CAZyme Information: MGYG000001597_01421

Basic Information

• Species: UBA866 sp900543295
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; UBA866; UBA866 sp900543295
• CAZyme ID: MGYG000001597_01421
• CAZy Family: GH85
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1404	MGYG000001597_78|CGC1	152517.78	4.0068

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001597	2473217	MAG	China	Asia

• Gene Location: Start: 2818; End: 7032 Strand: -

Full Sequence      

MKTSIGKRIGSGVLALALTATLYVGGSASVFAGDTYPYVGESAPGDNQPYLHGYRVEDLM
NWSPETDPYAELLRAQVPLQNRNEAFAATQANPDLTAAAQYLTLTGDYGNSFFNADAYTN
EFSTHIFNFWQYVDQYASWHGVPSVGTPEELNDVEDERNATDGNAWKRRNFEFGLVNLPN
PAYTNAAHKNGVLSLGCMFQPRAYQNFEVMLYQDENGRYPVADKLTEMAEYYGFDGYFFN
MEGRSYSASTREQLKSFFAQMRADGMYIQWYNAGSFSTDMLTSATKDNYEDATLYANSAF
IEYGHSVPGDEGTAPYGLDKFETAFNGFEAGANRWSNNFSRLMQDGVMNGSIASLGTDFV
QTGLEQKAGVDEATGYNLYTRELNEYQWMAFQRERLWWTGNNNNNTTVLNPGITDGTSAI
EARDFTGIADYIAERSVVNGDTFYTNFNTGHGLEYVVNGEVSSNSEWSNINIQDILPTWQ
WWFETEGTQLSAEFDYGSKWTKAYNGGEAGEFGFDLIGAYNGGSSLAVYGALDAENFMHL
YKSNLDVKETSKMEITFKKTSDDAASMKLGVIFADDTDTIVKLDIADSAAKSDEWVTSAV
DLSAYAGKKIAAFGLVFDGEAADYQMNIGQMKYTSGEAKAPAAPTGLAITKAYEDGEMTI
SWDMASYDEVKQYNVYAVKDGREIFLGGTYDENFYIKNVNEAIAEAEAVHVDSVTVSPKE
VDAVAGDTVDFDAKVNGYAEEAGQVTIVLKAVSADGTESEGAMTSHNYDEAVKNVTVDNS
EDGKLTLTWEGGEADVTVATSYEKESRTWTASGNNGCVVTVPTGAEANGAYVTVTVTTKS
GVSTMVDTTLPDHYCAPYDGKVYGSDGRLTQPMATDWHEIHYQVVTDGERADEVSVTRGV
GQGVTNDHAVFTPIAGSADGVYVWLVDYSGNVSEEVYVPNRLAVTVSSEGSTVQAGTTMQ
FTAVVKNFTESDEVTWSISGTQSSETTIDENGLLTVAEEESSTYMTVTATSKEDSTCSAS
KQVTIKPAYALMADADYAYKGETVNCAVYYKGEPLPTEDYEWSVSASWGSLSENTKIENG
VLTVGADESAYNVTVTAKKGELSYTLRLELADLYSLDPNYAQVAKGESQQFTVKNNKTDE
ALPGTDFTWSISSPYAQWYGDITSADTKVDANGLFTMGADEECSYITLTATHKTTGAKYS
TSISQPWGYSLRAASAEETEETPVSAPAPTNNDPTVSQEVVWTVEGANSADTTVDENGTL
TIGIDETAEQLYVTATSAADETKSDTAVVNVTPREKFYVEVIAGEHGTVTPGSDEYYEGT
DVTFTFTPDKGYAVDKVTIDGEEVALTDGKYTLTVTGTAQIEVSFKVNPDAPDTGDYTPI
LAAALLAMLAAAGAAVYTIRRRRA

Enzyme Prediction     

No EC number prediction in MGYG000001597_01421.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH85	109	455	5.7e-48	0.9777777777777777

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4724	COG4724	2.17e-80	47	632	27	548	Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism].
cd06547	GH85_ENGase	5.39e-45	119	487	19	339	Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model.
pfam03644	Glyco_hydro_85	6.83e-45	119	452	2	291	Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates.
COG2373	YfaS	7.46e-04	928	1112	484	727	Uncharacterized conserved protein YfaS, alpha-2-macroglobulin family [General function prediction only].
pfam18998	Flg_new_2	0.003	1299	1332	3	35	Divergent InlB B-repeat domain. This family of domains are found in bacterial cell surface proteins. They are often found in tandem array. This domain is closely related to pfam09479.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNO18499.1	1.08e-308	14	1193	21	1170
AZH30533.1	3.60e-203	15	881	15	855
AUS27834.1	4.18e-201	15	881	15	855
AHM67228.1	4.18e-201	15	881	15	855
AIY08018.1	4.18e-201	15	881	15	855

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2W91_A	8.00e-47	57	777	18	643	Structureof a Streptococcus pneumoniae family 85 glycoside hydrolase, Endo-D. [Streptococcus pneumoniae TIGR4],2W92_A Structure of a Streptococcus pneumoniae family 85 glycoside hydrolase, Endo-D, in complex with NAG-thiazoline. [Streptococcus pneumoniae TIGR4]
3GDB_A	9.95e-46	57	777	169	794	Crystalstructure of Spr0440 glycoside hydrolase domain, Endo-D from Streptococcus pneumoniae R6 [Streptococcus pneumoniae R6]
3FHA_A	1.53e-41	47	715	4	603	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_C Chain C, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
3FHQ_A	1.53e-41	47	715	4	603	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_F Chain F, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
2VTF_A	9.41e-41	47	715	9	608	X-raycrystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae],2VTF_B X-ray crystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000761	0.998364	0.000289	0.000219	0.000182	0.000162

TMHMM  Annotations     

start	end
9	31
1377	1399