CAZyme Information: MGYG000001525_04799

Basic Information

• Species: Paenibacillus_A rubinfantis
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus_A; Paenibacillus_A rubinfantis
• CAZyme ID: MGYG000001525_04799
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
831		95926.85	4.7013

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001525	5369528	Isolate	not provided	not provided

• Gene Location: Start: 1386983; End: 1389478 Strand: -

Full Sequence      

MRLVDLGGAWRMKRLDQTEWLPGTVPGSVYYDLLNAGQMPDPYYRDQEYEVLELSKYDYE
YERIFQVDESTLAHDQVVLLCEGLDTLAEVYLNGTLILNADNMHRTYEVDIKSLLKLGEN
HIHVILRSPVEYVLRKQAERPLIGCADAVPGISHLRKAHSMFGWDWGPQLPDLGIWRDMT
IRGYDRSRIEDVYITQTHEPDKVNLDVRVRTDSWAEGNREIAVRVTRPSGEGLVTQVSEQ
IEPTTTGSVGALQELHIPVEIPRPELWWPNGLGEQPLYEVEIVLSEQGRELDRHDLRIGL
RTLTVLQEEDEWGESFQLVVNGIPFFSMGADYIPEDNLFPRRSRERTEHLIRSCAEANFN
TIRVWGGGHYPEDYFYDLCDEYGIVVWQDHMYACGVYELTEDFKTNITQETIDNMKRLRH
HASLGLWCGNNEQELAWDEWNWAEQTSLQLQADYIKMYEVLLPEIAKEIDPNTFYWRASP
SSKGSFDKPNDENYGDMHYWDVWHGKKPFTAYRTLYPRYMSEFGLQSFPSLKTVETFTLP
EDRNIFSYVMESHQKNGTGNEKILYYIGETYRYPKDFSSLLYASQLIQAEGMRCGVEHWR
RNRGRCMGALYWQLNDIWPVASWSSIDYYGRWKALQYEAKRFFAPVLASACEEGTQVALH
VSNESKRTVKGKLVWSLRNARSEVLRQGEQAGEVAPFTSVELEQLDFAEELGTKAKLRKT
YLEYAWVEDGEIVSGGTVLFVKAKHFGFANPNIQAVITEAEDRFTITLTSEAYARFVELD
FTGLDAIFSDNYFDLSGGARKTVTIAKSALSRPTALAELQEQFTVRSVFDI

Enzyme Prediction     

No EC number prediction in MGYG000001525_04799.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	5	755	1e-105	0.761968085106383

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.20e-104	5	781	14	763	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam17753	Ig_mannosidase	3.90e-26	750	830	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
pfam17786	Mannosidase_ig	2.93e-15	655	745	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
PRK10340	ebgA	3.45e-15	61	432	113	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam00703	Glyco_hydro_2	3.58e-14	188	301	2	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AIQ37152.1	0.0	1	831	1	821
AIQ75814.1	0.0	1	831	1	821
AIQ25428.1	0.0	1	831	1	821
QLG40119.1	0.0	1	831	1	821
ANS75587.1	0.0	1	830	1	819

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6BYE_A	3.03e-182	1	828	3	850	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306],6BYE_B Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306]
6BYC_A	3.12e-182	1	828	3	850	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
6BYI_A	2.25e-181	1	828	1	848	Crystalstructure of the acid-base mutant (E477A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306],6BYI_B Crystal structure of the acid-base mutant (E477A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
6BYG_A	2.32e-181	1	828	3	850	Crystalstructure of the nucleophile mutant (E575A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306],6BYG_B Crystal structure of the nucleophile mutant (E575A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
2VJX_A	1.70e-180	3	823	10	838	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A2QYN2	1.22e-116	6	805	9	828	Beta-mannosidase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=mndB PE=3 SV=1
Q4WAH4	3.58e-115	1	653	4	663	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
B0YBU9	1.37e-114	1	653	4	663	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
A1D911	1.37e-114	1	653	4	663	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
Q29444	2.15e-114	4	830	23	877	Beta-mannosidase OS=Bos taurus OX=9913 GN=MANBA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000038	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001525_04799.