CAZyme Information: MGYG000001548_01491

Basic Information

• Species: Paenibacillus_A tuaregi
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus_A; Paenibacillus_A tuaregi
• CAZyme ID: MGYG000001548_01491
• CAZy Family: GH3
• CAZyme Description: Beta-hexosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2394	MGYG000001548_4|CGC25	254988.22	4.9491

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001548	5663842	Isolate	not provided	not provided

• Gene Location: Start: 1588789; End: 1595973 Strand: +

Full Sequence      

MKKRKLSIWLSGLTAFSLILGLLPLPAVLGAPTAAANRLPAYLNPGESIPERVADLLNRM
SLDEKVGQMVQAERASVTPEDVKDYYLGSVLSGGGSFPEGKQTSSTQEKWTALYNSYQDG
ALSTPLGIPLLYGVDGLHGHNNVIGATLFPHNIGLGATRDPGLVKKIGAASAAEMQATGV
NWTFAPTLAVVKNPRWGRTYEGFGDSPALSTELGAAYIQGLQGEKPGGLKDPGKVVATAK
HFAGEGYTDNGTNQGNITAYTEEQIWNLEKEVYEAAVKAGVKTVMASYHSIQGIKMHANQ
RLLTDKLKGELGFKGFVISDYNAVDQITRDWDGKAVSGLKNQVRVAVNAGVDMIMEPDKW
KEVIVDLKELVQEGAISEKRLDDAVSRILQVKFESGVFEHPKADPDLAGAFGSSSSRSVA
REAVQKSLVLLKNDKVANGGGGQSPILSQLPSMKKILVAGKSADDIGLQSGGWSITWQGS
AGDITPGTTILKGIQDAAGSSRTVIYNKHGRGAAGQDVAIAVIGEKPYAETNGDAASLNL
DAEDLNTLANIRSGNPDIPIVVVLVSGRPMTITNQMGDWAGLIAAWLPGTEGQGVADVLF
GSKDFTGKLPMRWPFSLNAYPGGDASQFLFQAGYGLTRNQPTPTLPSAPEEPDHSQKIPG
KIEAENFTAQSGIQTEDSQDTGGTRNISYIDAGDWLEYDVDAQSGTYKVDFRYSSWTDST
GLKIIDGTGKTRGTLSMSKTGSYQDWQTASIENVSLPKDGKQKLKLQFTGGSMNLNWFSF
TWTGEAAPDNGGDPGGGGTDGVTPVVVEAGAVESWITNERNPGDNKWYYDSLYKDGDKKL
EQQPNADIVQPNQAKGTEITIDPDQTYQSVVGIGSSMEESTIGNLWLMSEAKRKEVLMKL
FSKTEGAGMSLVRLTIGTADFTGKPFYSYDDLPAGQTDPGLTHFSIQKDIDYHIIDTIKE
IQKINPDVKFFASSWSPPGWMKTTDSMIKGQVKEEYLPVLADYFVKYVQAYKALGINIDA
LTMQNEPLLEIEYPSTHMPWDQQGRLAVLLRSKLDAAGFEDTKLWIFDHNPSDTSAYSVH
ILADEQMAGAVDGTAFHDYGGDLSEMSRLHDLYPDKNVYLTERAVWGTQGADRIAQYFRN
WARSYNSWVVMLDSDIKSHQWVGTPDPTPIIQDSADRNNYWLAPEYYLMGQFMKFVEPGY
VRIDSNYGSSSTVTNVAFKSPDGKTIVTVVINQTADDQPVKLLSDGKQISAVVPAKSVAT
YRWHRSEQGKTAPGSYPAADFDEATGQFKADKNTGIVDHLNVDGDGNSTAGSAEFKYNLH
VKQAGSYDLDLGYAGSLEDASLSAKVGDGAPVIIRENSKASEQPAGDAAGAPADGSAPAG
DAAGAPAGGSADESAPAGETAGAPAGRSAPTGTAPAKASVTEAVYGPGDFRYLRVRLDLP
AGEQKLILSAGGHNYALSDLRLTKADLDNTLPKKIQAENADQSSGILIQHQDANQLTSVT
KSAPEAWLEYKVKVPADGQYQLTYRYASEGKSPTAVWTADGTNEPVLTTVLTGSEGPNDW
KTVTDLIKLQEGSHTLRLSLKEAYIHLDWFQIGPLVLTNPGAQAISLPGTAQAGDYISAT
GISAVEGPSGSKKLTSFDAGDWVDYPITVQKGGDYTVTVELATKNGGMNAFNLKAEDGTV
LAKFDIPSTGDLNTWIRIRKTVALKSGEQKLRLSANTAGFEITKLAFEETTAHTPGQDGM
VKVEAESYFDADRNAIQTNSEYTNVGYLNRGSKLDYLVNIPASATGNYKVTYRYATPQSG
VSVNLSVDGGSAGTLQLGSTGGWGNYTEASHLVTMKEGTKTVRLLDQGDGFNLDWFTIVP
TDEVVKPEQVRTKIPAASLKAGSYDKPQTVSLMTGVEGARIYYTTDGTLPSAKNGTLYEG
PFQVSGIVVIRAVAVKDGLADSFVAPYTYVTKAGGGTDGNNGGNNGGNPGGNPGGNTGGS
NGGNQGGNKGGSTGNTGGSAPGTTDGKGILKLSAPTLDPVTGIAVAVVTTEGLNQAIKDA
GSSTSSKVTIIAEVPSVHGQKGYGVQLPASYFTGKKMVKLELVTEYGSILIQDNMIRPAE
VSANSQIELRIMPADKTKLAASAVKAIGEHPALELALLIGGQPVAWNNPDAAVRVRFPYQ
PSAAEAASDEHITVLYLDPQGRLNPVPNARYDAASGKVIFTTTQFGQYAVAYVTKTFGDI
AKFSWAKHDIEVLASKGIVKGTSDTTFNPGDQVRRADFLVMLVNALGLSAKTEGNFADVN
SSAYYYEAVGIAKQLGIAKGKENNQFDPLSPITRQEMMTLAARAMVLAGTTLPASSAAEL
NSFQDAEQVSGFAADSVAALVKSGIVNGDGHHLNPLSQTTRAETAVIIYRIYNK

