CAZyme Information: MGYG000001678_01667

Basic Information

• Lineage: Bacteria; Cyanobacteria; Vampirovibrionia; Gastranaerophilales; Gastranaerophilaceae; Zag1
• CAZyme ID: MGYG000001678_01667
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
716		79326.18	9.6239

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001678	1849707	MAG	United States	North America

• Gene Location: Start: 793; End: 2943 Strand: -

Full Sequence      

MAKYYVNKNYKAKKMAKENISKKSRKGGGFFSALKFIFILGCACALAGVASLELYLSSLP
PINNLEQFKPNIVTKIYSSDDEIIKTFTAYTYEKIELKDIPENLKKALIATEDKNFYRHH
GYDIAGLARSTIQNIIAGRVVQGASTITQQLARVLFLNNERTFDRKLKELFIAARIEKTI
SKDQILEMYMNNVYLGSGAYGVEGAAQIYFDKHLKNCNLAELALIAGLPQAPSVYSPFNN
MDLAVQRRNQVLTRMYKMRYITRDEYEKAKEEKVHLAKTPQFYTTNKAPYFCDYVLKELE
KLGFDETEISQGGYKVVTTLDYKAQKAANEAIVKNLKNWGLRGDKNQAAVFAYSPIDGKI
LVYAGGKDYTKSQYDRVTQAVRPPGSSFKPIVYAAAMEKGISPNDMIEDKPITIGQWSPH
NYGNKYRGKIPVYTALMVSSNVCAARLIKEVGIRSVIQTARVLGIETPLEYDYTIALGSN
GVKLFEFTRAYGAFANGGYIVQPYAVERVETSRGKVVYKAPKTKISHQLSMKTSAEMTAM
MKTVITHGTGRAANFGKPAAGKTGTTDDNKDAYFMGYTPNIVAGVWVGNDDNTALNKSIQ
GGTVPALIWKDIMKVATEPYGKAEFNYPEVELMPFAAGSNVKIIGEQEEQNKPVEEEKPA
AAQETAEVPNLPESVKNVDQPKPAQTPAPAPQKPVPAKKETAAPVPIPMAVPQSLH

Enzyme Prediction     

No EC number prediction in MGYG000001678_01667.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	82	256	9.9e-67	0.9830508474576272

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5009	MrcA	0.0	34	629	5	731	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	0.0	93	618	3	530	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	0.0	22	631	7	613	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02071	PBP_1b	2.31e-119	55	617	113	683	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
PRK11636	mrcA	2.18e-111	34	618	4	786	penicillin-binding protein 1a; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AOR38680.1	0.0	1	716	1	716
ACI19842.1	1.04e-151	59	627	49	619
ACK42407.1	3.58e-150	29	627	26	620
AEE14353.1	2.91e-144	30	643	11	619
AWB10056.1	1.41e-141	60	643	36	619

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3UDF_A	1.31e-102	75	618	22	717	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
4OON_A	6.48e-99	62	609	9	715	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
5U2G_A	3.80e-74	57	520	5	566	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
3ZG8_B	5.30e-70	176	590	3	433	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
5FGZ_A	4.00e-65	55	592	122	671	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O66874	2.20e-103	58	628	29	679	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
P70997	1.04e-101	74	629	55	620	Penicillin-binding protein 2D OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpG PE=2 SV=3
Q07806	5.74e-101	34	609	4	742	Penicillin-binding protein 1A OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=mrcA PE=1 SV=2
O87579	1.29e-100	43	616	20	705	Penicillin-binding protein 1A OS=Neisseria lactamica OX=486 GN=mrcA PE=3 SV=1
Q1RKC5	1.54e-100	34	629	12	729	Penicillin-binding protein 1A OS=Rickettsia bellii (strain RML369-C) OX=336407 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999942	0.000075	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
27	49