CAZyme Information: MGYG000001658_00851

Basic Information

• Species: SFFH01 sp900548125
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-74; SFFH01; SFFH01 sp900548125
• CAZyme ID: MGYG000001658_00851
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
962		105613.72	6.8521

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001658	2715075	MAG	United States	North America

• Gene Location: Start: 13017; End: 15905 Strand: -

Full Sequence      

MPKRIGDDRRNFSQKPNAPLTPMESDTPDSGTDSMDKTQLNIPVQRETRRMPEPTHSKSK
RERTSRERTVHPRAHSIFKTRTKKPNFVLSVATTTLRLSFVLALCTALALLGAVVGIAKA
FVDTAPTLDLAALDAQDKTSFIYDSNGNLITDYKGTEDRIMVSIDEIPDMLQNAFIAVED
ARFYEHNGVDVKRIVGALVANFTSGSTQGGSTITQQLIKQTVLSSEQSYKRKLQEAYLAM
ELETRYTKKQILESYLNTIFLGGSYYGVRVAAYGYFGKELDQLTLRECAMLAGLTRSPNY
YNPRSNFYTRNTAGSSTPDITNNRTDYVLRQMRENGLITAQQYTAALDRSTANVLEKSPA
STDMYAYPHYVEYAISDVVDTFLDLNGLENTSANRYAMENKLRTGGYSVYLCLDTEIQEI
VEDTLANWSDYPRLRDPSDKVYQSRNADGTYTEIEQPQAAACVFDYRTGELKAIVGGRYK
PTTRKTLNRASGMTMPVGSSIKPLTVYAPAIDLGASPASIAYNMPVPISGWKDSSGKDSW
PKNYGGGGYKGPQSFRSALRNSYNTAAAQILMTYVGVSRSVEYLHLMGIPDKNINADPFG
LALGSSGITPVQMAVAFGTIANKGVYQQPLSFSRILDSSGNVVVDMHQQQDRHQVFKPST
AYLVVDMLKEAVQSGTGTKAKISSQVVAGKTGTNSDSKGVFFAGMTGWYSASVWIGHDNY
KALSSKATGGNSAAPLWQSFMAKIHKAKNLDSREIIDGAPSDYNLVRVTTCGVSGQLATD
ACYNDVNGYKTITDYWDADSVPTAYCSMHKSVSICTESGLLATDYCPSYSIETRGIVLIP
KGHPLYDYIDTYGDTIRKYLGEFATLQSTSDIANHICQIHDAYTATQEPSELENIVTEAS
NLVYQAYQLVGSSPDLSNDTRRQINTAISAVQTLLSLSPIDYTSLQGAVSNLYSQLQAAG
LM

Enzyme Prediction     

No EC number prediction in MGYG000001658_00851.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	147	333	6.6e-65	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	2.79e-173	158	746	1	530	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	2.82e-161	122	746	39	598	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	1.39e-132	142	748	51	722	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02071	PBP_1b	2.56e-98	157	745	145	683	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
pfam00912	Transgly	7.99e-79	146	333	1	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUC66968.1	0.0	76	883	100	898
QTE72383.1	0.0	76	960	100	971
QTE73372.1	0.0	76	960	100	971
QUA52775.1	0.0	73	883	86	887
QTE69475.1	0.0	76	952	80	952

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7U4H_A	1.65e-73	138	841	19	847	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
4OON_A	2.48e-60	142	748	23	726	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3UDF_A	6.39e-60	142	743	23	714	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
3DWK_A	3.31e-55	142	744	10	588	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]
5FGZ_A	6.11e-51	157	742	154	691	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q0TNZ8	3.35e-119	114	814	45	729	Penicillin-binding protein 1A OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=pbpA PE=3 SV=1
A5I6G4	6.53e-119	116	834	33	727	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=pbpA PE=3 SV=1
A7FY32	6.53e-119	116	834	33	727	Penicillin-binding protein 1A OS=Clostridium botulinum (strain ATCC 19397 / Type A) OX=441770 GN=pbpA PE=3 SV=1
A7GHV1	6.28e-118	116	834	33	727	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) OX=441772 GN=pbpA PE=3 SV=1
Q0SRL7	4.05e-116	120	809	52	724	Penicillin-binding protein 1A OS=Clostridium perfringens (strain SM101 / Type A) OX=289380 GN=pbpA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000050	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
96	118