CAZyme Information: MGYG000001602_00537

Basic Information

• Species: Blautia sp900547685
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia; Blautia sp900547685
• CAZyme ID: MGYG000001602_00537
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
634		73586.38	6.7643

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001602	2582611	MAG	China	Asia

• Gene Location: Start: 34827; End: 36731 Strand: +

Full Sequence      

MGVNMKITELDRYLFGEGTHYEIYKLLGAHVGVNRKKEGVFFAVWAPHAKSVSVVGNFNN
WDAKADIMKCDNNMGLYQLFIPKVREGDLYKFCITAQSGELLYKADPYANYSELRPGNAS
RVVDLTGYKWSDSVWMKKRQEFDVDKDAMSVYEVHPGSWKKHPAQGEDDPGFYNYRELAH
ELAKYVKEMGYTHVELMGIAEHPFDGSWGYQVTGYYAPTSRYGTPEDFRYFVNYLHKNKI
GVILDWVPAHFPKDAHGLADFDGAPTYEYADPRKGEHPDWGTKVFDYGKNEVKNFLIANA
LFWIEEFHIDGLRVDAVASMLYLDYGRRDGEWVPNQYGENKNLEAIEFFRHLNSVVLGKH
KGVVMIAEESTAWPMVTGKPEDGGLGFSLKWNMGWMHDFLEYMKLDPYFRKYNHHKMTFS
TSYAYSERYVLVLSHDEVVHLKCSMINKMPGLGQDKFENLKAGYSYMIGHPGKKLLFMGQ
DFGQYQEWSEERELDWYLLGEQNHQQLQNYVKELLKMYKKYPALYANDSNYQGLEWINAD
DASRSIYSFVRWSPTGRNNLLFVINYTPVARDDYRVGVPKRKQYKLILNSKEPEFGGDVH
KEVVDYKAVKKECDGRPFSFAYPLPAYGVAVFRF

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	185	484	1.2e-154	0.9933554817275747
CBM48	27	111	4.5e-17	0.881578947368421

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	8	634	8	627	1,4-alpha-glucan branching protein GlgB.
COG0296	GlgB	0.0	5	633	4	627	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK14705	PRK14705	0.0	7	632	603	1220	glycogen branching enzyme; Provisional
PRK14706	PRK14706	0.0	26	634	26	621	glycogen branching enzyme; Provisional
PRK05402	PRK05402	0.0	7	633	100	722	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASM70613.1	0.0	1	634	1	634
AWY99231.1	0.0	1	634	1	634
QIB55783.1	0.0	2	634	4	636
QMW76350.1	0.0	2	634	4	636
ASU30768.1	0.0	5	634	7	636

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GR2_A	3.79e-233	7	633	129	772	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQW_A	3.79e-233	7	633	129	772	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	5.36e-233	7	633	129	772	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A	7.60e-233	7	633	129	772	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	1.08e-232	7	633	129	772	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P30539	1.95e-296	1	634	1	632	1,4-alpha-glucan branching enzyme GlgB OS=Butyrivibrio fibrisolvens OX=831 GN=glgB PE=1 SV=1
Q8DLB8	1.88e-239	6	597	105	713	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
A1U0K1	1.38e-238	7	633	6	628	1,4-alpha-glucan branching enzyme GlgB OS=Marinobacter nauticus (strain ATCC 700491 / DSM 11845 / VT8) OX=351348 GN=glgB PE=3 SV=1
Q7NL20	1.86e-236	7	633	103	727	1,4-alpha-glucan branching enzyme GlgB OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=glgB PE=3 SV=1
B3PGN4	1.40e-234	6	597	114	699	1,4-alpha-glucan branching enzyme GlgB OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000050	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001602_00537.