CAZyme Information: MGYG000001535_00990

Basic Information

• Species: Paenibacillus_A phocaensis
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus_A; Paenibacillus_A phocaensis
• CAZyme ID: MGYG000001535_00990
• CAZy Family: PL8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2140	MGYG000001535_9|CGC7	233823.48	4.6557

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001535	5515101	Isolate	not provided	not provided

• Gene Location: Start: 389674; End: 396096 Strand: +

Full Sequence      

MSKLLNSLMAFILLVGTWGSMSMNVVHASEPSTVNMDQTVVERGQPLTIHYTGAQGPQDW
IGLYRKDETPKKGTNAWKWAYVSDLPDGTTTLTDNSEGRKEYPSLKDLAPGEYYLAYLLD
DGYTENADRFYFQIVDKEVTQLKATPESVTLKLNPQEDSAQLQVEATYRTNETEDVTAAN
DIQYVSSLPQVATVTSTGLIKAVSAGTAVIDITFQGKSTTAEVTVDSTATGPDPKPEIEL
TVMSLNLWAGDAIKKNGKDRVVQFLENLIRTADADVIGIQESNDEVVTQAAASLGYHVSM
KTSPNVSLMSKYPILDVDKEKDYYLIEVDPGRAVAISNVHLSSSPYGPYSIADGKTVESV
LADENLKHMTEMRNPFSKLPALAGQGMPVFLTGDFNVPSHLDWTEATKDRYFGKVVEWPV
SKKLQELGMVDSYRSLHPDPVATPGITWAVEGTEWRTPEVYDRIDYVYAGGPATTLESKL
IGEKAVKGGPISYADIQLDPYLSDHRAVVSKFKVQPRSYADLNLPSTPKPDYSSTLSMDQ
TVIERGQPLTIHYTGAQGQKDWIGIYLKNETPKKGTNAWRWSYVNDLPDGTITLTDKSEG
RKEYPSLKDFPPGEYYLAFLLNDGYTEKANRFYFQMVDKASTPQEIADMKIMKQRMVDYY
ISRDIINDGTNGRVEWTFKSEAGTYLSSQQPDGSWGDVDYKSTTSAANGRGWSPYLALDR
MQSMAQAFADPSDPYYHNPELLKGIQKALDYWFTVKPTSTNWWETGIGKQLRLEKIALLC
EGYLTATQVSNIIDTLDSSPHTVDGANSSWYNQNYMYRGLLLEDARIVRNAVDAFNILSN
VTTTVTGIQSDMSFFMHGKTNYTTGYGRSFARDMSFWAYITSGTVFSYSEAAIDSLSSYL
LDGTRYLVKGDVADLGMGMNGPDWPDYASAALTFYEDPMQWMQTANPKRAAEFARFLENI
REIGTNTSNGLDANNMTQWQTLVASHMRTGYGITVKMASSTVKGGEWRTIHPSGYNLLYW
TPQGATAIQRTGDEYRPVYPLMDWAHVPGTTAPYTLTKDGNFNNPRTFVGGVTNERYGAT
AFDFNKLSTSGKKGYFFFDDEMVALGAGIASTNAAPIHTTLNQSQAVGEVLVDGHPIADG
TTQTTGRWAYNDNIGYVFPNPTEFQVQRETKTGAWSDVISGSSTTPMTKPIFSIWLDHGV
KPTNASYQYIVLPDKNVDEVSNYASDNPVSILSNTSSVQAVRHQSLGIAELLFYQPGTVT
VRDGLTVTVDQPAMVILDESTAPTRISVANPETPGITVNVTLDRNGTTSTTSFRLGKDTF
TGRSLTLNEGESIDDSGFDLAYSKGAVASSSKDNHFASNATDLYRTSYWISNDSDHEWIY
VDLQNQYSIQQVRLNWENAYGKSYKIQVSNDAVDWTEVYSTSTGNGGIDDITFSPVTARY
VRMQGVQQGAGNGYSLKEFNVFEAVPPNLAEGKPVTASSSKAADVKAENAVDGSLNTRWG
SNYSDPQWIYVDLGASKSIQEVHLHWESAYGKEYQIQVSDDAKNWTTVYNTTTGDGGVDK
ISFAPVNARYVQMYGTKRGTTYGYSLWEFKVFPALATAPDAPADVQAVGYDGAAVVSFAP
PIHNGGSEITGYQVTAWANGVAAATTEGSASPITVTGLTNGIAYTFTVIARNGTWESASS
APSNEVIPSPNGTNVPVPPVNLTAAPGDQSVTLRWAPSAESVTYSVYQYEGVGAPANPDN
WQRVQASVTEATYTVTGLTNGRSYTFAVKAADARGESDFSHAVTATPKASRPETPNPPQP
ETPVSSSETGYSKDTSIIESTNGSILIPKGRAGVVSLDQELTLTINTGAAEQELSIKIEK
LRSTADLVFDKVTLVSNIFELTKNTTGNFKKPIVLSMLFNPSLVRDNQRVAIFYYDEAEK
VWIEVGGIVNGNQITATVEHFTKFAVMAVDEKEDNTGEPYEPVPSFTDITGHWAQNAILS
AAGMKLVSGYPDGTFKPNSAVTRAEFTVMLANALNLEGAGTAAAAFTDEVRIGGWAKQAV
ANAVEAGIVSGYADGSFRPNERITRAEMAAMSARALKLTAEANATTGFSDDKNIPIWAKG
AIEAIRKLGIMSGHGDNEFVPNGKATRAEAVTLLVRMMEQ

