CAZyme Information: MGYG000001571_02245

Basic Information

• Species: Lacrimispora sp000526575
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lacrimispora; Lacrimispora sp000526575
• CAZyme ID: MGYG000001571_02245
• CAZy Family: GH28
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1988	MGYG000001571_16|CGC2	217409.74	4.5432

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001571	4730558	MAG	United States	North America

• Gene Location: Start: 49653; End: 55619 Strand: +

Full Sequence      

MKVLRKRCKKALALFLTAALLLPFTVSGGQIAYAERFFEQDLENPATPSEAGRMLVPVTD
LEATPSEAIVSLLDNTATPSEASVSFDEIATPSQAMSLMGTQSRFTKSEGSVDVTELRVP
TLAYDDTSISLVWDKPENYETVANYNVYLNGIKVGDARSNFAQHADWAATYMKAFYDHYE
TVGIDMVKVDIHSFRLTGLTPDTSYEVSVIATDDSGAEMGNKATITCSTTSAPEIFDIRD
YGAEPVERGFTSLDEESSAFVVKNTKAIQAAIDDCSEGGKVVIPEGIYMSGALYLKSNMT
LELKEGAILFGSPDVAHYDQNYLLYPYSTDTRSWALINTYSADENGGFENIRIVGKGTIY
GNGWKYGAKQAVNGDGYETYYQKRQTADPDWSLEGLKKYALKGWVAQPKEKGDLSLGILA
KSAYNNAISKGYEESPAYSTRPNLVVLRNVNGVYIEGFTAENPANHTIAVLDSENVVSEN
VKYITYDANNADGIELGNTQNAMVFNNFFDTGDDGINFATGMGKGVQDTEQKPSSNIWTF
NNFLRECHGGAIAAGSHTGAGIENMLVEDNVMNHSDTPFRFKSAPANGGGIYNVSIRDCA
VGDADQVFSFNTTYSDANQATTVEPADKPAEFYDISAYNITADSINKLTIDILADVDYDL
PYKTWHNHHNLYFQDIVFTNIKPVKNSERIMGAANVTFDNVGLSWSKDAPAETDERAWGN
IKFSNDLIFTGTTTLSKNAYDAMAKPVWPDDTATVASASEAVKPSGAHEVSLKWPAAKDG
EQVSGTGKVTGYIVETYVGDELVDITRPVTTTTADMTGLSPDTSYVFVVYAVDGPGNKIP
GPRYEITTSTGIEKELKEPESKKVEFSNMGYTWATAKLKSAKAADARVRGYRAFVDGDLV
ATIYNYQLQEPEKDTISMTIGRMMENENEVIIEAFSDDGESFEYDNAAVKTIKNYDYHAP
VWESNALKASADGETIKLTWKAPTDESGIYGYRIYADGKPVYTKEGDYFNHVNGSFTTKD
TSYTVTGLDMKIPHTFRVEASDAWGKALTGTGPFHWTNSGPEITWDGSEGEQLWRSSAFG
QSTDMDFKSNILPNKVGTNYTWPIGSSTPLNLEGKPAQDVVIESRGGKLQTGHDGLTFYY
TEVPTSKNFSLTADVTVEQLGPEEGSKANLQEGAGLMVRDVNGSPRKEPLEEGYEEYPAA
SNMVMLSLQAAGKALNADINLLAAARYGVNSPAGNLKTVKTTATFEKSVSKADKKYAPAD
APFTSNFYGTETKLRLERTDEGFFVYHLDQQGNVLTSYTFEDKNVTPNIVSHLDKETMYV
GFFASRNARMSVRNISLDVSDVVKPDESPAYVAPDNDSASLYIASAAYTNSSDYTVQALT
NQSGTMTVSQNDTVLVSKASVTGGVQFTCPAIISGQEAEFKLSFVPSEGADAGKEKTTVL
KVKRDNYGKELYVSPEGTDEAAGTKENPLSIKEAINRLVPGGTIYMAEGTYHSFTIPMTS
SGNKDARKALTAQGAVKVTGSSKIFVLDADYWDVTGLDVDGQKIEGSRGVMIHGSHNVLK
DSLIHNTSSDAGLTITKSRGSRSLWPSYNLVENCEAFDNVDGSRINADGFASKSGSGDDN
RFLNCVSHDNADDGWDTYNTLADGPNGRTIIENCVAYNNGNNGFKLGGEGREVPHILRNS
VAFHNNLDGITDNFNPGELTIENNTSFDNARFNYIMRPSPYKKGVNGELTADGTVRNNVS
FRTAAFSGSLKKVYDDKISAKVIENNYLYQNGTNAVGQENFRSLNPADLFYRQDGRIQFG
DFLRPVKGSKIDKAGAGAKLPGIGVDTPDPVDPEPGEKPTPVKPDRDRDSDSDRDYGIKT
SSNETKGDWEMDQSGWRLKKADGTYPKEEWAKVNGAWYYFGQTGYMTTGWNQVQGVWYYM
ETSGAMKTDWVLTEGKWYYMNLDGSMATGWVLVNNNWYYLSQNGECLMNTVTPDGYRVGE
NGAWIKAK

