CAZyme Information: MGYG000001649_01156

Basic Information

• Species: UBA1409 sp900760125
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; UBA1409; UBA1409 sp900760125
• CAZyme ID: MGYG000001649_01156
• CAZy Family: GT35
• CAZyme Description: Maltodextrin phosphorylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
801	MGYG000001649_27|CGC1	90501.05	6.396

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001649	2605663	MAG	United States	North America

• Gene Location: Start: 1315; End: 3720 Strand: -

Full Sequence      

MAKAVESKYTKESLKEELVKVLDLNFMVSPENASDRQFYEALSSIIVNELVRKRHNYTTQ
VHSEGKKQVYYISMEFLMGRSLKTSLYNLGLVKAAEGALKDFGVNINSLYEQEPDAGLGN
GGLGRLAACYLDGLATDGYPATGYSILYEYGIFCQKLEGGWQVELPDNWLPGGGCWLVEH
PESQIEIHFDGKIKEYWDSHYHHVSLEDYTTVIAVPYDMFVSGYNSNAVSKLRLWKAISP
AFDMNKFNSGNYEKALSQNIVAQAISKVLYPNDNHLEGKSLRLRQQYFMCAASVGDIVNH
HMATYGTLDNFADKVAIQINDTHPTLAIPELMRVLLDDCGYPWDKAWDIVTRTFAYTNHT
VMAEALEKWSCDLLNTVVPRIYSIIVEINNRYCASLWEKYHDHQKVTGMSIIRDNVVHMA
KLCVCASHHVNGVSAIHSEIIKRDVFRDEYELTPEKFTNVTNGIAYRRWLYQSNPELCKL
LNDTIGKSYLDDAKNLKKFEKYADDAEVLARLGDIKRANKHSFAKYLKTNRGVIVNPDTL
FDVQVKRLHEYKRQHLNLLNIVSEYDKLLANPDADVQPKTYIFAAKAAPGYYLAKQIIKL
IWSLGEEIKKNPKISEKLNVVFLENYNVSLSELLMPASEISEQISLAGTEASGTGNMKFM
LNGAVTLGTKDGANVEIGEVVGDDNIINFGMTVEEVERRKKAGYSPVYMYSSDPVIKAAI
DRINSGINGTKFDDVVSSLKYSDPYMVLADFRSYQDAQARASAIYADNLKWQRMSLMNIA
NAGIFSADRAVDEYAKNIWKL

Enzyme Prediction     

EC	2.4.1.1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT35	98	801	3.6e-259	0.9970326409495549

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK14986	PRK14986	0.0	63	799	66	808	glycogen phosphorylase; Provisional
COG0058	GlgP	0.0	7	801	2	748	Glucan phosphorylase [Carbohydrate transport and metabolism].
PRK14985	PRK14985	0.0	36	800	32	795	maltodextrin phosphorylase; Provisional
TIGR02093	P_ylase	0.0	29	801	12	794	glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
cd04300	GT35_Glycogen_Phosphorylase	0.0	15	801	1	795	glycogen phosphorylase and similar proteins. This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCO06148.1	0.0	4	801	5	801
ADU21019.1	0.0	1	801	1	800
CBL18319.1	0.0	14	801	10	798
CBL33913.1	0.0	7	801	3	798
CBK97235.1	0.0	7	801	3	798

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5OX0_A	4.76e-244	33	799	47	826	GlycogenPhosphorylase in complex with CK898 [Oryctolagus cuniculus]
2GJ4_A	7.20e-244	33	799	35	814	Structureof rabbit muscle glycogen phosphorylase in complex with ligand [Oryctolagus cuniculus]
2GM9_A	7.44e-244	33	799	35	814	Structureof rabbit muscle glycogen phosphorylase in complex with thienopyrrole [Oryctolagus cuniculus],5MCB_A Glycogen phosphorylase in complex with chlorogenic acid. [Oryctolagus cuniculus],7ONF_A Chain A, Glycogen phosphorylase, muscle form [Oryctolagus cuniculus]
2FFR_A	7.44e-244	33	799	35	814	Crystallographicstudies on N-azido-beta-D-glucopyranosylamine, an inhibitor of glycogen phosphorylase: comparison with N-acetyl-beta-D-glucopyranosylamine [Oryctolagus cuniculus]
7O8E_A	8.78e-244	33	799	40	819	ChainA, Glycogen phosphorylase, muscle form [Oryctolagus cuniculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9XTL9	2.69e-243	27	799	40	826	Glycogen phosphorylase OS=Drosophila melanogaster OX=7227 GN=GlyP PE=2 SV=2
P00489	7.38e-243	33	799	47	826	Glycogen phosphorylase, muscle form OS=Oryctolagus cuniculus OX=9986 GN=PYGM PE=1 SV=3
P79334	2.02e-242	33	799	47	826	Glycogen phosphorylase, muscle form OS=Bos taurus OX=9913 GN=PYGM PE=1 SV=3
O18751	8.12e-242	33	799	47	826	Glycogen phosphorylase, muscle form OS=Ovis aries OX=9940 GN=PYGM PE=2 SV=3
Q9WUB3	2.30e-241	33	799	47	826	Glycogen phosphorylase, muscle form OS=Mus musculus OX=10090 GN=Pygm PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000070	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001649_01156.