CAZyme Information: MGYG000001654_00870

Basic Information

• Species: UMGS856 sp900760305
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; UMGS856; UMGS856 sp900760305
• CAZyme ID: MGYG000001654_00870
• CAZy Family: GH18
• CAZyme Description: Spore germination protein YaaH

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
426	MGYG000001654_35|CGC1	46755.06	4.3613

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001654	2698113	MAG	United States	North America

• Gene Location: Start: 4674; End: 5954 Strand: -

Full Sequence      

MEIHVVQPGETLEQIARQYGVSVERIAADNRLNVQSALVPGQTLVILYPAESYTVQPGDT
LDSIAGALGVPVNQLLRNNPILGGYSSIYPGQTLVVSYQQEKEGALSVNGYVYPFVNRDV
LQQALPYLTYLTLFTYGFTTGGDLIELDDEELIGLAKSAGVAPLMHLSTLTPAGNFSSEL
ANLVLNRPDLQEKLIGQVLAVLGAKGYAGLDVDFEFVDPANRQAYIDFIRTMTERLNAEG
YLVVTALAPKTSPNQPGLLYEAHDYEGIGEASNAVLLMTYEWGYSEGPPMAVAPLPSVRR
VLDYGVTAIPPEKIYMGMPNYGYDWPLPFVRGETKAPSIGNVEAVDIARRAGAAIQYDET
SQAPHFNYSQNGQGHEVWFEDARSIRAKLALVPEYGLEGVGYWNLMRPFPQNWLVLNALY
EIRKAL

Enzyme Prediction     

No EC number prediction in MGYG000001654_00870.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	136	405	3e-37	0.8006756756756757

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd02874	GH18_CFLE_spore_hydrolase	2.02e-127	108	416	4	313	Cortical fragment-lytic enzyme (CFLE) is a peptidoglycan hydrolase involved in bacterial endospore germination. CFLE is expressed as an inactive preprotein (called SleB) in the forespore compartment of sporulating cells. SleB translocates across the forespore inner membrane and is deposited as a mature enzyme in the cortex layer of the spore. As part of a sensory mechanism capable of initiating germination, CFLE degrades a spore-specific peptidoglycan constituent called muramic-acid delta-lactam that comprises the outer cortex. CFLE has a C-terminal glycosyl hydrolase family 18 (GH18) catalytic domain as well as two N-terminal LysM peptidoglycan-binding domains. In addition to SleB, this family includes YaaH, YdhD, and YvbX from Bacillus subtilis.
COG3858	YaaH	1.51e-123	1	417	1	419	Spore germination protein YaaH [Cell cycle control, cell division, chromosome partitioning].
cd06549	GH18_trifunctional	3.84e-34	162	405	60	290	GH18 domain of an uncharacterized family of bacterial proteins, which share a common three-domain architecture: an N-terminal glycosyl hydrolase family 18 (GH18) domain, a glycosyl transferase family 2 domain, and a C-terminal polysaccharide deacetylase domain.
smart00636	Glyco_18	1.29e-27	125	405	23	331	Glyco_18 domain.
cd02876	GH18_SI-CLP	3.69e-21	176	405	78	307	Stabilin-1 interacting chitinase-like protein (SI-CLP) is a eukaryotic chitinase-like protein of unknown function that interacts with the endocytic/sorting transmembrane receptor stabilin-1 and is secreted from the lysosome. SI-CLP has a glycosyl hydrolase family 18 (GH18) domain but lacks a chitin-binding domain. The catalytic amino acids of the GH18 domain are not conserved in SI-CLP, similar to the chitolectins YKL-39, YKL-40, and YM1/2. Human SI-CLP is sorted to late endosomes and secretory lysosomes in alternatively activated macrophages.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAB1246914.1	3.71e-185	1	424	1	425
QBB66106.1	5.92e-176	1	425	1	426
ALP93864.1	1.69e-175	1	425	1	426
QQR05785.1	1.26e-170	1	423	1	424
ANU41359.1	1.26e-170	1	423	1	424

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4S3J_A	3.00e-83	1	424	3	429	Crystalstructure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876],4S3J_B Crystal structure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876],4S3J_C Crystal structure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876]
4S3K_A	7.18e-82	2	424	5	431	ChainA, Spore germination protein YaaH [Priestia megaterium QM B1551]
3CZ8_A	6.74e-66	114	405	19	308	ChainA, Putative sporulation-specific glycosylase ydhD [Bacillus subtilis subsp. subtilis str. 168],3CZ8_B Chain B, Putative sporulation-specific glycosylase ydhD [Bacillus subtilis subsp. subtilis str. 168]
4WIW_A	8.36e-27	172	403	78	327	ChainA, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_B Chain B, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_C Chain C, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_D Chain D, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_E Chain E, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2],4WIW_F Chain F, Glycoside hydrolase family 18 [Desulfitobacterium hafniense DCB-2]
4Q6T_A	5.18e-25	135	403	37	322	Thecrystal structure of a class V chitininase from Pseudomonas fluorescens Pf-5 [Pseudomonas protegens Pf-5]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P37531	7.04e-84	1	424	1	427	Cortical fragment-lytic enzyme OS=Bacillus subtilis (strain 168) OX=224308 GN=sleL PE=1 SV=2
P0DPJ9	4.23e-82	1	424	2	428	Cortical fragment-lytic enzyme OS=Bacillus anthracis OX=1392 GN=sleL PE=2 SV=1
Q9K3E4	3.32e-81	1	424	2	428	Cortical fragment-lytic enzyme OS=Bacillus cereus OX=1396 GN=sleL PE=1 SV=1
O05495	4.32e-79	1	425	1	420	Putative sporulation-specific glycosylase YdhD OS=Bacillus subtilis (strain 168) OX=224308 GN=ydhD PE=1 SV=2
O32258	5.80e-26	121	417	45	340	Uncharacterized glycosylase YvbX OS=Bacillus subtilis (strain 168) OX=224308 GN=yvbX PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999992	0.000041	0.000003	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001654_00870.