CAZyme Information: MGYG000001614_00354

Basic Information

• Species: CAG-521 sp002329575
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Burkholderiales; Burkholderiaceae; CAG-521; CAG-521 sp002329575
• CAZyme ID: MGYG000001614_00354
• CAZy Family: GH102
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
392	MGYG000001614_4|CGC1	43523.71	9.0445

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001614	1536892	MAG	China	Asia

• Gene Location: Start: 1840; End: 3018 Strand: -

Full Sequence      

MKISRLCFYVAVATMLAACGTPEPGKVPEVEPGQTKLTFEKTDFLSMPLVADWKPALNAF
KKSCTKMASRPGWSDVCAKASVVSDESAEAFFKSYFTPYKAIAISRSQGQGATTSMTDVG
KMTGYYEPILYGSRVKKAPYIYPLHTMPADLITVDLADVYPQLKGMRLRGQLEGNRLVPY
DTRAQLNNRNLDRYAIAWVEDPVAAFFLQIQGSGRIVLPDGSYMRVGYGDVNGHPYRGIG
NYLIRQGWLDAHELSMQNIQAWARKNPKRVQSVLNQNPSYVFFVERKDQDPAEGPIGAQG
VPLTDKGSVAVDRRFYEMGWPLVVDVEQTNPDMKYTAAVVTQDTGGAIRGPIRFDFYWGS
GDDAALAAGRQNSRVKAWVLFPQGVEPREKSI

Enzyme Prediction     

No EC number prediction in MGYG000001614_00354.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH102	131	283	1.8e-57	0.9808917197452229

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam03562	MltA	1.48e-99	51	283	4	230	MltA specific insert domain. This beta barrel domain is found inserted in the MltA a murein degrading transglycosylase enzyme. This domain may be involved in peptidoglycan binding.
COG2821	MltA	3.41e-89	1	388	4	371	Membrane-bound lytic murein transglycosylase [Cell wall/membrane/envelope biogenesis].
cd14668	mlta_B	3.36e-80	130	286	1	159	Domain B insert of mltA_like lytic transglycosylases. Escherichia coli MltA is a membrane-bound lytic transglycosylase comprised of two domains separated by a large groove, where the peptidoglycan strand binds. Domain A is made up of an N-terminal and a C-terminal portion, which correspond to the 3D domain, named for 3 conserved aspartate residues. Domain B is inserted within the linear sequence of domain A. MltA is distinct from other bacterial lytic transglycosylases (LTs), which are similar to each other. Escherichia coli peptidoglycan lytic transglycosylase (LT) initiates cell wall recycling in response to damage, during bacterial fission, and cleaves peptidoglycan (PG) to create functional spaces in its wall. PG chains (also known as murein), the major components of the bacterial cell wall, are comprised of alternating beta-1-4-linked N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), and lytic transglycosylases cleave this beta-1-4 bond. Typically, peptidoglycan lytic transglycosylases (LT) are exolytic, releasing Metabolite 1 (GlcNAc-anhMurNAc-L-Ala-D-Glu-m-Dap-D-Ala-D-Ala) from the ends of the PG strands. In contrast, MltE is endolytic , cleaving in the middle of PG strands, with further processing to Metabolite 1 accomplished by other LTs. In E. coli, there are six membrane-bound LTs: MltA-MltF and soluble Slt70. Slt35 is a soluble fragment cleaved from MltB. Bacterial LTs are classified in 4 families: Family 1 includes slt70 MltC-MltF, Family 2 includes MltA, Family 3 includes MltB, and Family 4 of bacteriophage origin. While most of the LT family members are similar in structure and sequence with a lysozyme-like fold, Family 2 (including mltA) is distinct.
smart00925	MltA	2.98e-73	129	283	1	152	MltA specific insert domain. This beta barrel domain is found inserted in the MltA a murein degrading transglycosylase enzyme. This domain may be involved in peptidoglycan binding.
PRK11162	mltA	1.73e-49	121	380	117	354	murein transglycosylase A; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBF22454.1	1.40e-148	11	389	12	392
ANU66434.1	5.14e-135	3	389	5	398
QQQ97583.1	5.14e-135	3	389	5	398
QDA55021.1	3.47e-131	11	389	24	393
QQS89395.1	1.97e-128	32	389	40	395

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7ESJ_A	3.63e-82	19	385	2	362	ChainA, membrane-bound lytic murein transglycosylase A [Acinetobacter baumannii],7ESJ_B Chain B, membrane-bound lytic murein transglycosylase A [Acinetobacter baumannii]
6QK4_B	3.72e-77	59	382	47	346	Lytictransglycosylase, LtgG, of Burkholderia pseudomallei. [Burkholderia pseudomallei]
2G5D_A	1.73e-75	45	387	48	418	Crystalstructure of MltA from Neisseria gonorrhoeae Monoclinic form [Neisseria gonorrhoeae FA 1090]
2G6G_A	2.31e-72	52	387	57	418	Crystalstructure of MltA from Neisseria gonorrhoeae [Neisseria gonorrhoeae FA 1090]
3CZB_A	2.85e-45	52	382	22	341	Crystalstructure of putative transglycosylase from Caulobacter crescentus [Caulobacter vibrioides CB15],3CZB_B Crystal structure of putative transglycosylase from Caulobacter crescentus [Caulobacter vibrioides CB15]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8K9A7	5.39e-27	121	380	125	364	Membrane-bound lytic murein transglycosylase A homolog OS=Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) OX=198804 GN=mltA PE=3 SV=1
P57531	4.17e-25	121	380	117	356	Membrane-bound lytic murein transglycosylase A homolog OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) OX=107806 GN=mltA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000000	0.000000	1.000077	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001614_00354.