Enzyme Prediction     

No EC number prediction in MGYG000001535_00990.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL8	984	1216	1.5e-59	0.9536679536679536
CBM32	1476	1590	7.7e-30	0.9274193548387096
CBM32	1347	1460	1.8e-24	0.9274193548387096

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd01083	GAG_Lyase	2.12e-119	651	1291	4	693	Glycosaminoglycan (GAG) polysaccharide lyase family. This family consists of a group of secreted bacterial lyase enzymes capable of acting on glycosaminoglycans, such as hyaluronan and chondroitin, in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen. These are broad-specificity glycosaminoglycan lyases which recognize uronyl residues in polysaccharides and cleave their glycosidic bonds via a beta-elimination reaction to form a double bond between C-4 and C-5 of the non-reducing terminal uronyl residues of released products. Substrates include chondroitin, chondroitin 4-sulfate, chondroitin 6-sulfate, and hyaluronic acid. Family members include chondroitin AC lyase, chondroitin abc lyase, xanthan lyase, and hyalurate lyase.
pfam02278	Lyase_8	3.14e-70	986	1216	12	252	Polysaccharide lyase family 8, super-sandwich domain. This family consists of a group of secreted bacterial lyase enzymes EC:4.2.2.1 capable of acting on hyaluronan and chondroitin in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen.
pfam00754	F5_F8_type_C	2.11e-23	1474	1588	1	125	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam00754	F5_F8_type_C	9.76e-20	1346	1458	2	125	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
NF033190	inl_like_NEAT_1	8.88e-19	1953	2140	569	754	NEAT domain-containing leucine-rich repeat protein. Members of this family have an N-terminal NEAT (near transporter) domain often associated with iron transport, followed by a leucine-rich repeat region with significant sequence similarity to the internalins of Listeria monocytogenes. However, since Bacillus cereus (from which this protein was described, in PMID:16978259) is not considered an intracellular pathogen, and the function may be iron transport rather than internalization, applying the name "internalin" to this family probably would be misleading.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QMV41393.1	0.0	1	2139	1	2235
CQR51520.1	0.0	640	2139	39	1610
QQZ62770.1	0.0	649	2139	1	1563
QUL55219.1	0.0	637	2139	12	1488
AZM50462.1	3.55e-256	640	1592	34	972

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1CB8_A	5.70e-67	686	1291	32	639	CHONDROITINASEAC LYASE FROM FLAVOBACTERIUM HEPARINUM [Pedobacter heparinus]
1HM2_A	8.74e-67	686	1291	54	661	ACTIVESITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HM3_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-Oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus],1HMU_A ACTIVE SITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HMW_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus]
6F2P_A	6.64e-47	695	1307	58	713	Structureof Paenibacillus xanthan lyase to 2.6 A resolution [Paenibacillus]
2E24_A	1.57e-46	689	1260	37	668	ChainA, Xanthan lyase [Bacillus sp. GL1]
2E22_A	2.80e-46	689	1260	37	668	Crystalstructure of xanthan lyase in complex with mannose [Bacillus sp. GL1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q59288	4.78e-66	686	1291	54	661	Chondroitinase-AC OS=Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3) OX=485917 GN=cslA PE=1 SV=1
Q9AQS0	6.21e-45	689	1260	62	693	Xanthan lyase OS=Bacillus sp. (strain GL1) OX=84635 GN=xly PE=1 SV=1
P38536	1.29e-33	1812	2140	1544	1857	Amylopullulanase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=amyB PE=3 SV=2
Q9GUC9	9.93e-26	240	472	27	274	Uncharacterized protein F14F9.5 OS=Caenorhabditis elegans OX=6239 GN=F14F9.5 PE=1 SV=2
P38535	4.36e-23	1965	2140	907	1083	Exoglucanase XynX OS=Acetivibrio thermocellus OX=1515 GN=xynX PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000317	0.998973	0.000211	0.000181	0.000165	0.000152

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001535_00990.