CAZyme Information: MGYG000001543_02614

Basic Information

• Species: Lachnoclostridium sp900078195
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnoclostridium; Lachnoclostridium sp900078195
• CAZyme ID: MGYG000001543_02614
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1192	MGYG000001543_7|CGC16	135772.83	4.7129

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001543	4599950	Isolate	not provided	not provided

• Gene Location: Start: 1123922; End: 1127500 Strand: +

Full Sequence      

MGSIRLFNDEWTFLKTKLDVTKEEALANLEQFRQVDIPHDWLIYNVHNLYEDSCGWYYKK
FNLSSTLESLGSVFLRFDGVYMDSTVYVNNQKVGDWKYGYSAITHDITKALRNGENEVLV
QVRFQSPNSRWYSGAGIFRNVYIKECSRAFLPLDGTYISINEQGEDYLLEVETEVEGQIT
EDIMCHYSLWFENEMIQDFGMQKVSMLKESSNVNDSNSAVLKESSEVNGSESASLKDSSN
INGSKFASLKEIIKNPRIWDVDSPQCYELQVELYDTSCNECLDSERITIGFRSMKFNPEL
GFILNGRQLKVHGVCEHHDFGCLGAAFYKEAMRRKFKILREMGVNALRTSHNMPAKEVME
LADEMGFLIVNEAFDMWERNKTEYDYARFFIEWAKEDVKSWIRRDRNHPSLMLWSIGNEI
YDTHVDEHGQEITERLVWLVREHDPKENALITIGSNYMPWENARKCADIVKIAGYNYAEK
YYEAHHKEYKDWVIYGSETASLVQSRGVYKFPLSKPILSDEDEQCSALGNSTTSWGTKNA
EQCIIDDRDAKFCFGQFIWTGFDYIGEPTPYHTKNSYFGQLDTAGFPKDAYYIFKAEWTD
AKKNPMVHVFPYWDFNEGQLIDVRACTNGAAVELFVNDISYGCQAINHETGKKLLGEWKV
PYSPGTIKAIAYDIDGEPIAEEVRNSFLDAKQITLSADKTKLLANGEDLCFLTIAVEDEF
MNPVENAMNYVTVTLDGPGRLLGLDNGDSTDYDEYKSNVRKLFNGKLLAVIGTTCKAGEI
KVTLSGEGLKSSTITLTLVESKIREEICAREDFRSYDRSEKGHPIIPVRKIELSLKEGNE
LNENVKELMCKAYIYPEDASDTNLIWKVVNDSGIEISYAQVEEIKNEGNVHIAKVKAIGD
GDFYVRCMSKSGTDKIRIISSMNFHAKGIGRAFLNPYEFIYAGLCTDTIGDIGNGNEKGI
STSRDGISGVIYKNLDFGDYGSDEITIPIFALTSDEYEIEIWQGSPNELGSEKLTTVTYQ
KESIWNTYQEETYLLPRRIKGIATIAFMLKEKVHIKGFKFRKLNKAISKVNAAECNRVYG
DDFIIESEAVTGIGNNVTLEYENMDFGSEGVSKIEITGRTPLSVNTIHIHFTKEDGTKQN
CIVEFDKRNENSTNLDYVSQLFEIDKLEGKGTVAFVFLPGSNFDFNSFQFIR

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	4	612	7.8e-100	0.6156914893617021

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	8.17e-59	31	614	61	614	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam18565	Glyco_hydro2_C5	1.28e-38	693	795	1	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10150	PRK10150	2.97e-29	56	445	68	441	beta-D-glucuronidase; Provisional
PRK10340	ebgA	3.52e-24	51	419	107	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	6.08e-22	3	419	51	462	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QZN78011.1	0.0	1	1190	1	1160
APO44079.1	0.0	1	1190	1	1155
SQI53931.1	0.0	6	1190	6	1140
QLG36749.1	0.0	1	1190	1	1157
QOS77051.1	0.0	1	1190	1	1167

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	4.11e-164	7	796	6	799	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	4.96e-153	7	799	12	808	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
4CU6_A	2.79e-146	7	796	18	842	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	7.63e-146	7	796	18	842	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]
5T98_A	1.58e-132	7	798	30	820	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	3.02e-146	7	800	47	850	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	4.04e-80	7	766	51	788	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	1.24e-40	8	709	57	744	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	3.13e-33	7	780	8	719	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KN75	2.81e-30	7	796	31	793	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000073	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001543_02614.