CAZyme Information: MGYG000001549_04319

Basic Information

• Species: Gracilibacillus phocaeensis
• Lineage: Bacteria; Firmicutes; Bacilli; Bacillales_D; Amphibacillaceae; Gracilibacillus; Gracilibacillus phocaeensis
• CAZyme ID: MGYG000001549_04319
• CAZy Family: GH136
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1908	MGYG000001549_12|CGC25	209042.31	3.7757

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001549	4547476	Isolate	not provided	not provided

• Gene Location: Start: 2143133; End: 2148859 Strand: +

Full Sequence      

MKRGKQLLVLSMICILFLFAIGYQEVAAAPADDTVIKAEALYGATVRGGSYADQSAETGS
NSIPREILEAKTAGNESYSRRSHLEFDIPSDTNWEETAAVMLSLYLHEHTGDGKEDTVQV
LLTDKADLSEENWTWNNTDDLLNNAEMIGEQTFTEEDADTWVEIDVTAIKEHLQANPDKD
FGIVLRVEEENDQGGIRFYSAYQDNGVYTPYLHIFQDEYQDTLPPRVQVTGVEDGATVTD
SEVDVNIQATDNHDEEPTIHLTVNGDKVEGAQQGVNVIQLKEGKNTIEVYAEDAAGNTSV
TQTVEVVWEAVTAFPVIADTYTDYRSPDQNHNGGNLDLKKAASDDSNQRETYLSFDLSDY
DAPYVKEATIQFYINELMGNDRTSELVSIYQVEGFEEENVTWNNAPERGQLVAEASYERT
ENNTYGELDISAFINEELQQTEGVGTLHLVLAIDNGHDQKGVFINSKEQDQDKAARIQLV
EGLPAPEVNIEGIEDGKTYTADTLTDVKIETTSVNESIDIQTEVLVNGEAVAEKADHLYD
LPLQLGENLIEITATDQEGNQTEKTLKVTRLEQGESTVYYIDSEAGDDANDGLTEDTPWQ
SLERLNHVQFQPGSSILFKRGGSWQGQFRPYGSGTADQPITIDAYGEGEAMPKIEGNGIS
NADTGSIYSEGAIHLYDLSHWRIQNIEVTNNGEEIAEAARAGIIVIAGGQGDVENVHIDN
VHVHDVNSGVDAEKLSGGIIFSSDTRDEQGNVTDIESSFHNISVENSHIHDVAIEGVRTK
TRRNGSDTGSIHSSDVVIRKNVIKDTFGDAIVMAEVGSGGLVEKNIVRRHSSNNNNRNYA
GVWLYQSHDAVIQYNEVTDGVYGYNDGEAFDFDIGTTDSVFQYNYTRNNRGGLLLTMSSA
GQNNVFRHNISYNDGQGTELFYVMNNRTQIYNNTIYVGEEVATEYVVKDNPIDYMFFKNN
ILFVEGEIEAYSQQPGSFEAPNMSHNLIYPAELLELNGSPNPYPGLIHEDPKFVSNGPDY
TEMDTWDEEIWNQNIAAFQLQDDSPAIGAGTAIDGMGSEDMLGNPIDSDNPTIGALQHTV
EEIELSEPEVTVDNEAWSNAESVGVSIEVTGDDVTAEYQLNGTEGEWLSYDGDLQITEEG
ETTVYARAVDASGNTSDVAEAVVKLDRTMPENPVVELSEDGWTSSDRVEVNIESADDPLS
GVAYTEYRMGKEGEWQPYEDAFAITTEGETVIEARTIDNAGNQSGVTEATAQIKRTAPSA
PMIEPETSDWSNAASVSVSITGESDKRVVEYQLSDTEGEWLSYAEPIAITEEGETTVYAR
AVDQAGNVSEVAEAVVKIDRTKPELSLHGDNPLYVLQGTDFEDPGYEATDNFTKEPEVTV
EGAIDTEEIDQQELVYTAKDTAGNQTSVTREVVVYEATQPSITLQGDNPLMIEAGTSYEE
PGATAEDEEDGDLTDQIEITGEVDESTPGSYALVYQVTNRAGLTTTVTRYVEVVDTQAPE
LTLDGEETVTVELGEEYQEAGATAVDAIEGDVSEAITISGAVDPMKAGIYQLNYTVQDSS
GNSTSVTRTVHVKDTTAPANVHLEVIDASEDAITLTFSAEDLAGIKQYILSRDGEEIAVV
DGEESTYIDTEVESGIAYSYQLVAVDPSENQASSEPVEVTTDTTERESPEVELDKEAEPV
VPGDMIAIKGTNTSIQLPQDLPEGTTLQVKKVKDLEMAGLELAGEVYDFIFTYPEGEEDY
QGNFILTMGVNDGVEQAAIYHFNEESNEWEKLGGERNGQQISQEVSHFSIYGVLAEVQEE
DSDEEETSNEEENQPPIDDEGGELPVDSDENSADQPKETNPEEISNTDNEKSGDVRESDQ
NETQTGASQEQLPNTATNMFNWLAAGGGLLLVSVVLLAMKRFRKRTEG