Enzyme Prediction     

EC	4.2.2.9

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL9	1448	1811	7.3e-100	0.9866666666666667
GH28	275	681	1e-53	0.916923076923077

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5434	Pgu1	4.64e-65	119	598	3	392	Polygalacturonase [Carbohydrate transport and metabolism].
NF033840	PspC_relate_1	5.88e-30	1869	1986	512	648	PspC-related protein choline-binding protein 1. Members of this family share C-terminal homology to the choline-binding form of the pneumococcal surface antigen PspC, but not to its allelic LPXTG-anchored forms because they lack the choline-binding repeat region. Members of this family should not be confused with PspC itself, whose identity and function reflect regions N-terminal to the choline-binding region. See Iannelli, et al. (PMID: 11891047) for information about the different allelic forms of PspC.
COG5263	COG5263	1.93e-28	1869	1985	175	313	Glucan-binding domain (YG repeat) [Carbohydrate transport and metabolism].
NF033930	pneumo_PspA	4.44e-28	1890	1985	545	660	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.
NF033838	PspC_subgroup_1	1.93e-27	1821	1984	424	575	pneumococcal surface protein PspC, choline-binding form. The pneumococcal surface protein PspC, as described in Streptococcus pneumoniae, is a repetitive and highly variable protein, recognized by a conserved N-terminal domain and also by genomic location. This form, subgroup 1, has variable numbers of a choline-binding repeat in the C-terminal region, and is also known as choline-binding protein A. The other form, subgroup 2, is anchored covalently after cleavage by sortase at a C-terminal LPXTG site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SET92437.1	0.0	1	1986	1	2000
QOV20136.1	5.43e-238	105	1060	65	1067
AGB80387.1	1.26e-153	1073	1803	27	712
BAS43604.1	1.60e-153	1068	1803	23	720
QLT67346.1	1.60e-153	1068	1803	23	720

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2UVE_A	1.08e-101	95	688	18	576	Structureof Yersinia enterocolitica Family 28 Exopolygalacturonase [Yersinia enterocolitica],2UVE_B Structure of Yersinia enterocolitica Family 28 Exopolygalacturonase [Yersinia enterocolitica],2UVF_A Structure of Yersinia enterocolitica Family 28 Exopolygalacturonase in Complex with Digalaturonic Acid [Yersinia enterocolitica],2UVF_B Structure of Yersinia enterocolitica Family 28 Exopolygalacturonase in Complex with Digalaturonic Acid [Yersinia enterocolitica]
1RU4_A	7.88e-26	1430	1740	1	315	ChainA, Pectate lyase [Dickeya chrysanthemi]
5OLQ_A	5.78e-16	1448	1789	2	395	Rhamnogalacturonanlyase [Bacteroides thetaiotaomicron],5OLQ_B Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLQ_C Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLR_A Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLR_B Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLR_C Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLS_A Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron]
3JUR_A	1.06e-12	265	589	44	336	Thecrystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_B The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_C The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_D The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima]
5OLP_A	1.08e-12	236	569	45	324	Galacturonidase[Bacteroides thetaiotaomicron VPI-5482],5OLP_B Galacturonidase [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P22751	3.59e-143	1073	1787	33	709	Pectate disaccharide-lyase OS=Dickeya chrysanthemi OX=556 GN=pelX PE=1 SV=1
P15922	4.94e-99	116	701	35	578	Exo-poly-alpha-D-galacturonosidase OS=Dickeya chrysanthemi OX=556 GN=pehX PE=1 SV=1
P0C1A7	1.43e-25	1412	1740	8	340	Pectate lyase L OS=Dickeya dadantii (strain 3937) OX=198628 GN=pelL PE=1 SV=1
P0C1A6	3.46e-25	1412	1745	8	345	Pectate lyase L OS=Dickeya chrysanthemi OX=556 GN=pelL PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.003925	0.992980	0.002348	0.000303	0.000211	0.000194

TMHMM  Annotations     

start	end
12	34