CAZyme Information: MGYG000001599_02103

Basic Information

• Species: Phocaeicola sp900544075
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Phocaeicola; Phocaeicola sp900544075
• CAZyme ID: MGYG000001599_02103
• CAZy Family: GH115
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
843		97012.46	5.2464

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001599	4213385	MAG	China	Asia

• Gene Location: Start: 69862; End: 72393 Strand: +

Full Sequence      

MKKPYILIVLLFISVFSNSANQFISFKRVDGSLQILKNGKVVNIIYDNSDQKGISIAINN
LVEDFNRVCGVKPELLDEVKDKNCIIIGSIESKYIRQLIKSKKLDKKELQGKSEKYIITT
VDNPLEGVDKALVIAGSDRRGTIYGIYELSEQMGVSPWYWWMDVPVEKHTEIYAMPGVYT
DGEPAVKYRGIFLNDEAPCLTSWVNNHYGTDFGDHRFYADVFELILRLKGNFLWPAMWGW
AFYADDPLNSKTADEMGVIISTSHHEPMARNHQEWARKRKEYGAWNYSTNKKVIDQFFRE
GIERVKDTEDIITIGMRGDGDEAMSEDTNVKLLESVVENQRKIIKEVTGKPAKETPQLWA
LYKEVLDYYDKGMRVPEDVIMLLCDDNWGNVRRLPNEKERLHPGGWGMYYHVDYVGAPRN
SKWMNMTPIQGMWEQLHLTYEYGVDRLWVLNVGDLKPMEYPIEFFLDMAWNPDAYTAENF
MEHARKFCVQSFGESQADEAARILNLYTKYNGRVTAEMLDAKTYNLETGEWKQVADDYAR
LEMEALRQYMSLSPEYRDAYKQLLLYPVQAMSNLYEMYYAQAMNHKLYADGNPMANLWAD
KVEKCFARDKALSDDYNNVMAEGKWKGMMIQKHIGYTSWNDNFPADKLPEVKRIAEAEKA
VGGYVFTGKNGYVAMEAPHFYENNAPEGTEWKIIPDMGRTLGGVTLMPYTKSAEGASLSY
KMQLPEGVKDVKVIVVVKSTLAFADVKGHEYTVGFRGGNTETVNFNHNMNEDKENVYTVF
YPTVARRVIEKEVNLELPVSEDSTYVLDFKPLHPAVVLEKIVVDYGGYSKSYLYMNESDC
KRN

Enzyme Prediction     

EC	3.2.1.131

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH115	32	821	2.9e-281	0.9956958393113343

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam15979	Glyco_hydro_115	0.0	182	518	1	334	Glycosyl hydrolase family 115. Glyco_hydro_115 is a family of glycoside hydrolases likely to have the activity of xylan a-1,2-glucuronidase, EC:3.2.1.131, or a-(4-O-methyl)-glucuronidase EC:3.2.1.-.
pfam17829	GH115_C	1.99e-29	665	833	1	168	Gylcosyl hydrolase family 115 C-terminal domain. This domain is found at the C-terminus of glycosyl hydrolase family 115 proteins. This domain has a beta-sandwich fold.
pfam03648	Glyco_hydro_67N	6.86e-06	54	149	33	120	Glycosyl hydrolase family 67 N-terminus. Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. This family represents the N-terminal region of alpha-glucuronidase. The N-terminal domain forms a two-layer sandwich, each layer being formed by a beta sheet of five strands. A further two helices form part of the interface with the central, catalytic, module (pfam07488).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
EDY95690.1	0.0	1	842	1	843
QUR44901.1	0.0	1	843	1	843
QUT26893.1	0.0	1	843	1	843
QDH54071.1	0.0	1	843	1	843
CBK67960.1	0.0	1	843	12	854

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4C90_A	0.0	1	842	12	853	Evidencethat GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C90_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_A Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus],4C91_B Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility [Bacteroides ovatus]
7PUG_A	0.0	35	842	19	838	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
7PXQ_A	0.0	35	842	18	837	ChainA, xylan alpha-1,2-glucuronidase [uncultured bacterium]
4ZMH_A	7.18e-196	23	718	5	696	Crystalstructure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40],4ZMH_B Crystal structure of a five-domain GH115 alpha-Glucuronidase from the Marine Bacterium Saccharophagus degradans 2-40T [Saccharophagus degradans 2-40]
6NPS_A	4.98e-125	53	642	31	648	Crystalstructure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112],6NPS_B Crystal structure of GH115 enzyme AxyAgu115A from Amphibacillus xylanus [Amphibacillus xylanus NBRC 15112]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000631	0.998626	0.000190	0.000193	0.000172	0.000180

TMHMM  Annotations     

start	end
7	26