CAZyme Information: MGYG000001622_00282

Basic Information

• Species: Enterocloster sp900549235
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Enterocloster; Enterocloster sp900549235
• CAZyme ID: MGYG000001622_00282
• CAZy Family: GH2
• CAZyme Description: Beta-glucuronidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
602		69292.19	5.7253

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001622	3555335	MAG	China	Asia

• Gene Location: Start: 280493; End: 282301 Strand: +

Full Sequence      

MLYPQINGSRMMYDLGGVWDFFLGDTKEPGVCFEMGDRKPEPIAVPASYNDQKDDPAYRN
HYGWVYYYREFRVPFMLLGQRLVLRFDAVTHWGKVYVNGRLAAEHKGGFLPFEIEVTHLV
KAGETCRLLVAVDNRVDHSTLPVGNEGATAFFGSDNPGVPGVEAAKRWRKPQNMPNFDFF
NYAGINRPVRLYTTPAAYIQDITLVPGIQGTEGLVDYEIKTGGPAEEEPVRIRILDAEGN
LAAEGEGRKGRLNISDARLWWPWPGEPYLYRAMVTYGKDTYEQSFGIRTVKVDGTRFLIN
GKPFYFKGFGKHEDSAFHGRGLDLCLDVKDVNLIHWLHANSFRTSHYPYAEEMYDLCDRE
GIVIIDETPAVGIGAGADCDPYKTFRIREHHEQVLRDMIARDKNHPCVVMWSMGNEPDTE
HFPESAYDYWHSLYELTHRLDPADRPVTFVCCQNNYEKDLVTRTMDVVCINRYYGWYNLS
GDLDAACYGLNQELDFWEKQGKPVMITEYGADAIAGIHQCVPEMFSEEFQMEYYRRQNQE
FDKRSFFIGEQVWNFADFAAVQGCMRADGNKKGLFTRDRRPKLAAHYFRKRWGEVPDFGY
KG

Enzyme Prediction     

EC	3.2.1.31

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	9	590	3.4e-105	0.6090425531914894

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10150	PRK10150	0.0	1	601	1	601	beta-D-glucuronidase; Provisional
COG3250	LacZ	3.52e-103	9	592	9	595	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam02836	Glyco_hydro_2_C	3.93e-78	290	595	1	300	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10340	ebgA	1.13e-32	67	476	113	492	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02837	Glyco_hydro_2_N	7.20e-24	15	195	4	169	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CUO31707.1	0.0	1	601	1	600
AEN95939.1	0.0	1	601	6	605
CBL12719.1	0.0	1	601	1	599
VCV23245.1	0.0	1	602	1	600
CBL08206.1	0.0	1	602	1	600

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6U7I_A	4.15e-301	1	601	1	597	Faecalibacteriumprausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_B Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_C Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_D Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii]
6LD0_A	5.96e-254	1	602	3	670	ChainA, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD0_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD0_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD0_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD6_A Chain A, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD6_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD6_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD6_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDB_A Chain A, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDB_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDB_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDB_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDC_A Chain A, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDC_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDC_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDC_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDD_A Chain A, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDD_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDD_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDD_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1]
5Z1B_A	1.04e-252	1	602	25	692	Structureof Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide [Bifidobacterium dentium ATCC 27679],5Z1B_B Structure of Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide [Bifidobacterium dentium ATCC 27679],5Z1B_C Structure of Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide [Bifidobacterium dentium ATCC 27679],5Z1B_D Structure of Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide [Bifidobacterium dentium ATCC 27679]
6BO6_A	1.95e-215	1	601	1	609	Eubacteriumeligens beta-glucuronidase bound to UNC4917 glucuronic acid conjugate [[Eubacterium] eligens ATCC 27750]
6BJQ_A	4.28e-215	1	601	25	633	ChainA, Glycoside Hydrolase Family 2 candidate b-glucuronidase [[Eubacterium] eligens ATCC 27750]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P05804	1.07e-162	1	601	1	602	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
O97524	4.52e-154	1	595	27	628	Beta-glucuronidase OS=Felis catus OX=9685 GN=GUSB PE=1 SV=1
Q4FAT7	3.71e-153	1	595	28	629	Beta-glucuronidase OS=Sus scrofa OX=9823 GN=GUSB PE=3 SV=1
O18835	1.44e-152	1	595	27	628	Beta-glucuronidase OS=Canis lupus familiaris OX=9615 GN=GUSB PE=1 SV=1
P12265	1.86e-146	1	595	27	625	Beta-glucuronidase OS=Mus musculus OX=10090 GN=Gusb PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000034	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001622_00282.