CAZyme Information: MGYG000001543_02349

Basic Information

• Species: Lachnoclostridium sp900078195
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnoclostridium; Lachnoclostridium sp900078195
• CAZyme ID: MGYG000001543_02349
• CAZy Family: GH3
• CAZyme Description: Beta-glucosidase BoGH3B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
734	MGYG000001543_7|CGC9	81744.53	4.8756

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001543	4599950	Isolate	not provided	not provided

• Gene Location: Start: 780000; End: 782204 Strand: +

Full Sequence      

MDQQKLKELVSDMSLTEKINQLLQVTSGFFVNEAELTGPIRENGITEESISEAGSIIGLL
GAKKYKEIQDAYIEKHPHKIPLLIMLDVINGFRTIFPIPLAQGATFEPELSKKCAKIAAK
ESAVSGVHVTFAPMVDLVRDARWGRVMESTGEDTKLNSDFAAAMVEGFQGNHVGEEYKIA
ACVKHFAAYGAPTAGREYNTVELSENTLRGYYLPAYEAAIKAGVEMVMTSFNTLNGVPAT
GNQWLMRDVLRDEMKFDGVLISDWAAIEELIYHGYAKDRKAAAGLAMNAGVDIDMMTGIY
SQNLVSLIEDGTVKEEILDEAVLRILQLKNKLGLFENPYKDADEEKEKEVILCEEHRQVS
LEAAEKSFVLLKNEGILPLNKKEKVALIGPFVHEKEIYGTWSMLGRPEDTVSIANAAEKY
RVDYELTFAKGCDILGEEDIDLIPEVHGKAMKLENQETLLKEAVEAAKNADKVILALGEH
RRMSGEAASRAELTLPKVQERLLHAIYEVNPNIGVVLFTGRPLDLRLVSEKSKAILNVWM
PGTEGGNAILNVLTGKVSPSGKLPMSFPYCVGQVPVHYNEYFTGRPYDPKSDGKEYRSNY
RDIPNSPLYPFGYGLSYAKFQYSDLKLSKDTMQEGETLEACIKIKNEGEYSGTETVQLYI
RDCVGSVVRPRKELKAYQKVDLKPGEVKCVTFKITEEMLQFCKADLTFGSEKGEFHVFIG
TDSNTQEYVSFELI

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	75	296	9.7e-67	0.9814814814814815

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	0.0	8	733	40	764	beta-glucosidase BglX.
COG1472	BglX	4.86e-104	13	402	1	372	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam00933	Glyco_hydro_3	8.35e-78	14	328	1	316	Glycosyl hydrolase family 3 N terminal domain.
PLN03080	PLN03080	5.34e-74	94	721	112	763	Probable beta-xylosidase; Provisional
pfam01915	Glyco_hydro_3_C	1.07e-57	369	617	2	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ABX41086.1	0.0	1	733	10	742
ASU29936.1	0.0	1	734	1	734
QQQ94715.1	0.0	1	734	1	734
ANU77139.1	0.0	1	734	1	734
QJU17237.1	0.0	1	734	1	734

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4ZOA_A	3.69e-210	1	733	1	719	CrystalStructure of beta-glucosidase from Listeria innocua in complex with isofagomine [Listeria innocua Clip11262],4ZOA_B Crystal Structure of beta-glucosidase from Listeria innocua in complex with isofagomine [Listeria innocua Clip11262],4ZOB_A Crystal Structure of beta-glucosidase from Listeria innocua in complex with gluconolactone [Listeria innocua Clip11262],4ZOB_B Crystal Structure of beta-glucosidase from Listeria innocua in complex with gluconolactone [Listeria innocua Clip11262],4ZOD_A Crystal Structure of beta-glucosidase from Listeria innocua in complex with glucose [Listeria innocua Clip11262],4ZOD_B Crystal Structure of beta-glucosidase from Listeria innocua in complex with glucose [Listeria innocua Clip11262],4ZOE_A Crystal Structure of beta-glucosidase from Listeria innocua [Listeria innocua Clip11262],4ZOE_B Crystal Structure of beta-glucosidase from Listeria innocua [Listeria innocua Clip11262]
4ZO6_A	5.93e-209	1	733	1	719	CrystalStructure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with cellobiose [Listeria innocua Clip11262],4ZO6_B Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with cellobiose [Listeria innocua Clip11262],4ZO7_A Crystal structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with gentiobiose [Listeria innocua Clip11262],4ZO7_B Crystal structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with gentiobiose [Listeria innocua Clip11262],4ZO8_A Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with sophorose [Listeria innocua Clip11262],4ZO8_B Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with sophorose [Listeria innocua Clip11262],4ZO9_A Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with laminaribiose [Listeria innocua Clip11262],4ZO9_B Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with laminaribiose [Listeria innocua Clip11262],4ZOC_A Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with sophorotriose [Listeria innocua Clip11262],4ZOC_B Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with sophorotriose [Listeria innocua Clip11262]
5XXL_A	4.42e-185	9	733	17	751	Crystalstructure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXL_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXM_A Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482],5XXM_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482]
5TF0_A	1.88e-184	9	725	16	742	CrystalStructure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393],5TF0_B Crystal Structure of Glycosil Hydrolase Family 3 N-Terminal Domain Protein from Bacteroides intestinalis [Bacteroides intestinalis DSM 17393]
5XXN_A	2.49e-184	9	733	17	751	CrystalStructure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXN_B Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXO_A Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482],5XXO_B Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q56078	5.64e-175	8	734	40	765	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
P33363	1.12e-171	8	734	40	765	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
T2KMH9	2.73e-119	3	733	37	754	Putative beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22230 PE=2 SV=1
T2KMH0	8.83e-119	82	733	77	720	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
A7LXU3	1.51e-108	6	720	39	762	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000052	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001543_02349.