CAZyme Information: MGYG000001560_00025

Basic Information

• Species: Massilioclostridium coli
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Massilioclostridium; Massilioclostridium coli
• CAZyme ID: MGYG000001560_00025
• CAZy Family: CBM67
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1702	MGYG000001560_1|CGC1	187706.69	4.1457

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001560	2985330	Isolate	not provided	not provided

• Gene Location: Start: 29484; End: 34592 Strand: -

Full Sequence      

MKTKSKIRSILAAVLAVCLMLQMVVGLNITAFAADPALSVTNLEVESSVNPLGIDNTTPN
FSWKIDSSERGVTQKSYHIQVATSQENLKNGPYVWDSNVVESDQSVNIVYNGEALQPTTR
YFWQVTVTDNKGNTATSSEEAYWETGLMETNWSAKWIELDEGAQPSPANYSVEMDFRILK
DDIGIIFAAKDTSNFLMWQVNTFEKKFNGKTSFRPHQWVNNAASCIDEKSIDDVIPVEDQ
QKQHHIKIQVEGNQITTWIDDIQIDQRTSDLAAYGKIGFRQVTNAGDCDEHGSFDNIVVK
DTDTDDILFSENFDNGNRSFNFGTVNNGELEVYNTLGLQKDSMSSAPMYRKEFDVNKEIK
KATIHASALGIYELEVNGQKVSDDYFNPGWTNYELNQDDNNYVMYQTFDVTDLLQQGKNV
IGAMTGHGWYSGKLFVGGNNRYGTGSKLLCQVEVEYADGEKAIVAATDSSWKINGNGPII
EDDFQGGESYDANLEIPGWSTTAYTEDSSWYSAKESSYNGDIVAQMGPTVKAIQEFKPIE
VTNPSEGTYIFDLGQNIAGFARLHVKGEAGTTVKLRFGEMLNQDGTLYTDNLRSAKATDY
YTLKGDENGETYEPRFTFHGFRYIEVTGYPGVPTAEDITGIALSSLQETTGTFTTSNEWV
NQLQSNITWGQRDNFISVPTDCPQRDERLGYTGDGQVFVRTASMNMDVNQFFVKFMEDVI
SNQRADGAVADWTPNYVTPGDGMSGSFGNSGWGDAVVIIPWTMYTTYGNTDIIRQSYDGM
KKWVDRYNTMGGDSHILPSSCSAYGDWLSINADTPKEVIGTGYFAYCANLLSKMAEVIGE
TEDATYYHNLFLEVRDAFNKEFVDAEGHVKGQTQTSYLVALKFDLLPTEEARQKAADYLV
ADIKAKDWHLSTGFIGVAYLCPMLTEMGYSDVAYRLLLQDTYPSWLYSVKAGATTIWERW
NSYIAETGQFGDVGMNSFNHYSLGSIGEWFYNYVGGIMYDETNPGYKNVTINPYQGGDLE
FANTSYETPYGTIISNWKYVDNNTLQMDVQVPANSTATIYVPTEDGGIDNITENGAAIAE
SEGVEFVGMDDNKAMFKVGSGNYTIQTKVTREYLLNIADSNTAAASYVSINGGEMQKLPI
SEPVKAGDEITLKVTPVNDVDYQFSSISGAVESDKNEVTFTIEGTTNVTVTNSEIERVDL
ALNKTVNSSPSINNTDWNNKYLTDGNKISTTGSMGYTSTSTTTPDVNHWVEIDLGANTEF
NRIHIYPRTDVKTADGKTPSFPKDFTIQVKADGASEYTTIGTYNDYTAPEGKPAVLTFDE
LQNARYIRLNVTRVGDPPAGEPYYLQLAEMGIYNEVETPEPPEVDKTALETLYNQVKDTD
LNQYVDGEAKNTFVAELEKAKNLIANEEATQEQVNEAVTALQNAYDALEKKPVEPVETNK
DILNKVIVYAEEQKTSDDFNNVIADVQESFNAALDAAKEIAADPAASQDAVDAAWKALMT
EIHKLGFVKGDITSLEALVSLAEGYDMNDYVEAGQAEFLEALKTAQDLLADKDNAMQVEI
ETAESNLLNAMLNLRYKADKSILEKVIAEANGKDANAYTAESYAVLQAAVAEANAVMANE
DATQEEVDAAVERVQEAMKGLVAVEKPSTETPDDNKADGTQTGQESTITKANAAKTGDVA
PIAGIMVLALAGTAVTLLKKRK

Enzyme Prediction     

EC	3.2.1.40	3.1.1.73

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	542	1060	5.7e-179	0.9940476190476191
CBM67	343	517	1.8e-33	0.8863636363636364
CBM32	1215	1349	4.8e-16	0.8548387096774194

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	7.89e-134	648	994	1	340	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam08531	Bac_rhamnosid_N	4.83e-55	357	534	1	172	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam05592	Bac_rhamnosid	1.43e-42	545	644	4	102	Bacterial alpha-L-rhamnosidase concanavalin-like domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam17390	Bac_rhamnosid_C	1.98e-18	1004	1076	7	78	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam00754	F5_F8_type_C	5.91e-10	1211	1336	6	117	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SDT43572.1	0.0	38	1354	1	1301
SCG46613.1	3.93e-277	30	1104	31	1072
AVT31775.1	9.07e-268	8	1139	8	1110
SDT43619.1	1.69e-267	30	1135	42	1117
AVT37932.1	3.75e-266	8	1126	8	1097

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3W5M_A	1.54e-217	37	1109	3	1032	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
6I60_A	1.04e-150	26	1103	18	937	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]
6GSZ_A	4.44e-145	349	1066	134	851	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q82PP4	2.19e-216	37	1103	3	1026	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
T2KNB2	2.06e-168	46	1108	42	923	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
P9WF03	1.26e-130	328	1062	152	879	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
T2KPL4	8.10e-98	37	1105	29	947	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1
E8MGH9	8.46e-09	1282	1702	1577	1932	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001601	0.997456	0.000246	0.000277	0.000194	0.000188

TMHMM  Annotations     

start	end
12	34
1679	1698