CAZyme Information: MGYG000001607_00824

Basic Information

• Species: Dorea_B phocaeensis
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Dorea_B; Dorea_B phocaeensis
• CAZyme ID: MGYG000001607_00824
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1690		186258.96	4.7028

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001607	2347571	MAG	China	Asia

• Gene Location: Start: 13646; End: 18718 Strand: -

Full Sequence      

MKRRAVALIMALLLSGGLFNPVGFMEAKAAPAVESGGEKAQASQRNVMNFNTDWHYKKGD
VTGGEKVDFVDDQWVYVNLPHSTTFYTAENKDAYLGISWYRKDFQVDNSLKGKKLMLTFE
AAMQKADVYVNGKKIMTHEGGYIPFVIDLTDKVNYGAENTIAVKIDSRHNRNFAPGKDKP
DFQYFGGIYGNAYLTVTEDIHITDAVEAKETAGGGVFITSPKVAKEQATVKVKTHVENEG
SANKETTLLTEIVDAGGAVAASKEDKISIAGGSSTYFEQSLVVNQPRLWSPDTPELYQVR
STVKVDGVVKDVLETTYGIRKVEWKRDGLYVNGKKTDAEGANLHAETYMLGNAMPDNAIY
EEIKRFKEDGFDIVRMSHYPHRQAYYDACDKYGVMVVECASGWQYFSDSQAFKDSTYNEL
RTDIRHKRNHPSIVAWESSLNESSYNKAWATEMNRIVKEEYPKDGAAYGYTAGCYHWSVW
DIGLSTPQAGIFGTGNEGAENIKYKDKPMIIAEYGDWSYGGTNSSTRVTREDINTAGKKG
GDEGMLIQSNNIQESVQTNRSRGEKWLGASMYWDYADYAGFDAGMLTYCGVVDVHRLPKH
SAYFYQSQRAASVDMSKYGIATGPMVYIANTWDSKAAKEVRIFSNCDTVELFLNGVSVGE
KGHDQTTWGPHGDLDPSKHPSENSGKEIPTDAMKNAPITFNLEQYEAGELKAVGKIGGNK
VAEYVRKSPEAATQINLRPESDASVPLDGSSAKLVWIDITDQNGTVVPSAYQDVKLEVEG
PGLVVGPKTITTKGGQLAVWVRSKRGQGDITLRATAEGLNGASVTIPTSQVEGLPAVVEG
GDADEYEYNLAQEELQGKNIFLNKSSRASTENIANGKNERKENGNDGNDNTKWCASDGSY
PQWWEVDLGAMYQLDTMKLSFETEGSAYHYVIAVSNNPMTDENYANHIVVDNRQGSTDTE
LVFEKGQVQGRYVRVLFTEATNNEWAVLRDVSGTGETNNIALNKPVQASTVNTDSNGKLE
KAEYAVDGKKDTWWCAQGGVDTKDHWIQVDLKNTYKLSEVKLFFEKEDAAYKFVVQSSTD
GVHYKDIKDFRTGDGCGKEVTIPTDEYARYLRVYDIKTKNMRNQWPTVCELEAYGAKVDY
TLSSVTREKEAFASSCKEDVEAGHGSNGVPGWYWYPATLKDEWWYIDTKGIYDIDNVQMT
WNAEETHSYILDISTDGKNWTTVADRSKDGTNEIRPYEDVKETARYIRVRLPEGRTSEQG
FGLFDAYAPVPTGRRVKEVKENTKVTAFTGTEFAQLQLPKEVDVVLADGIKTTLPVVWNE
AGYDPAKEGEVTLTGTLTEIAGVKLAEEQKATAVIVALTKATEDPTITLQPQSQTAQVGG
NVVFTVEANSNDGGTLSYQWQENSGADWTDIEGATTASYQKENVQLEDNGKKFRCIVTNT
KDGAEAKQIITEEATLTVEEEIKVNEIVSVETLPGKEVDFGTSESELDLPKEVKVQLDNK
EEKTLYVKWDTSTYNGNVANTYRLSGELVLTDAIANTAGVKAFIDVTVKEAAVKPEVDKE
ALEAIIAKGKEEAASGEYTSESVAALNEALAKAWEVVEKKDATEEEVKQAIQSVEQAINN
LEKKDSEGNLNGETGGPSGNPQGNTGDHSGSAQNSGAKVPKTPKTGDQGHALLWFAGMLG
AVAVIFRKRR

Enzyme Prediction     

No EC number prediction in MGYG000001607_00824.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	37	640	1.3e-92	0.6595744680851063
CBM32	1007	1125	1.4e-20	0.9032258064516129

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.18e-47	91	536	59	534	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	4.37e-29	94	442	63	417	beta-D-glucuronidase; Provisional
PRK10340	ebgA	1.12e-28	100	445	113	452	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	2.48e-19	227	445	238	465	beta-galactosidase.
pfam18565	Glyco_hydro2_C5	3.80e-16	748	825	13	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANN35851.1	1.28e-147	45	1095	18	1047
AWG23272.1	6.18e-145	41	827	22	779
SDT96238.1	1.69e-144	50	820	32	774
QBG46998.1	6.05e-144	48	821	28	774
AUS04893.1	2.61e-142	48	814	30	766

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5T98_A	9.44e-70	42	826	22	818	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
4YPJ_A	2.01e-68	48	828	10	807	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
7CWD_A	5.56e-67	48	829	4	802	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
6QUB_A	2.16e-64	42	833	27	881	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum],6QUC_A Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUC_B Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUD_A 2-deoxy-galactose reaction intermediate of a Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum]
6QUB_B	6.45e-64	42	831	4	856	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P77989	9.26e-93	45	831	3	741	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KN75	1.06e-59	38	832	19	799	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
P26257	4.44e-58	45	795	2	715	Beta-galactosidase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=lacZ PE=1 SV=1
A7LXS9	1.21e-56	43	831	40	851	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KPJ7	5.30e-51	35	664	41	678	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001387	0.996632	0.000522	0.000832	0.000361	0.000243

TMHMM  Annotations     

start	end
5	24
1669	1686