CAZyme Information: MGYG000001681_00248

Basic Information

• Species: Ruminococcus sp900761275
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Ruminococcus; Ruminococcus sp900761275
• CAZyme ID: MGYG000001681_00248
• CAZy Family: GT5
• CAZyme Description: Glycogen synthase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
484		56497.23	5.3286

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001681	2557345	MAG	United States	North America

• Gene Location: Start: 13875; End: 15329 Strand: +

Full Sequence      

MKNILFAASECVPFIKTGGLADVVGALPQYLNKDEFDVRVIMPYYTCIPEKYRNDFRYVT
HFYMDYGMRPDGQHVGIMEYEYNGIKFYFVDNEYYFKCDTPYSSDLKWDIERFIFFSKAV
MAIMPVVGFRPDIIHCHDWQASMIPVFLHTSFATNPFYSSTKTIMTIHNLKFQGVWNIDT
FKYNTALPDYMFTPDKLEFHKDANMLKGGLVYADYITTVSETYAEEIKYPFYGEQLDGLL
RARSASLRGIVNGIDYNVFDPANDKQIFAEFNSKNFKEKKAVNKEKLQQQLGLPVDRNKY
MIAIISRLTDQKGLDLVNYAIEQICDEFTQFVVIGTGDPRYENMFRHYQWKYPERVSANI
LYSDDLAHKLYASADAMLMPSLFEPCGLTQLISLRYGTVPIVRETGGLKDTVQPYNEFDG
TGTGFSFANYNADEMLGVINYSKHIYYNHRDQWDKMVQRGMETDFSWNSSARQYEGMYDW
MINW

Enzyme Prediction     

EC	2.4.1.21

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT5	3	478	9e-181	0.9936440677966102

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd03791	GT5_Glycogen_synthase_DULL1-like	0.0	3	478	1	472	Glycogen synthase GlgA and similar proteins. This family is most closely related to the GT5 family of glycosyltransferases. Glycogen synthase (EC:2.4.1.21) catalyzes the formation and elongation of the alpha-1,4-glucose backbone using ADP-glucose, the second and key step of glycogen biosynthesis. This family includes starch synthases of plants, such as DULL1 in Zea mays and glycogen synthases of various organisms.
PRK00654	glgA	0.0	3	483	2	465	glycogen synthase GlgA.
COG0297	GlgA	0.0	3	483	2	480	Glycogen synthase [Carbohydrate transport and metabolism].
TIGR02095	glgA	0.0	3	478	2	470	glycogen/starch synthase, ADP-glucose type. This family consists of glycogen (or starch) synthases that use ADP-glucose (EC 2.4.1.21), rather than UDP-glucose (EC 2.4.1.11) as in animals, as the glucose donor. This enzyme is found in bacteria and plants. Whether the name given is glycogen synthase or starch synthase depends on context, and therefore on substrate. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
PRK14099	PRK14099	6.09e-118	4	478	6	476	glycogen synthase GlgA.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUO21972.1	1.79e-249	1	484	1	483
QEH70303.1	1.41e-248	1	479	1	478
ADZ84828.1	1.34e-246	1	479	1	478
CBK90689.1	1.58e-243	1	482	1	482
CBK94995.1	1.58e-243	1	482	1	482

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3D1J_A	4.00e-86	3	478	2	473	ChainA, Glycogen synthase [Escherichia coli]
2QZS_A	7.02e-86	3	478	2	473	CrystalStructure of Wild-type E.coli GS in complex with ADP and Glucose(wtGSb) [Escherichia coli],2R4T_A Crystal Structure of Wild-type E.coli GS in Complex with ADP and Glucose(wtGSc) [Escherichia coli],2R4U_A Crystal Structure of Wild-type E.coli GS in complex with ADP and Glucose(wtGSd) [Escherichia coli],3GUH_A Crystal Structure of Wild-type E.coli GS in complex with ADP and DGM [Escherichia coli K-12]
3COP_A	5.46e-85	3	478	2	473	ChainA, Glycogen synthase [Escherichia coli],3CX4_A Chain A, Glycogen synthase [Escherichia coli]
1RZU_A	4.24e-84	3	483	2	477	ChainA, Glycogen synthase 1 [Agrobacterium tumefaciens],1RZU_B Chain B, Glycogen synthase 1 [Agrobacterium tumefaciens]
1RZV_A	3.02e-80	3	483	2	477	ChainA, Glycogen synthase 1 [Agrobacterium tumefaciens],1RZV_B Chain B, Glycogen synthase 1 [Agrobacterium tumefaciens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
C4Z0G2	3.21e-233	1	479	1	476	Glycogen synthase OS=Lachnospira eligens (strain ATCC 27750 / DSM 3376 / VPI C15-48 / C15-B4) OX=515620 GN=glgA PE=3 SV=1
A9KMA3	1.05e-212	1	479	1	476	Glycogen synthase OS=Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg) OX=357809 GN=glgA PE=3 SV=1
Q97GX6	1.60e-157	3	478	2	473	Glycogen synthase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=glgA PE=3 SV=1
A0PY73	6.88e-157	3	478	2	472	Glycogen synthase OS=Clostridium novyi (strain NT) OX=386415 GN=glgA PE=3 SV=1
A4J4I4	1.32e-154	3	478	2	471	Glycogen synthase OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=glgA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000052	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001681_00248.