CAZyme Information: MGYG000001535_00970

Basic Information

• Species: Paenibacillus_A phocaensis
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus_A; Paenibacillus_A phocaensis
• CAZyme ID: MGYG000001535_00970
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
589	MGYG000001535_9|CGC7	69040.85	4.8842

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001535	5515101	Isolate	not provided	not provided

• Gene Location: Start: 361462; End: 363231 Strand: -

Full Sequence      

MTEPRSEYPRPQFVRSSWRSLNGSWSFAFDDRNLGVKEKWHQGSAEFPMEIRVPFCYQSE
LSGIEDKGVHERVWYEKTFTIPDEYAGKRVILHFGAVDYECRVWVNGMPAGNHRGGFSSF
SFEISDLIAGKDNRITVMARDDLQDLTIPRGKQYWKAKGEVMWFTNMTGIWQSVWLEFVD
DVYLEKVRFTPHLDTNEIEIQTFIDGWDERYRIELDTRIEFAGEVIAEERRRLLAGTESW
RVNLRDFNDHGLGRWWSPEKPNLYDVHFELKVDGQTVDSVDSYFGMRKISVDGGKLCLNN
RPYKMKLVLDQGYFPQSLLTAPTEEALRRDVELCKEMGFNGMRKHMMVADPRYLYWCDKL
GVLVWGEMAAAYVYNEQYAYRMIEEWKEVLERDYNHPSIVVWVPLNESWGVADIYYNEKQ
QHHAQSLYHITKSVDSTRLVISNDGWEHCKSDLCTVHDYCRDPEILERRYSSIDHILTDM
PGLEGRKFIYNPGFSYQGEPVLCTEFGGVNYQVEPSSPVVEPRAENEEQFIAQLVDVIHP
YVSSQLVQGYCYTQLTDTETEICGLLTWNREPKVPLEVIRKMNEGYREF

Enzyme Prediction     

No EC number prediction in MGYG000001535_00970.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	14	574	1.6e-110	0.601063829787234

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	2.17e-48	16	440	10	429	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	4.59e-42	19	407	13	417	beta-D-glucuronidase; Provisional
PRK09525	lacZ	1.06e-26	19	440	53	485	beta-galactosidase.
PRK10340	ebgA	3.48e-26	16	440	39	472	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02837	Glyco_hydro_2_N	1.95e-19	19	176	2	165	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AYQ74694.1	5.96e-299	5	585	4	583
QMV41052.1	1.37e-245	5	585	6	582
ADI26383.1	5.53e-245	1	585	1	579
AYQ71521.1	8.98e-245	5	585	7	584
AZN38609.1	9.31e-245	5	585	8	585

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SF2_A	7.50e-82	7	571	28	566	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_E Chain E, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_F Chain F, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
4JKM_A	1.59e-35	21	589	18	598	CrystalStructure of Clostridium perfringens beta-glucuronidase [Clostridium perfringens str. 13],4JKM_B Crystal Structure of Clostridium perfringens beta-glucuronidase [Clostridium perfringens str. 13],6CXS_A Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine [Clostridium perfringens str. 13],6CXS_B Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine [Clostridium perfringens str. 13]
6LEM_B	6.88e-32	19	407	11	411	ChainB, Beta-D-glucuronidase [Escherichia coli]
6LEJ_B	6.98e-32	19	407	13	413	ChainB, Beta-D-glucuronidase [Escherichia coli]
6LEG_A	7.02e-32	19	407	14	414	ChainA, Beta-D-glucuronidase [Escherichia coli],6LEG_B Chain B, Beta-D-glucuronidase [Escherichia coli],6LEG_C Chain C, Beta-D-glucuronidase [Escherichia coli],6LEG_D Chain D, Beta-D-glucuronidase [Escherichia coli],6LEL_A Chain A, Beta-D-glucuronidase [Escherichia coli],6LEL_B Chain B, Beta-D-glucuronidase [Escherichia coli]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P05804	3.82e-31	19	407	13	413	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
P77989	2.25e-30	21	415	19	396	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KPJ7	2.63e-28	6	413	41	436	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P26257	2.83e-26	51	529	40	478	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1
O97524	9.99e-26	8	447	29	487	Beta-glucuronidase OS=Felis catus OX=9685 GN=GUSB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000041	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001535_00970.