CAZyme Information: MGYG000001678_01723

Basic Information

• Lineage: Bacteria; Cyanobacteria; Vampirovibrionia; Gastranaerophilales; Gastranaerophilaceae; Zag1
• CAZyme ID: MGYG000001678_01723
• CAZy Family: GT19
• CAZyme Description: Lipid-A-disaccharide synthase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
373		42353.72	9.5852

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001678	1849707	MAG	United States	North America

• Gene Location: Start: 9403; End: 10524 Strand: +

Full Sequence      

MKKLFIVTGEYSGDIHASHVVNALRSINPDIEIEGIGGDNLKNAGVKLFSDQKKMGAVGL
SPKIIIEHLTLGKRLVDYLTRVYKPDAVLLIDYGAFNLSISKFLKRAGIKVFYYIPPQVW
ASRKWRINTIKKNIDEVLCIFPFEKTMYESYGIKTHYCGHPLVSQLSQKAPRDEFFDKHG
FDKSKKLVSIFPGSRVFELKYLMHVFIRTAENLQKKHPDLQFCISHAPNLSDSVYNKYLK
NCPFKVIKGENQALLSVSDALILASGTVALEACLYQTPMIIAYRGPWLFYFIYLMVRCIK
LVALPNIIADKRIVPEILQGDVNVHKISYEIEKLLFDKEYREKNIAELGDVKALLSDKIS
SKEAAKVIADVLS

Enzyme Prediction     

No EC number prediction in MGYG000001678_01723.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT19	5	353	8.4e-106	0.9830508474576272

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK00025	lpxB	1.54e-114	1	372	1	375	lipid-A-disaccharide synthase; Reviewed
COG0763	LpxB	7.53e-99	1	373	1	380	Lipid A disaccharide synthetase [Cell wall/membrane/envelope biogenesis].
pfam02684	LpxB	4.10e-73	4	364	1	368	Lipid-A-disaccharide synthetase. This is a family of lipid-A-disaccharide synthetases, EC:2.4.2.128. These enzymes catalyze the reaction: UDP-2,3-bis(3-hydroxytetradecanoyl) glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate <=> UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6 -beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. These enzymes catalyze the fist disaccharide step in the synthesis of lipid-A-disaccharide.
TIGR00215	lpxB	1.29e-67	6	369	10	384	lipid-A-disaccharide synthase. Lipid-A precursor biosynthesis producing lipid A disaccharide in a condensation reaction. transcribed as part of an operon including lpxA [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]
PRK14089	PRK14089	1.50e-46	1	369	1	345	lipid-A-disaccharide synthase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AOR39331.1	1.40e-230	1	372	1	372
AEG01383.1	2.71e-76	9	372	15	381
ABG58662.1	3.80e-75	3	372	2	367
QPK65076.1	1.95e-74	9	372	15	381
ASQ45794.1	3.37e-74	2	373	5	381

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5W8N_A	7.62e-43	6	335	11	345	LipidA Disaccharide Synthase (LpxB)-6 solubilizing mutations [Escherichia coli BL21(DE3)]
5W8S_A	1.06e-42	6	335	11	345	LipidA Disaccharide Synthase (LpxB)-7 solubilizing mutations [Escherichia coli BL21(DE3)],5W8X_A Lipid A Disaccharide Synthase (LpxB)-7 solubilizing mutations-Bound to UDP [Escherichia coli BL21(DE3)]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B8JE78	4.17e-70	3	372	10	382	Lipid-A-disaccharide synthase OS=Anaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258) OX=455488 GN=lpxB PE=3 SV=1
B4UHR6	1.17e-69	3	372	10	382	Lipid-A-disaccharide synthase OS=Anaeromyxobacter sp. (strain K) OX=447217 GN=lpxB PE=3 SV=1
Q74AT9	3.36e-69	3	372	8	380	Lipid-A-disaccharide synthase OS=Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) OX=243231 GN=lpxB PE=3 SV=1
Q39T49	1.33e-68	2	372	7	380	Lipid-A-disaccharide synthase OS=Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15) OX=269799 GN=lpxB PE=3 SV=1
Q2IL69	5.09e-68	3	372	10	382	Lipid-A-disaccharide synthase OS=Anaeromyxobacter dehalogenans (strain 2CP-C) OX=290397 GN=lpxB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000044	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001678_01723.