CAZyme Information: MGYG000001650_00216

Basic Information

• Species: F23-B02 sp900760205
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; F23-B02; F23-B02 sp900760205
• CAZyme ID: MGYG000001650_00216
• CAZy Family: GT39
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
582		66182.02	7.7208

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001650	1589868	MAG	United States	North America

• Gene Location: Start: 14708; End: 16456 Strand: -

Full Sequence      

MRLTYVFPVLILLFIVLFYIGCPRLAHADLRKNRRSAFCRRDALALGSIVILYAASAFYN
LGDTEAPQSFHRFDNNESVVIDLGRETQVDGLMFYSGLNTGSYRLEYSPDGLNWTEVGEF
KQSYTALFKWETPELYDGAEAVTRYIRLTAGGDMCLGELAVKSGGKLAEYACSAAELCDE
QDIVPAYQTYLNSTYFDEIYHARTAFEHIEGVYPYEISHPPLGKLIIAAGIKLFGMTPFG
WRFSGTLFGVLMLPVLYIFLKRMFGGTAVPVCGTAMFAFDFMHFTQTRIATIDTYAVFFI
LLMYLFMYLYISGGRLRHLALSGVFFGVGVACKWTCLYAGAGLALIWLIHWIRNFRLREF
IKNCLFCIVFFVLVPAAVYYLSYFPYGQASGLHGAGMFFTKDYADIVLQNQDFMFSYHSG
VHSEHPYSSRWYQWLIDERPILYYLQYYSDGTRSSFGAFLNPVLCWAGLAAIGICAYLAV
FRRDGVSAFIVIGYLAQLLPWVFITRTTFEYHYFPASVFLVLAVCRVFSVMQRSVKHWRF
NVYGLAGLSCALFVLFYPVLSGRTVNAELASALLKWLPSWPF

Enzyme Prediction     

No EC number prediction in MGYG000001650_00216.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT39	191	385	3.3e-53	0.9147982062780269

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam16192	PMT_4TMC	4.68e-20	414	578	10	198	C-terminal four TMM region of protein-O-mannosyltransferase. PMT_4TMC is the C-terminal four membrane-pass region of protein-O-mannosyltransferases and similar enzymes.
COG1928	PMT1	6.14e-16	189	383	43	264	Dolichyl-phosphate-mannose--protein O-mannosyl transferase [Posttranslational modification, protein turnover, chaperones].
pfam13231	PMT_2	2.14e-15	218	375	1	156	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.
pfam02366	PMT	1.74e-13	190	385	17	239	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
COG1928	PMT1	3.34e-13	408	582	502	695	Dolichyl-phosphate-mannose--protein O-mannosyl transferase [Posttranslational modification, protein turnover, chaperones].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANU42918.1	9.10e-165	7	582	15	653
QQR07704.1	9.10e-165	7	582	15	653
QBB67038.1	5.36e-163	7	582	25	752
QIA32159.1	1.03e-162	7	582	11	649
ALP95330.1	1.07e-162	7	582	25	752

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P9WN04	1.75e-20	217	580	100	521	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=pmt PE=3 SV=2
P9WN05	1.75e-20	217	580	100	521	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=pmt PE=1 SV=2
L8F4Z2	1.25e-18	196	580	67	515	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycolicibacterium smegmatis (strain MKD8) OX=1214915 GN=pmt PE=3 SV=1
Q8NRZ6	7.60e-13	217	580	97	519	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) OX=196627 GN=pmt PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000003	0.000002	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
5	22
43	62
238	260
289	311
326	348
361	383
458	480
485	504
508	530
542	561