CAZyme Information: MGYG000001641_02140

Basic Information

• Species: CAG-485 sp900760815
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-485; CAG-485 sp900760815
• CAZyme ID: MGYG000001641_02140
• CAZy Family: GT2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
456	MGYG000001641_57|CGC1	51750.04	4.4769

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001641	2823092	MAG	United States	North America

• Gene Location: Start: 3586; End: 4956 Strand: +

Full Sequence      

MELIRFKTVNELRERIFRTPLEQYVLLQLDDRNIQPDDNCFHRLLQVAEEVDSTLTYCWF
RDRLPDGTIRNHPVNDYQPGSVRDDFDFGPMVLLNAADVLAATEDFTDEESSMIDGGWYA
MRLRLTASNMIAMIQEYLYTAAEPDLRDSGAKQHDYVNPRNRAYQIEMEKALTDHLYEIN
GLVSPQKETVDYDSEIFPAEASVVIPVRNRARTIMDAVHSALSQKTDFPMNVIVVDNEST
DGTRELLEAVTDPRLRLIKVAASENLGIGGCWNRALLSEHCGRFAIQLDSDDVYNSENVV
QTIVDKFRAGNYGMVIGSYTLTDFDGNVIPPGVISHSEWTDTNGPNNALRINGLGAPRAI
FTPLGRQFLFPNVSYGEDYAMTLRISRDYAIGRIFQPLYNCRRWEGNSDAALSIEKINAN
NDYKDCLRSIELTARVKQNQEYLRDQAAGIVDNLPF

Enzyme Prediction     

No EC number prediction in MGYG000001641_02140.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	202	332	6.5e-20	0.7529411764705882

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00535	Glycos_transf_2	5.15e-18	202	331	1	127	Glycosyl transferase family 2. Diverse family, transferring sugar from UDP-glucose, UDP-N-acetyl- galactosamine, GDP-mannose or CDP-abequose, to a range of substrates including cellulose, dolichol phosphate and teichoic acids.
COG0463	WcaA	3.23e-16	202	316	6	116	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].
cd00761	Glyco_tranf_GTA_type	2.03e-15	203	321	1	117	Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold. Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities.
cd06433	GT_2_WfgS_like	7.86e-13	202	391	1	182	WfgS and WfeV are involved in O-antigen biosynthesis. Escherichia coli WfgS and Shigella dysenteriae WfeV are glycosyltransferase 2 family enzymes involved in O-antigen biosynthesis. GT-2 enzymes have GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.
cd04179	DPM_DPG-synthase_like	2.18e-12	203	318	1	115	DPM_DPG-synthase_like is a member of the Glycosyltransferase 2 superfamily. DPM1 is the catalytic subunit of eukaryotic dolichol-phosphate mannose (DPM) synthase. DPM synthase is required for synthesis of the glycosylphosphatidylinositol (GPI) anchor, N-glycan precursor, protein O-mannose, and C-mannose. In higher eukaryotes,the enzyme has three subunits, DPM1, DPM2 and DPM3. DPM is synthesized from dolichol phosphate and GDP-Man on the cytosolic surface of the ER membrane by DPM synthase and then is flipped onto the luminal side and used as a donor substrate. In lower eukaryotes, such as Saccharomyces cerevisiae and Trypanosoma brucei, DPM synthase consists of a single component (Dpm1p and TbDpm1, respectively) that possesses one predicted transmembrane region near the C terminus for anchoring to the ER membrane. In contrast, the Dpm1 homologues of higher eukaryotes, namely fission yeast, fungi, and animals, have no transmembrane region, suggesting the existence of adapter molecules for membrane anchoring. This family also includes bacteria and archaea DPM1_like enzymes. However, the enzyme structure and mechanism of function are not well understood. The UDP-glucose:dolichyl-phosphate glucosyltransferase (DPG_synthase) is a transmembrane-bound enzyme of the endoplasmic reticulum involved in protein N-linked glycosylation. This enzyme catalyzes the transfer of glucose from UDP-glucose to dolichyl phosphate. This protein family belongs to Glycosyltransferase 2 superfamily.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUB84322.1	3.21e-150	24	441	77	492
QUB51403.1	4.76e-146	15	441	69	494
QCD38257.1	6.14e-146	7	441	8	443
QUB69156.1	9.26e-146	16	452	69	499
QCD42299.1	1.32e-145	25	437	27	445

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P33697	1.29e-08	200	326	11	132	Succinoglycan biosynthesis protein ExoO OS=Rhizobium meliloti (strain 1021) OX=266834 GN=exoO PE=3 SV=2
P33700	3.84e-06	198	401	6	213	Succinoglycan biosynthesis protein ExoU OS=Rhizobium meliloti (strain 1021) OX=266834 GN=exoU PE=3 SV=1
O32268	5.54e-06	202	299	9	104	Putative teichuronic acid biosynthesis glycosyltransferase TuaG OS=Bacillus subtilis (strain 168) OX=224308 GN=tuaG PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000066	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001641_02140.