CAZyme Information: MGYG000001666_01858

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-873
• CAZyme ID: MGYG000001666_01858
• CAZy Family: GH97
• CAZyme Description: Retaining alpha-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
644		73042.59	5.5294

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001666	2832137	MAG	United States	North America

• Gene Location: Start: 72; End: 2006 Strand: -

Full Sequence      

MKHFFLLSFLTLFLSASAKEYRLNSPDGGNSVIVRADGNVCYTFVHHGDTILHKSPVGMT
VGGKKWGSDAVCRSVKRNSNNTELELSVPRRHRILRDNYNSMTFGYGTYDIEFRAYDDAV
AYRFVSKSPEAGAVESETVQFVLNENPDMYVLLTDTIQNSFESTYKQRKLNTFPADSLGL
LPVLAKTGKYNVVLCEAGVYDYPGLFLTGADSCLTGVLAKYPAKEQLVNDDLQYRVEARH
NYLIPSAGRRAFPWRVTACLDSDGDLLGNDIVYLLGNDGDTKEDFSWVKPGKALWDWWND
WDVYEVPFKGGVNTEYYIHLIEFADEHGIEYLMIDAGWSDYKDLLKIADGLDMDRICKYA
ADKGIGIILWTSAANFRRQMKPALEMMEKWGVKGIKIDFFDRNDAQMMTYMEQVASECAA
RKMLVDFHGCCPPDGLRRKYPNIITREGVYGLENSKWRYDVTPEHDLNIAFIRQFSGPMD
FTPGSLNNNHRDRFLATNTGPMSQGTRSHQVAMYVMYDSPMQMVSESTTLLDQNPVSRDF
IMDIPTVWDETVPLESKFGRYAAVARRYGDKWYVAAMNGSDEPVEITVNLDFIPASYTKI
RYHADGVNSDRQAKDFTSRTEYFHPGQLTIKMARGGGYAAIISE

Enzyme Prediction     

No EC number prediction in MGYG000001666_01858.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	11	642	3.7e-166	0.9904912836767037

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam10566	Glyco_hydro_97	4.42e-108	284	545	1	278	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14508	GH97_N	2.99e-41	25	275	1	232	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam14509	GH97_C	8.58e-28	548	643	1	97	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIU93149.1	8.13e-211	18	642	22	645
QUT26078.1	4.64e-210	18	642	22	645
ASM64931.1	5.30e-209	18	642	22	645
QRP59557.1	5.30e-209	18	642	22	645
QQT78558.1	5.30e-209	18	642	22	645

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3A24_A	7.24e-128	25	639	6	637	Crystalstructure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron],3A24_B Crystal structure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron]
5E1Q_A	1.19e-126	25	639	20	651	Mutant(D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482],5E1Q_B Mutant (D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482]
5HQC_A	2.95e-48	23	639	3	655	AGlycoside Hydrolase Family 97 enzyme R171K variant from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQ4_A	4.03e-48	23	639	3	655	AGlycoside Hydrolase Family 97 enzyme from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8],5HQA_A A Glycoside Hydrolase Family 97 enzyme in complex with Acarbose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQB_A	1.03e-47	23	639	3	655	AGlycoside Hydrolase Family 97 enzyme (E480Q) in complex with Panose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8A6L0	4.22e-129	4	639	6	658	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
D7CFN7	7.37e-45	48	590	67	576	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1
G8JZS4	8.29e-32	111	644	142	725	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000299	0.999026	0.000210	0.000152	0.000146	0.000137

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001666_01858.