Enzyme Prediction     

No EC number prediction in MGYG000001549_04319.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH136	576	1076	5.3e-117	0.9938900203665988

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam16403	DUF5011	1.40e-23	1422	1493	1	71	Domain of unknown function (DUF5011). This small family of proteins is functionally uncharacterized. This family is found in Bacteroides, Prevotella, and Parabateroides. Proteins in this family are around 230 amino acids in length.
pfam16403	DUF5011	4.55e-23	1501	1572	1	71	Domain of unknown function (DUF5011). This small family of proteins is functionally uncharacterized. This family is found in Bacteroides, Prevotella, and Parabateroides. Proteins in this family are around 230 amino acids in length.
pfam16403	DUF5011	1.14e-13	1345	1414	1	71	Domain of unknown function (DUF5011). This small family of proteins is functionally uncharacterized. This family is found in Bacteroides, Prevotella, and Parabateroides. Proteins in this family are around 230 amino acids in length.
NF033679	DNRLRE_dom	1.02e-10	318	472	1	143	DNRLRE domain. The DNRLRE domain, with a length of about 160 amino acids, appears typically in large, repetitive surface proteins of bacteria and archaea, sometimes repeated several times. It occurs, notably, three times in the C-terminal region of the enzyme disaggregatase from the archaeal species Methanosarcina mazei, each time with the motif DNRLRE, for which the domain is named. Archaeal proteins within this family are described particularly well by the currently more narrowly defined Pfam model, PF06848. Note that the catalytic region of disaggregatase, in the N-terminal portion of the protein, is modeled by a different HMM, PF08480.
NF033679	DNRLRE_dom	2.43e-06	77	212	28	161	DNRLRE domain. The DNRLRE domain, with a length of about 160 amino acids, appears typically in large, repetitive surface proteins of bacteria and archaea, sometimes repeated several times. It occurs, notably, three times in the C-terminal region of the enzyme disaggregatase from the archaeal species Methanosarcina mazei, each time with the motif DNRLRE, for which the domain is named. Archaeal proteins within this family are described particularly well by the currently more narrowly defined Pfam model, PF06848. Note that the catalytic region of disaggregatase, in the N-terminal portion of the protein, is modeled by a different HMM, PF08480.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNK57279.1	0.0	5	1244	2	1261
AIC91360.1	3.16e-68	579	1083	87	683
AFU70744.1	8.19e-68	579	1147	87	750
QXN79520.1	4.74e-66	577	1117	52	693
QNR83147.1	4.79e-66	577	1077	48	517

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQC_A	6.76e-39	567	961	8	473	Crystalstructure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_C Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_D Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_E Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_F Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_G Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQC_H Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, ligand-free form [Bifidobacterium longum subsp. longum],5GQF_A Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, lacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQF_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, lacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQG_A Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, galacto-N-biose complex [Bifidobacterium longum subsp. longum],5GQG_B Crystal structure of lacto-N-biosidase LnbX from Bifidobacterium longum subsp. longum, galacto-N-biose complex [Bifidobacterium longum subsp. longum]
7V6M_A	1.76e-37	572	989	4	482	ChainA, Fibronectin type III domain-containing protein [Tyzzerella nexilis]
7V6I_A	2.67e-35	572	974	9	497	ChainA, Lacto-N-biosidase [Bifidobacterium saguini DSM 23967]
6KQT_A	2.27e-34	579	952	247	662	CrystalStructure of GH136 lacto-N-biosidase from Eubacterium ramulus - native protein [Eubacterium ramulus ATCC 29099]
6KQS_A	3.02e-34	579	952	247	662	CrystalStructure of GH136 lacto-N-biosidase from Eubacterium ramulus - selenomethionine derivative [Eubacterium ramulus ATCC 29099]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000312	0.998998	0.000215	0.000173	0.000151	0.000136

TMHMM  Annotations     

start	end
7	29
1879	1898