CAZyme Information: MGYG000001682_02327

Basic Information

• Species: Paramuribaculum sp900760855
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; Paramuribaculum; Paramuribaculum sp900760855
• CAZyme ID: MGYG000001682_02327
• CAZy Family: GH85
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1290		141728.32	4.8827

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001682	2633261	MAG	United States	North America

• Gene Location: Start: 334; End: 4206 Strand: -

Full Sequence      

MKKTLFVASGLAFVAMAGNAQQLKEGYIQWPNSYALHRYVEAWTPGQDLKQTYSGESLSP
EAWEDEEFFTSRVKLRPLITNTATQIYPELTGVKDKKLLFWVPCGLALEGQYHQGCLPNA
KFDSEVFSAWSYVTHFGEWTSPYGWVPGGFADAAHKHGVLVSGVASIPNAALTGNWLDCM
SAMGSSFATDEGAEKLAKFLMYHGSDGMGYNSEFSTNSTIMSGIRTMHEKVVKNMKAKGH
VGPENPWYGGTGDNGSIGFDQGLRNQQENFGNAGTPRTTLFLNYNWSTSTYFSTKLDSYI
ASSGRDSRDVYLGQNMQGGMSKDGGNGREWETHMNEGANYSIGLWGAHQNNLLFPGRASY
GGTSMLMQKGYQNILEQWFTNAKRNPAAKMPLSTSTSLNVHPEFQGMSRMMSARSAYGWD
LGEEPFITYFGLGNGQDYKIAGEVANATEWYNLGMQDYTPTWRWWWADEILGNEPSNIPA
NCMEAAYTWEDAWTGGSSLQITGSTTAPQYLHLFKTAFNVKTNDKVVVRYKLLNGSADLN
FFYTKIGNEGTELTPAQTKIMTQAGDKDGKKIFKDEWYTVTMDVKTRGGLSAGDWALMGL
KVANAKDLDVLIGEISIIRGTAATPSMPKIKLAKALANTVYGVDAKLIWSMDHSKAAGEP
VYNTDVNTSHYRMWAQIENEDPVFVGATTSWAGILYRIPNPKHAGRVKLGVQAVSLDHKS
ASDIAWTTDWLTIPAHEVSEEVAIDKTCIKPGEEFRIGFVDPMHPNAVLSLYDHKGTKVA
TNNGNSIELVASLDEIGAYDLVANEGTDKESRYNCFAQVSSWEVGALPEITSLTVNGEES
SDNVTFKMNEPIEFGYTGRQADGKGSAGIRTNYKILGGPMSEFGIGQYKSFSVSFWYRPT
LYTDQQQNLMGVEDRTGGWPKNNWGWFWSELNSTGGVSAYTMRGSAANPNEYQYEYPAST
ALPLGAWTHVTMVFDYDSSNAMLMKLYLNGVYQVPSGWGLGNKDNSVDANGLPGRGPNRD
VFDGGMWMHIMGGRGEKPGFSDGVIDDMTIWNGSITEEDIAKAMVGLDADNLPDNVQCFW
DFDSNYDEASKAFVSKGKNTTAHFGSFDLIQGEKEGQSVQTFIEPELQAGCPYVKGDGFT
VTTVPTWSARQSAIEIVDGNDKEGKAKIAFGANGDKTVKLTLKNSWGEDSAEYPVVHISN
EEYNAIDGIDADVTGGIKTYSINKTIFVEFADGGNYEVSVYNVSGMLEGRDARYVNAGEV
MNIGIANSGVYLISVAKDGRQLRTIKVINK

Enzyme Prediction     

No EC number prediction in MGYG000001682_02327.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH85	119	438	2.4e-38	0.9523809523809523

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4724	COG4724	3.97e-30	59	617	43	549	Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism].
cd06547	GH85_ENGase	2.29e-17	108	465	11	334	Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model.
pfam13385	Laminin_G_3	1.86e-12	889	1061	17	151	Concanavalin A-like lectin/glucanases superfamily. This domain belongs to the Concanavalin A-like lectin/glucanases superfamily.
pfam03644	Glyco_hydro_85	4.33e-11	121	435	4	291	Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates.
smart00560	LamGL	1.62e-05	890	991	2	84	LamG-like jellyroll fold domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT74450.1	0.0	1	1290	1	1253
AHF11930.1	0.0	1	1290	1	1253
QFQ13703.1	1.69e-298	10	1290	10	1247
EFC71164.1	7.38e-261	20	1290	22	1247
BBA29896.1	1.10e-255	1	1290	1	1244

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3FHA_A	1.12e-19	60	549	21	468	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_C Chain C, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
2VTF_A	1.13e-19	48	549	3	473	X-raycrystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae],2VTF_B X-ray crystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae]
3FHQ_A	1.47e-19	60	549	21	468	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_F Chain F, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000542	0.998590	0.000363	0.000178	0.000167	0.000149

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001682_02327.