CAZyme Information: MGYG000001560_00526

Basic Information

• Species: Massilioclostridium coli
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Massilioclostridium; Massilioclostridium coli
• CAZyme ID: MGYG000001560_00526
• CAZy Family: CBM67
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2124		234431.3	4.1904

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001560	2985330	Isolate	not provided	not provided

• Gene Location: Start: 647006; End: 653380 Strand: +

Full Sequence      

MKKKNRILALILAVAMALSIVMPTNIFAAEQDTTIIEMKTNDLVDPMGIDTPNPVFSWKM
DSTITGQKQTAYQILVAKDKDLKESVWDSEKQETSQSVGIQYAGEPLENSTTYYWQVTVW
DKDGKSIASDVATFEVGLMGEDAWDGSRWIQMGTSTEPPEIVGKTNYTIEFDVQVDNTAV
GFLLEAKDTSNYLMWQFNISSGKMMLNPHTKIGGNYNGFKSIDISSGVKGEITDVQHVKL
EVNEDIGITTYLNGTKVDVTELSQLGGIGFNGTIGKIGFRTSSKEIGWLDNIVLTDYAED
ENGVVVKNYHFDNGENPFTAGSIQDGRLNTGLNADCLEKGEEQPPLDDKTHYTVDVDMTC
ETYAVSAIFNAVDQSNFYMWQLNTKDQEGKVILKPHTWKNGAYATYNSQNVDVTDAVGGV
ENFKTTPAHMHLDINGTRIETYINDTLVSTFMIGSTSDQSTTGIPVKAGYLGVRSSVTDG
VAEYGTVDNFKLTDYTDNEQGTVLYNYTFENGENPFGKGEIKDGAFVVTKVDILLPIEAQ
NNGAQTFRKQFNVKDDLVSAKLYTTGLGVYDVHLNGERVGQVMADGSVVYDELKPGYTAV
GDRVLYNTYDVTSMVQSGGDNTITANVTSGWWNGRIANSGRNNGFRAKMLLTYSDGHTEV
MGTDQTWRTAYAGPVISADIYEGETYDANADLSYRENGFDDSNWNYADICTAFRGEISAQ
AGDSVRVRTDLELHPKEITVYHGVVDENENQYGRINVTGNYQDGESFTLKAGETAVFDLG
QNFAGRPVIQVEGPKDTTVTIRHGEMLNDNDGLKSRGNDGPEGSIYTANLRTAPATAHYI
MNGNGVETYFSTHTFYGFRYVEVTATSEITIHNLKGEVLTSVKTDTGKLSTSDEDVNQLI
SNTVWGQYSNYLSVPTDCPQRDERQGWTADTQVFSTAAAYNAESKAFLQKWMRDMRDDQA
SNGAYPDTAPGGGSGQLGWSDAGIIVPYNMYKMYGDKTFIEENYTSMQKYIDVYLASTNK
KGAGHAYGDWLAYESNDDDMKSLLGIAFYAWDAQMMAEMAEVMGKPEDVAKYQQIYEIEK
EYFQQQYVNEDGSLKRGEQTACLYALYLDLLPNEDSFETVKQQLLDNISRNGNKLQTGFL
GTAIIMQTLSKIGADDVAYQLLLQHGNPSWLYSVDQGATTMWERWNSYTKETGFGDVSMN
SFNHYAYGAVAEWMYGYMAGILYDFDQPGFQHIILQPTPDQSLDFVNGEYNSAYGKIISN
WKYEGNTWSYEAVVPANTTATIYLPVEDGKPFTVNGKTPEDVTVETDGLAYKGMEDGKAV
FEAVAGSYSFQTEVTEYCYVTIGNADDTVPNQISVNGGIQQSLPLTIKATVGETITLEAK
PINDVDYQFVGWSGDVLSNESKITVTPTADMALTVSNTWIGNENLALNSKVDSNCSWTVS
TWAPEYLVDGILTSNGEHSGFTSGQTSTDMVDYWIELDLGADVEMNRIHLYPRSDVKSNS
GGTPSFPRDFSIQIKADGASEYTTVAEYTDYQAPQGKPAVFEFDLTNARYIRLRVTRLGD
PPAGETNYLQLAEMGVYHTTATSVDKTALENLYNSLKDTDLSQYKDGSAKDTFKAELEKA
ATLLGSDTATQDEINNAVTSLQAAFDALEKKPVDKHILQYVIEQAIAKKDTDEYKNVIPA
VKESYNKALEEAQAVYNDPNASQEEVNQAYAEMIEQIQNLNKQAGDKTELQALYDQVKDT
DLDQYLDGETKENFKTALEAAKEVLDDENALVKDVETAYNNLKASYEALEKKPIGEVDKT
ILEKVIAEANRLKETDEYNNAIPSVQQSFDKALEEAQNVYDNPSATQEEVNTAWQTLLKE
IHKLGFQKGDKTALQELYNQVKDTDLSQYRDGAAKENFKTALANAETVLADEEAMQKEID
KAYNDLKAAYEALEKLADKSQLKALLDECAGYQEEDYTPATWELFAGIQEKAQEVYDNAN
ATQEEINAAIDELLSGMLQLRFKADKSILETVIKVAGEIDGSSYTAESYGILQAAVAEAN
KVMADENASQEEVDAAATKVQEAMKGLVTVETPAENNHADGTQTGQESTTPKANAAKTGD
FAPIAGLLILAMATGTVIATKKRK

Enzyme Prediction     

EC	3.2.1.40	3.1.1.73

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	770	1284	2.2e-161	0.9900793650793651
CBM67	540	712	9.7e-27	0.8977272727272727

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	2.85e-124	885	1218	2	340	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam08531	Bac_rhamnosid_N	1.59e-44	556	728	2	171	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam05592	Bac_rhamnosid	9.26e-27	774	879	7	101	Bacterial alpha-L-rhamnosidase concanavalin-like domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam17390	Bac_rhamnosid_C	2.16e-18	1226	1289	5	68	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
COG0419	SbcC	6.86e-12	1568	2065	255	733	DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADD61991.1	5.20e-254	26	1330	12	1145
QUT40529.1	1.02e-253	26	1330	12	1145
BCA51920.1	1.42e-253	26	1330	12	1145
QQA09206.1	2.78e-253	26	1330	12	1145
QMW84969.1	7.62e-253	26	1330	12	1145

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3W5M_A	5.85e-138	46	1334	17	1032	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
6GSZ_A	2.72e-106	527	1300	115	862	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]
6I60_A	3.40e-95	544	1333	176	942	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KNB2	1.18e-140	545	1333	175	923	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
Q82PP4	3.20e-137	46	1330	17	1028	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
P9WF03	6.76e-104	534	1324	160	911	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
T2KPL4	8.72e-57	536	1313	176	935	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1
E8MGH9	1.23e-07	1870	2120	1667	1921	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000244	0.999055	0.000177	0.000184	0.000165	0.000148

TMHMM  Annotations     

start	end
7	29
2101	2120