CAZyme Information: MGYG000001560_00337

Basic Information

• Species: Massilioclostridium coli
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Massilioclostridium; Massilioclostridium coli
• CAZyme ID: MGYG000001560_00337
• CAZy Family: CBM67
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1678		183580.98	4.1159

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001560	2985330	Isolate	not provided	not provided

• Gene Location: Start: 397083; End: 402119 Strand: +

Full Sequence      

MNSKFVKKLAACIVASCLMATVAVPTVANAAGANYSVALTSIVDTTTNYRENPIGIDTDS
LRFGWRMESNLIGQQQAAYEINLYKASDSSKAIWTTGKVDDGVSTGILYNGEPLDATTGY
NWVVTVWDEQGKTYVSDPAYFETGVTNEQDWNAAEFIQLPVSSAAPIFRTEQTVDGTVDS
ARLYITAIGVYEAYINGEQVGKINKDGTTTYHHMNPGYGNGTTTIGYETYDVTSYLNDDA
PFALSILAGTGWNDGKGDGAMGSTSGQPAVKAMMVINYTDKDGNQQQKKVATNTEDWKGT
LDGPITVNGVFYGEDYDARKEAALGDYKNVGYDDSNWLSSGSKEQTDSHILTNTFAPVTT
KHLRLSVSETGPATSNDKENRLQIMELEALDTAGNNVVKGKLATVSSNQEVGDQWNVANM
TDGDDGTKTDYGYTSAILGTEGLEAFHSDPPLTVDFAFDQPTELASLKLYCRTGIASVTN
GVCPNYAKVYSIQVSDDGQNWRDVVTDYDAGRVENSFLQTTGITTTEYPGQIIASRGDSG
QIVDAFEHQPVSVTLYTGEAAESQYEGGEIAVDNYYAYQPSEDELYQNGFVQVPEGQPIF
ENGITLKKGQTMIVNIGQNMTAIPEISFQSVPGATVSMKFAEMLNDGSSVGNGAFQANGP
KGSLYTKSLRSARSEAHYTFATNAIETYQTKMSFFGYQYVQITATEDVTIYSMRSRALSS
ISEQTGHITTNNEDVNRLFLNALYGQLSNYFTIPTDCPQRDERKGWTGDAQVFAQTGIYN
FDAASFMIGYQDMLSELTMKQGYPGAVTSQWGWFNHWASGWSDVQVIYPWILYQQTGDRS
VLETNWEEMNHYMDWLKNNERASYQAPAIQRNGFGDWLAFQGTGYEVIADYYYGYVTQLM
EQMAGILGDAEKQSYYSERFENLKQTFLNTHVSFNDEPQLDPTIFEQPTTDNPGTSNIIT
NTFNETTAKYVRFTVSRTGPGTSDDNEYCLQMMELNVSKDGGENYALGKSVEASNTFDWG
TSWGNAYLTDGNTETGYSSVNNGGNTLTGNPITVTVDLGQEQTFNQIGITCRNYDKSMET
GVCVNYPKDYTIEVSSNGTDWTTIGRFATDNKPADKLVIKSGTGSPVMQNKGGKYEDNSQ
TALLWMLKLGYYTSDDMKDEAIRLLVKNIKNEDPDPSSIRAKYGENTLSVGFLGSNVITP
VLTDSGYSDVSYDLLLNDSMPSWLFEVKAGATTIWERWNSYDPENGFGDQEMNSFNHFSY
GSVAEWMYQYMVGIDSDIENPGFKHIILQPTLDTGEKYNDQNRINTVNGSYDSYYGLIES
NWTSDQNQLSTYHAVIPANTTSTLYLPVSADKMEGFTAISGVTFVGMTEHNGIEVAQFEL
VSGGYDFAVQNGKVVASIADGYVTEDAEPSDTDKTILDAVITYAENAKASGEYDNAIESV
QKSFDKALEEAKTIAADHNASQETINQAWKTLLNEIHKLGFVAGDKTTLASLIEAANGIN
AELDRYVETGKAEFTAALEAAQAVYQDGDAMQTEVNEAADNLLNAMLNLRYKADKSILEE
VLAEANKIDANAYTEESYAVLQTAIAEANEVMANENASQEEVNTAVANIRAAMDGLVAVE
GGTTETPSTKPTEEGVQTGQETTTTKANAAKTGDFAPIAGIATIAIAGMTLILSRKKK

Enzyme Prediction     

EC	3.2.1.40

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	607	932	4.6e-75	0.6091269841269841
GH78	1131	1346	2.3e-52	0.375
CBM67	162	339	2e-19	0.8579545454545454

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	4.63e-71	723	1271	1	340	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam08531	Bac_rhamnosid_N	1.60e-28	176	339	1	149	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam05592	Bac_rhamnosid	6.45e-15	604	709	1	97	Bacterial alpha-L-rhamnosidase concanavalin-like domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam17390	Bac_rhamnosid_C	1.99e-11	1273	1350	1	67	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam00754	F5_F8_type_C	1.30e-09	1010	1104	1	80	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACX65306.1	7.04e-177	42	1387	7	1004
AYA74416.1	5.77e-174	41	1401	34	996
AZQ32660.1	2.26e-167	40	1386	49	1035
QEV65837.1	3.93e-167	40	1385	49	1030
QKZ16767.1	5.46e-167	40	1385	49	1030

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3W5M_A	8.58e-57	42	1390	6	1031	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
6GSZ_A	1.03e-37	498	1347	205	847	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]
6I60_A	1.21e-36	611	1347	363	899	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q82PP4	5.74e-56	42	1389	6	1030	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
T2KNB2	9.82e-52	599	1347	358	879	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
P9WF03	9.75e-46	611	1323	367	856	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
T2KPL4	1.71e-36	48	337	38	344	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1
E8MGH9	2.38e-06	1408	1605	1662	1864	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.002422	0.995302	0.000536	0.000658	0.000526	0.000523

TMHMM  Annotations     

start	end
1655	1674