CAZyme Information: MGYG000001659_01142

Basic Information

• Species: Paraprevotella sp900760455
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Paraprevotella; Paraprevotella sp900760455
• CAZyme ID: MGYG000001659_01142
• CAZy Family: GH89
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1761		193944.81	4.6237

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001659	3410285	MAG	United States	North America

• Gene Location: Start: 41183; End: 46468 Strand: -

Full Sequence      

MRKQILLFCLLLVCAVGSRAAETDAVVKLLERIGGVGAAGRFELVVDEELAENGKDVFVI
APGAGKPCIKGNSVLSLSVGVNWYLNHVAHVNLAWNNLKTDLNSVALPVPTTEEKHVSTA
DYRYYLNYCTFSYSMAFWTWERWEEEIDWMAMRGINMPLALVGADVVWRNVLLELGYTDA
EVNDFVAGPGFQAWWLMNNLEGWGGPNKSWWYERQEQLARKILGRMRELGMTPVLPGYSG
MAPNNVASKLGWQVSDPGSWCYFRRPAFIVPTSENFTKMAELYYKHLEALMGVSPYYSMD
PFHEGGNTSGVDLPAAYTAIQREMDKANPNAKWVIQSWNENPRRECLNTIKAGKLVVLDL
FSDGTPKWGNYGNHEFVYCMLHNFGGRVGMHGRLEKTMKGYYEALRTKPACMKGVGATPE
GIETNPVLYDALFELPWRTECTPGEWLAEHVKARYGTDGCAEAEEAWALLERSAYDCQTG
QQGTTEPVVCARPNLTVNSVSTWSSAHIYYDKEDIIRAAALLLQAKNQLDGVNYRYDLVD
VVRQAVTDRANGLLKQVKAAYDSGDKQDFQYRKDCFLQLILDQDRLLATCPEFRLGRWTQ
MARDVTNEAVGAAGDKDWMEWNARTQITVWGPRNSANSGGLHDYSNREWSGLLKDYHYAR
WKTYFDRLTAGTQQPDWFAVEEAWTKDFTKQYTKETEGDAVQTAQELFERYFSRIGQEEG
TDAHYFAYGEDTDMRKSVTFRAWRGEDFTCPVNLPDGVEATLSIDLNNNGMYENGETFNG
LSCAIPAKATTAAARATLSLTDSTHVIFTLIVADRVTEPRTVTVNTADETMGTVAIAGAE
GNTVTTMDDVTLTATPKAGYDFVSWTNAAGEVVSRNATFTYYGKDNATFTANFLINKWGQ
PEEDLSEWTTVRDYQQYISEMTVTKKGVEPQVIYTASSCPERLFVTVPGVVDMARGGSFR
LDWQDPNGSGLSYCRLSAYMDLDNDGEFTSEGELVALMGNKNTTNPSLSKNGFQILLPLD
MPLGLTHVRLRFDGAWTGGWDAVTDAKPAKASTMRMVYDVLLNVTEHAASTSVIRVQSNN
DKAGTATISGVGDEATVQPGERIILQAQPQNGYVFVKWTDEYDRMVSTEANYSFIPAESG
TFTAHFRAALPELLTIGNWKVRYEEHDGQLTLTEAVEGSGALNVPAAYQMDNEEYPIVAL
GAGFLSGNESVTSLSLPKSVVDLGVKYRHILYQQAWEGDGTNNRLFVPEAPISGSEPWKF
HLEATTDGSAYNSWGSGLVATGSDALASSYNGGFQFYWAKSGNLVVKFAGESDKKEFTHT
AGSASLAVDMDYTPDEGVELTVTNASGQSETYRRSGVRLDDIAQLSSSIPQGMAVTDLTF
VAYGQPDAVLFDGCTKLETIKVSSINFVFRAVNGVLYDRSGQTLLRCPEGMKTRRLVLPA
TLECVGSNAFKGVTGVERLVVSGTLPDTEENAFAQATLVCEVSPQECSAHPSGWTLPLLV
SVAGGETVDAGLLAGASVVEIQAEDPRSGALTADKEGVARWYTRVFGKQAYYPVYFPTDV
KEVVMEYGGRLKSVDVANTFVLYTYDGTGYTKVESTEGGIASGAYMLALKEETSSRPYTF
YLTDKGTAAAGTTLFTGNASLAEETLSDDVYLYNPAANRFERLQWGTLVSPFTAYMRMEG
ENLPDYIDGVEVPPTGIGLLPAESVQVKGRTIVVSGQTSDVTVFTLEGKKVYQGGEHIIT
LPSAGVYLVKVAGRVLKVTLQ

Enzyme Prediction     

No EC number prediction in MGYG000001659_01142.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH89	66	709	7.7e-219	0.9924585218702866

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam05089	NAGLU	1.09e-163	123	439	1	333	Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This central domain has a tim barrel fold.
pfam12972	NAGLU_C	6.56e-89	446	705	1	258	Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This C-terminal domain has an all alpha helical fold.
pfam12971	NAGLU_N	7.74e-16	24	108	1	81	Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This N-terminal domain has an alpha-beta fold.
pfam18998	Flg_new_2	2.43e-08	833	896	2	73	Divergent InlB B-repeat domain. This family of domains are found in bacterial cell surface proteins. They are often found in tandem array. This domain is closely related to pfam09479.
pfam18998	Flg_new_2	0.007	1076	1146	3	70	Divergent InlB B-repeat domain. This family of domains are found in bacterial cell surface proteins. They are often found in tandem array. This domain is closely related to pfam09479.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QFQ12583.1	6.05e-258	2	715	3	721
QCQ31494.1	3.19e-235	2	709	3	715
AUI45172.1	4.48e-235	2	709	3	715
QCQ35767.1	1.74e-234	2	709	3	715
QTO27209.1	3.43e-234	2	709	3	715

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VC9_A	7.02e-141	28	699	176	864	Family89 Glycoside Hydrolase from Clostridium perfringens in complex with 2-acetamido-1,2-dideoxynojirmycin [Clostridium perfringens],2VCA_A Family 89 glycoside hydrolase from Clostridium perfringens in complex with beta-N-acetyl-D-glucosamine [Clostridium perfringens],2VCB_A Family 89 Glycoside Hydrolase from Clostridium perfringens in complex with PUGNAc [Clostridium perfringens],2VCC_A Family 89 Glycoside Hydrolase from Clostridium perfringens [Clostridium perfringens]
7MFK_A	8.67e-141	28	699	184	872	ChainA, Alpha-N-acetylglucosaminidase family protein [Clostridium perfringens ATCC 13124],7MFL_A Chain A, Alpha-N-acetylglucosaminidase family protein [Clostridium perfringens ATCC 13124]
4A4A_A	8.83e-140	28	699	199	887	CpGH89(E483Q, E601Q), from Clostridium perfringens, in complex with its substrate GlcNAc-alpha-1,4-galactose [Clostridium perfringens]
4XWH_A	4.15e-110	18	714	1	714	Crystalstructure of the human N-acetyl-alpha-glucosaminidase [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9FNA3	4.04e-111	42	712	64	805	Alpha-N-acetylglucosaminidase OS=Arabidopsis thaliana OX=3702 GN=NAGLU PE=2 SV=1
P54802	3.43e-110	15	714	21	737	Alpha-N-acetylglucosaminidase OS=Homo sapiens OX=9606 GN=NAGLU PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000262	0.999109	0.000151	0.000160	0.000147	0.000143

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001659_01142.