Enzyme Prediction     

No EC number prediction in MGYG000001548_01491.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH30	855	1263	3e-139	0.9928057553956835
GH3	123	355	1.5e-73	0.9768518518518519
CBM6	663	781	1.5e-26	0.8623188405797102
CBM6	1744	1858	3.3e-19	0.855072463768116
CBM6	1613	1728	3.9e-18	0.855072463768116

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1472	BglX	9.18e-92	60	497	1	394	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
PRK15098	PRK15098	5.93e-85	13	621	4	607	beta-glucosidase BglX.
pfam00933	Glyco_hydro_3	4.04e-74	61	391	1	316	Glycosyl hydrolase family 3 N terminal domain.
COG5520	XynC	9.50e-46	859	1265	36	430	O-Glycosyl hydrolase [Cell wall/membrane/envelope biogenesis].
cd04080	CBM6_cellulase-like	4.75e-37	658	781	1	144	Carbohydrate Binding Module 6 (CBM6); appended to glycoside hydrolase (GH) domains, including GH5 (cellulase). This family includes carbohydrate binding module 6 (CBM6) domains that are appended to several glycoside hydrolase (GH) domains, including GH5 (cellulase) and GH16, as well as to coagulation factor 5/8 carbohydrate-binding domains. CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. The CBM6s are appended to GHs that display a diversity of substrate specificities. For some members of this family information is available about the specific substrates of the appended GH domains. It includes the CBM domains of various enzymes involved in cell wall degradation including, an extracellular beta-1,3-glucanase from Lysobacter enzymogenes encoded by the gluC gene (its catalytic domain belongs to the GH16 family), the tandem CBM domains of Pseudomonas sp. PE2 beta-1,3(4)-glucanase A (its catalytic domain also belongs to GH16), and a family 6 CBM from Cellvibrio mixtus Endoglucanase 5A (CmCBM6) which binds to the beta1,4-beta1,3-mixed linked glucans lichenan, and barley beta-glucan, cello-oligosaccharides, insoluble forms of cellulose, the beta1,3-glucan laminarin, and xylooligosaccharides, and the CBM6 of Fibrobacter succinogenes S85 XynD xylanase, appended to a GH10 domain, and Cellvibrio japonicas Cel5G appended to a GH5 (cellulase) domain. GH5 (cellulase) family includes enzymes with several known activities such as endoglucanase, beta-mannanase, and xylanase, which are involved in the degradation of cellulose and xylans. GH16 family includes enzymes with lichenase, xyloglucan endotransglycosylase (XET), and beta-agarase activities. CBM6 is an unusual CBM as it represents a chimera of two distinct binding sites with different modes of binding: binding site I within the loop regions and binding site II on the concave face of the beta-sandwich fold. For CmCBM6 it has been shown that these two binding sites have different ligand specificities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASS69272.1	0.0	9	2392	28	2333
QJC54477.1	0.0	25	2394	2	2300
QGG57758.1	0.0	2	2394	24	2344
AEI42025.1	0.0	5	2394	19	2637
AFH60457.2	0.0	24	2394	14	2600

