CAZyme Information: MGYG000001542_02787

Basic Information

• Species: Paenibacillus_A sp900069005
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus_A; Paenibacillus_A sp900069005
• CAZyme ID: MGYG000001542_02787
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase BoGH2A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
814		91746.35	7.1381

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001542	5846629	Isolate	not provided	not provided

• Gene Location: Start: 158777; End: 161221 Strand: -

Full Sequence      

MARTSFNDHWQFWKEGQTEVKEVDLPHDAMIWEQRDPHCDNAHNSGFYPGSKYYYKKIWN
IPNQFAGKRLTLEFEGVYRNAVVLINGKQAAERPSGYTTFYVDCTEYVRYGEENEVLVIA
DNKDTPNGRWYTGSGIYRPVNLIVKDEHHIHINGVKVKTLSHQPPRIEVRIEHSGGGVVI
EVYYKGKLMTRANGDCAVMDIPDGKLWSDETPELYQCKVMLEHNGRIVDEEWVDVGIRTL
SWSTDGFLVNGKETKLRGACIHHDNGILGACAFDDAEERRVRLLKKAGFNAIRSSHNPCS
RALLQAADKVGMYVMDEFSDVWVVHKQKNDYASDFDKWYMEDLRAMVVRDYNHPSVVMYS
IGNEIGEVWEERGIQLTKEMTEVCHRLDDSRPVTCGTNLMLANMAMKGIGVFKVVDVDDK
KRPAKPDKKKFKLVASEFYNILINIMGGMMDRQSKKKNCDKATAEHFSHLDICGYNYGTI
RYANEPRLHPERIIVGSETRPPNIMENWRATMNNKYIIGDFMWVGWDYLGEAGIGAIAYS
SRSPSGWLLKKYPYLLADAGVIDICGWFRPEVWLNRVAWGTIGNNPYIGVEPVTHAKEKV
IRSMWRKSDAVHSWSWHGYEGSPAKIVVYSRAKTVELLLNGKSLGKKPIKEGAALFKTKY
LPGELVAVNYDANGKELGKNHLRTGGKDIKLTLDPEKTVLRANGQDLCYVNIYLTDGHGE
ILPATDRSVHVQVSGAGQLLAIGSANPYTEEGFVSGNHQTYYGRALAVIRVGRNAGLIQV
TISADGVEDKTIAIDVFDAVEVQYSGIPGDGTLC

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	551	1.4e-100	0.5797872340425532

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	9.18e-66	54	402	85	438	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	3.86e-39	54	403	68	454	beta-D-glucuronidase; Provisional
PRK10340	ebgA	3.16e-31	55	393	113	472	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam18565	Glyco_hydro2_C5	1.09e-30	691	792	1	102	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK09525	lacZ	1.24e-25	103	366	173	464	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEE97750.1	9.88e-261	1	796	1	784
CBK92219.1	7.82e-243	1	796	1	791
AFN84582.1	2.98e-231	1	796	1	769
QRT50826.1	5.66e-227	1	796	1	794
QRT30385.1	1.47e-221	1	803	1	803

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6B6L_A	9.36e-143	1	794	5	775	Thecrystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838],6B6L_B The crystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838]
7RSK_A	3.98e-142	1	794	5	775	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7RSK_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
5T98_A	1.41e-105	5	796	29	820	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
4YPJ_A	1.76e-99	3	794	9	805	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
7CWD_A	1.99e-98	1	794	1	799	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	1.65e-98	3	794	44	846	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	6.08e-68	4	747	49	770	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
P77989	7.43e-40	5	751	7	702	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KPJ7	4.35e-35	2	687	52	705	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P26257	2.41e-30	7	477	8	494	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000056	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001542_02787.