CAZyme Information: MGYG000001681_00816

Basic Information

• Species: Ruminococcus sp900761275
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Ruminococcus; Ruminococcus sp900761275
• CAZyme ID: MGYG000001681_00816
• CAZy Family: PL1
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1260		133567.28	4.8146

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001681	2557345	MAG	United States	North America

• Gene Location: Start: 3684; End: 7466 Strand: +

Full Sequence      

MKNSIMKKRIASIAAAAVVSVSCVAGNLGGVNVINADNSSITAFAAESSVKFIATVGYGE
AMYATWSSVSGASGYNVYVDGVQIDSMLIRQYPGYMRADAVGLKSGSHTMKVVPVINGKE
DASKASETKATAYAHDRSGFGWVNGTSSGAYNEDGTLKSNAVVVYVTDANKDSVKASIDS
TGKGASELTGIQNIILGYKKGKEPRPLCVRFIGNITDPANMPSGDLLVDTVTAGLTIEGI
GNDTVFNGFGLVMKNSSNVEVRNIGFMNCNSKEGDNCGLQQNNDHVWVHNCDFFYGDAGS
DADQVKGDGALDTKTSTYVTHSYNHFWDSGKCNLQGMKSESTENYITYHHNWYDHSDSRH
PRIRTCSVHIYNNYFDGNAKYGVGVTMGASAFVENNYFRNCKYPMLISMQGSDDITGGTF
SDENGGIIKSFGNYITGAKAYTTYKDNPTDFDAYEVANAKDTVPSSVKSKQGGTSYNNFD
TSSVMYNYTPDKAEDVPANVTAKAGRVDGGDLKWTFNNSVDDESYAVNDALKAALVSYKP
TVVAIGSGFKDDNTNPPTSSTTTTTTTTVTTNTPPVVTTVTPPTPTPTPSQPVTGDNVIY
ASPKGGGDGKTMNTPTDVLSAIKSVQAGGTIYLLEGTYSYTSTILIDENNSGSAGKYKTI
SAYPGANVKFDFTGQATAGANRGIVLDGSYWHFYGFEIANAGDNGMLLSGDNNIIEMMVF
NGNQDTGLQLSRYQTSYNTVAQWPSYNLVKNCTSKNNCDDATMENADGFAAKLTCGEGNV
FDGCLSYCNSDDGWDLYAKAETGPIGVVTIKNSIAFRNGFTEDGRGYGDCDGNGFKLGGG
GIGTRHIVENCLAFENLNCGFTDNNNPEFGDMKNCTAYNNSVGGNGKANYMVYRCSSSAT
FDGIVSYTNVSKVSNTGAAGIKLSNDKFVGKMKNSVYYNSKYYQVDSETAMTSGAKLGTV
ITPSVSDFITLEVPAMGTDFHKVWRNADGSPNPGGFAEQPLKSGVNDMGYHMSNGTPQQP
SGSTTTTVSTTTSSSTTTTTTSSQNPTPVSGGYVHNFTENGTASSYYTITGNLSTGKGTV
NYAGKTLTQCLKLESATNITFKAANAGTITLVFAEPAATIKVDGKKYTASGDGIITVDLA
AGDHAVTKADSANLFYMVYSEKQSGQSTTTTTTTTTPNPDIIWGDANCDDIVDIADAVLI
MQSCANPDVYGVNGTDPTHITAQGVINGDVYESGTSGITNQDALSIQKWRLKLIPALPES

Enzyme Prediction     

No EC number prediction in MGYG000001681_00816.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL9	597	999	3e-123	0.968
PL1	230	400	1.6e-45	0.8217821782178217
CBM77	1053	1155	1.1e-41	0.9902912621359223

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3866	PelB	1.19e-51	170	512	41	344	Pectate lyase [Carbohydrate transport and metabolism].
pfam18283	CBM77	4.41e-43	1051	1157	1	108	Carbohydrate binding module 77. This domain is the non-catalytic carbohydrate binding module 77 (CBM77) present in Ruminococcus flavefaciens. CBMs fulfil a critical targeting function in plant cell wall depolymerisation. In CBM77, a cluster of conserved basic residues (Lys1092, Lys1107 and Lys1162) confer calcium-independent recognition of homogalacturonan.
smart00656	Amb_all	5.09e-32	230	402	13	190	Amb_all domain.
pfam00544	Pec_lyase_C	1.75e-21	236	398	38	211	Pectate lyase. This enzyme forms a right handed beta helix structure. Pectate lyase is an enzyme involved in the maceration and soft rotting of plant tissue.
cd14256	Dockerin_I	0.007	1184	1248	2	54	Type I dockerin repeat domain. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. The cellulosome consists of scaffoldin, a noncatalytic scaffolding polypeptide, that comprises repeating cohesion modules and a single carbohydrate-binding module (CBM). Specific calcium-dependent interactions between cohesins and dockerins appear to be essential for cellulosome assembly. This subfamily represents type I dockerins, which are responsible for anchoring a variety of enzymatic domains to the complex.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AOR96287.1	1.26e-262	38	997	42	1073
QMW93302.1	3.09e-260	38	997	42	1073
BBK78741.1	3.09e-260	38	997	42	1073
ABX41986.1	1.60e-259	40	998	29	1144
ADU23076.1	6.05e-254	1	554	1	556

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5FU5_A	2.30e-42	1048	1160	3	114	Thecomplexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition [Ruminococcus flavefaciens]
1RU4_A	1.24e-34	598	929	16	344	ChainA, Pectate lyase [Dickeya chrysanthemi]
5M2S_B	1.47e-24	1182	1259	27	103	R.flavefaciens' third ScaB cohesin in complex with a group 1 dockerin [Ruminococcus flavefaciens FD-1]
1AIR_A	3.62e-23	167	505	2	339	ChainA, PECTATE LYASE C [Dickeya chrysanthemi],1O88_A Chain A, Pectate Lyase C [Dickeya chrysanthemi],1O8D_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8E_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8F_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8G_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8H_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8I_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8J_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8K_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8L_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1O8M_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi],1PLU_A Chain A, Protein (pectate Lyase C) [Dickeya chrysanthemi],2PEC_A Chain A, PECTATE LYASE C [Dickeya chrysanthemi]
2EWE_A	8.85e-23	167	505	2	339	ChainA, Pectate lyase C [Dickeya chrysanthemi]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0C1A6	1.79e-34	598	929	41	369	Pectate lyase L OS=Dickeya chrysanthemi OX=556 GN=pelL PE=3 SV=1
P0C1A7	1.08e-33	598	929	41	369	Pectate lyase L OS=Dickeya dadantii (strain 3937) OX=198628 GN=pelL PE=1 SV=1
P22751	2.70e-33	599	880	391	639	Pectate disaccharide-lyase OS=Dickeya chrysanthemi OX=556 GN=pelX PE=1 SV=1
B1B6T1	1.63e-22	182	463	60	330	Pectate trisaccharide-lyase OS=Bacillus sp. OX=1409 GN=pel PE=1 SV=1
Q8GCB2	1.63e-22	182	463	60	330	Pectate trisaccharide-lyase OS=Bacillus licheniformis OX=1402 GN=pelA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000799	0.221159	0.777612	0.000156	0.000148	0.000104

TMHMM  Annotations     

start	end
12	34