 | 9.98e-46 | 260 | 757 | 28 | 591 | Beta-fructofuranosidase, insoluble isoenzyme 2 OS=Oryza sativa subsp. indica OX=39946 GN=CIN2 PE=2 SV=2 |
| Q0JDC5 | 9.98e-46 | 260 | 757 | 28 | 591 | Beta-fructofuranosidase, insoluble isoenzyme 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CIN2 PE=1 SV=1 |
| B6DZD0 | 1.14e-44 | 259 | 757 | 46 | 585 | Fructan 1-exohydrolase OS=Triticum urartu OX=4572 GN=1-FEH PE=3 SV=1 |
SignalP and Lipop Annotations help
This protein is predicted as OTHER
| Other | SP_Sec_SPI | LIPO_Sec_SPII | TAT_Tat_SPI | TATLIP_Sec_SPII | PILIN_Sec_SPIII |
|---|---|---|---|---|---|
| 1.000017 | 0.000020 | 0.000001 | 0.000000 | 0.000000 | 0.000000 |