CAZyme Information: MGYG000001549_03942

Basic Information

• Species: Gracilibacillus phocaeensis
• Lineage: Bacteria; Firmicutes; Bacilli; Bacillales_D; Amphibacillaceae; Gracilibacillus; Gracilibacillus phocaeensis
• CAZyme ID: MGYG000001549_03942
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1014		113579.93	4.247

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001549	4547476	Isolate	not provided	not provided

• Gene Location: Start: 1691846; End: 1694890 Strand: +

Full Sequence      

MDIKEFIHKCWTSMKRIWSKQIIQRSSRISYHIIWNIFLFFIAVGVIGGFLATGIGAGYF
ASLVASTDIQSEDEMTQAVYNYEETSELYFSDDVFLSEVSADILRDEAELENVSEYVQQA
VIATEDEYFEKHNGIVPKAIMRAVFQEVTNASTKTGGSTLTQQIIKNQLLTNEVSFERKA
KEMLLAMRLEQFFEKDEILEAYLNIVSFGRNASGQNIAGIETAAEGIFGVNASELNLAQS
AFIAGLPQSPSAYTPFLNGGGVKDEEALQPGLGRMKSVLNRMKEEGYITEEEHQEALEYD
IVSDFKEPEESVIENYPYLMQELQDRAIDILVTVLAEEDGYTEEDLEGSSVLQEEYQIKA
SREIGSNGYKIHSTIDKEIYDVFQQVTKDYTNYGRDKLARNVNTNQPIMVEDPDTEEEVQ
LGQQPVQVGSVLMENNTGKILSFVGGRDFEISQRNHATNVSRQNGSTMKPLAAYGPGIEK
GAVQPGSVIADVKKTYGSGYSPGNYSSRYYGLTPVREALYRSHNATAVSVFDQIKGADIT
TDFLWKMGLTNFTEEQGGYNSLPLGSINVTIEQNTSAYSTFGNNGKHVEGYMIDKIETKD
GEVVYQHETEETEVFNPKTTYLMLDMMRDVLTQGTGTAARANLANKSVDWAGKTGTSNDY
RDAWFVASNPNVTLSSWMGYDYNQRLDDGYSNRNNTFWARLVNAATEVKPEIMAPSERFE
QPEGIVSRSYCTVSGLSPSDACSKLGLVQSDIYDADHVPSEKDYSLVNSSVSSIGGNSVL
AGDNTPSEFTDGDGVSFNPQWLKDMGYDKLEDIRQLTATKSGAWQKIQYPNMSSSDISDD
GEAPSAPSGLSEENQQLSWNKSGSSDVVGYRIYQTSNPDNDDYKQVGSTADTSYKVPDDD
AVYIVRAVDYFGKESEPSNRIVKGDFSSDSEEEEEKKEQEEQKKKEEEKKKQEAEKKKQE
EQKKQEEQKKQEEEKKKQAAEKKKQEEEQKKQEQEQQEQEQQEEAEDSGEDSEE

Enzyme Prediction     

No EC number prediction in MGYG000001549_03942.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	104	283	1.3e-55	0.9322033898305084

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	1.64e-159	28	683	10	574	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	2.94e-153	105	681	2	502	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	5.55e-131	34	745	8	753	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
COG4953	PbpC	8.06e-74	102	742	55	592	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].
pfam00912	Transgly	1.94e-67	105	283	13	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGH35346.1	0.0	1	931	1	932
QDP40889.1	0.0	4	927	3	921
BAM47039.1	0.0	20	929	20	936
APC47999.1	0.0	1	936	1	937
QTN00097.1	0.0	1	932	1	938

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2JE5_A	5.83e-128	53	738	2	665	StructuralAnd Mechanistic Basis Of Penicillin Binding Protein Inhibition By Lactivicins [Streptococcus pneumoniae R6],2JE5_B Structural And Mechanistic Basis Of Penicillin Binding Protein Inhibition By Lactivicins [Streptococcus pneumoniae R6]
4OON_A	3.93e-62	120	737	54	749	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
2FFF_B	1.42e-59	320	738	8	400	OpenForm of a Class A Transpeptidase Domain [Streptococcus pneumoniae]
2XD1_A	1.47e-59	312	738	37	439	ACTIVESITE RESTRUCTURING REGULATES LIGAND RECOGNITION IN CLASS A PENICILLIN-BINDING PROTEINS [Streptococcus pneumoniae R6],2XD1_B ACTIVE SITE RESTRUCTURING REGULATES LIGAND RECOGNITION IN CLASS A PENICILLIN-BINDING PROTEINS [Streptococcus pneumoniae R6]
2BG1_A	1.47e-59	312	738	37	439	Activesite restructuring regulates ligand recognition in classA Penicillin-binding proteins (PBPs) [Streptococcus pneumoniae R6],2XD5_A Structural insights into the catalytic mechanism and the role of Streptococcus pneumoniae PBP1b [Streptococcus pneumoniae R6],2XD5_B Structural insights into the catalytic mechanism and the role of Streptococcus pneumoniae PBP1b [Streptococcus pneumoniae R6]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7GHV1	5.53e-70	30	787	12	721	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) OX=441772 GN=pbpA PE=3 SV=1
A5I6G4	1.99e-69	30	787	12	721	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=pbpA PE=3 SV=1
A7FY32	1.99e-69	30	787	12	721	Penicillin-binding protein 1A OS=Clostridium botulinum (strain ATCC 19397 / Type A) OX=441770 GN=pbpA PE=3 SV=1
O66874	5.92e-69	110	726	68	684	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
Q0TNZ8	2.36e-68	49	744	34	699	Penicillin-binding protein 1A OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=pbpA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999974	0.000070	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
33	55