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5M6G_A	8.23e-175	32	637	31	613	Crystalstructure Glucan 1,4-beta-glucosidase from Saccharopolyspora erythraea [Saccharopolyspora erythraea D]
6JGC_A	3.74e-154	42	639	9	604	Crystalstructure of barley exohydrolaseI W286Y mutant in complex with glucose. [Hordeum vulgare subsp. vulgare],6JGD_A Crystal structure of barley exohydrolaseI W286Y mutant in complex with methyl 6-thio-beta-gentiobioside [Hordeum vulgare subsp. vulgare]
6JG7_A	1.31e-153	42	639	9	604	Crystalstructure of barley exohydrolaseI W286F in complex with methyl 2-thio-beta-sophoroside [Hordeum vulgare subsp. vulgare],6JGA_A Crystal structure of barley exohydrolaseI W286F in complex with 4'-nitrophenyl thiolaminaribioside [Hordeum vulgare subsp. vulgare],6JGB_A Crystal structure of barley exohydrolaseI W286F mutant in complex with methyl 6-thio-beta-gentiobioside [Hordeum vulgare subsp. vulgare]
1LQ2_A	1.43e-153	42	639	5	600	Crystalstructure of barley beta-D-glucan glucohydrolase isoenzyme Exo1 in complex with gluco-phenylimidazole [Hordeum vulgare],1X38_A crystal structure of barley beta-D-glucan glucohydrolase isoenzyme exo1 in complex with gluco-phenylimidazole [Hordeum vulgare],1X39_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme exo1 in complex with gluco-phenylimidazole [Hordeum vulgare]
1EX1_A	1.57e-153	42	639	5	600	Beta-d-glucanExohydrolase From Barley [Hordeum vulgare],1IEQ_A Crystal Structure Of Barley Beta-D-Glucan Glucohydrolase Isoenzyme Exo1 [Hordeum vulgare],1IEV_A Crystal Structure Of Barley Beta-d-glucan Glucohydrolase Isoenzyme Exo1 In Complex With Cyclohexitol [Hordeum vulgare],1IEW_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme Exo1 in complex with 2-deoxy-2-fluoro-alpha-D-glucoside [Hordeum vulgare],1IEX_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme Exo1 in complex with 4I,4III,4V-S-trithiocellohexaose [Hordeum vulgare],1J8V_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme Exo1 in complex with 4'-nitrophenyl 3I-thiolaminaritrioside [Hordeum vulgare],3WLH_A Crystal Structure Analysis of Plant Exohydrolase [Hordeum vulgare subsp. vulgare],3WLJ_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with 3-deoxy-glucose [Hordeum vulgare subsp. vulgare],3WLK_X Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with 4-deoxy-glucose [Hordeum vulgare subsp. vulgare],3WLL_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with PEG400 [Hordeum vulgare subsp. vulgare],3WLM_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme exo1 in complex with octyl-O-glucoside [Hordeum vulgare subsp. vulgare],3WLN_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with octyl-S-glucoside [Hordeum vulgare subsp. vulgare],3WLP_A Crystal Structure Analysis of Plant Exohydrolase [Hordeum vulgare subsp. vulgare]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
C6CRV0	3.15e-82	2005	2392	1075	1459	Endo-1,4-beta-xylanase A OS=Paenibacillus sp. (strain JDR-2) OX=324057 GN=xynA1 PE=1 SV=1
A7LXU3	6.53e-73	48	619	34	622	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1
Q23892	6.77e-73	53	637	80	698	Lysosomal beta glucosidase OS=Dictyostelium discoideum OX=44689 GN=gluA PE=1 SV=2
Q56078	2.31e-61	53	621	38	607	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
P33363	4.16e-61	53	621	38	607	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001288	0.997834	0.000246	0.000244	0.000180	0.000168

TMHMM  Annotations     

start	end
7	29