CAZyme Information: MGYG000001685_00775

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; CAG-964
• CAZyme ID: MGYG000001685_00775
• CAZy Family: GH13
• CAZyme Description: Glycogen debranching enzyme

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1108		120259.41	5.1129

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001685	2227904	MAG	United Kingdom	Europe

• Gene Location: Start: 45065; End: 48391 Strand: +

Full Sequence      

MRMRKGISVALVMTMVGTLVVGNASAATLNQVKASAYYNSSDNYATYSGNDLGANYSKTS
TTFKVWAPTASAVSLNLYKTGSDGESGAGKISTTSMTKNSSNNVWSVTVSGDLNGVYYTY
SVTNNGQTKETQDVYSTATGVNGNRSMVIDLDSTDPDNWENDQHMLVDNQTDAVIWEVHV
RDFSISNSSGVSTQNKGKYLAFTEDGTKINGTDSLSTCVDYLVDQGVNYVHLNPIYDYGS
VDETKLNTAQYNWGYDPVNYNVPDGSYSTNPYDGKVRINEFKQMVQALHSRGIGVVMDVV
YNHMYSATNSSFEKTVPGYYFRMKSSSAFYDGSGCGNVTASDKAMFRKFMVDSVMYWANE
YHIDGFRFDLMGCHDITTMNTIREKLDTIDKRILIYGEPWMADWNSNGISSGEACIMDNA
SKVNSRVAMFSDKIRNALKGGTDDATQGYIQGSSTVNNDIKAGMMGGCSTTFGKWAQAPS
QCITYNSAHDNLSLWDKILKSNGSSDYNSTNNTFLAQNKLSAAIVLTSQGIPFYMAGEEF
ARTKQGDHNSYKSPDSINAIDWTRVKTYNNLVQYYKGLMAIRRVYSPFRAADNSAINTTY
CVETGSAIGYTIRNTSSNASKEWGTVAVLVNNASYAQTLSLKMNGGTMPSNWVIVANGDK
AGLEQLGTVSGSSISVPARSAMVLVDASTFNNLVIDQPVYQTVTTKHIEKSTGNVLKTSS
VQVKQGNTYKTSADSALLEDYLLVGTEGVESGTVGTQDITVIYYYQKDTTPSYTLTTKYV
SQTGTELKASETKKLKQGATYSAVPQTISGYELDTTKLPATASGTISANTEVVFVYKSTD
ATKGVKVHYYNANNWGSVTIYAYDERTGTTKMLSGGWPGSAMMAEGNGWYTYPEINSDYA
QVIFANGSGTQEPGANQPGYEVSGEVWLKNGVSYFGSKIVVSHIDTNGKKLADDVVIEGT
QKSTSDTYTTSAISSLGTVVTTLGNTTGNWSKDTINVIYVYESRTEPHTDPTEPPTEPTE
PPTEPDYQTVTIGEVSGDGRISIADAILVMRKTLDGNVSQTDILTKTKMDANKDGKISGA
DAVLILRYAMGSRDTGYVGQKVQVLITG

Enzyme Prediction     

No EC number prediction in MGYG000001685_00775.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	220	539	1.6e-109	0.9930795847750865
CBM26	846	920	1.2e-17	0.9333333333333333

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02104	pulA_typeI	0.0	41	664	1	604	pullulanase, type I. Pullulan is an unusual, industrially important polysaccharide in which short alpha-1,4 chains (maltotriose) are connected in alpha-1,6 linkages. Enzymes that cleave alpha-1,6 linkages in pullulan and release maltotriose are called pullulanases although pullulan itself may not be the natural substrate. This family consists of pullulanases related to the subfamilies described in TIGR02102 and TIGR02103 but having a different domain architecture with shorter sequences. Members are called type I pullulanases.
cd11341	AmyAc_Pullulanase_LD-like	0.0	171	582	1	406	Alpha amylase catalytic domain found in Pullulanase (also called dextrinase; alpha-dextrin endo-1,6-alpha glucosidase), limit dextrinase, and related proteins. Pullulanase is an enzyme with action similar to that of isoamylase; it cleaves 1,6-alpha-glucosidic linkages in pullulan, amylopectin, and glycogen, and in alpha-and beta-amylase limit-dextrins of amylopectin and glycogen. Pullulanases are very similar to limit dextrinases, although they differ in their action on glycogen and the rate of hydrolysis of limit dextrins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.
COG1523	PulA	1.24e-128	62	688	70	693	Pullulanase/glycogen debranching enzyme [Carbohydrate transport and metabolism].
TIGR02103	pullul_strch	2.12e-116	49	685	125	895	alpha-1,6-glucosidases, pullulanase-type. Members of this protein family include secreted (or membrane-anchored) pullulanases of Gram-negative bacteria and pullulanase-type starch debranching enzymes of plants. Both enzymes hydrolyze alpha-1,6 glycosidic linkages. Pullulan is an unusual, industrially important polysaccharide in which short alpha-1,4 chains (maltotriose) are connected in alpha-1,6 linkages. Enzymes that cleave alpha-1,6 linkages in pullulan and release maltotriose are called pullulanases although pullulan itself may not be the natural substrate. This family is closely homologous to, but architecturally different from, the Gram-positive pullulanases of Gram-positive bacteria (TIGR02102). [Energy metabolism, Biosynthesis and degradation of polysaccharides]
PLN02877	PLN02877	1.74e-109	22	563	191	798	alpha-amylase/limit dextrinase

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QCT07019.1	2.47e-278	23	691	42	711
QCT05892.1	4.32e-212	3	908	1	1004
QCT05893.1	8.36e-210	1	858	1	849
APO32485.1	9.01e-210	15	692	3	674
QCT07748.1	7.12e-195	15	892	2	973

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7LSA_A	9.02e-222	47	771	59	797	ChainA, Pullulanase [Ruminococcus bromii]
7LSR_A	1.42e-220	47	771	59	797	ChainA, Pullulanase [Ruminococcus bromii]
7LSU_A	1.52e-220	47	771	59	797	ChainA, Pullulanase [Ruminococcus bromii]
7LST_A	1.57e-220	47	771	59	797	ChainA, Pullulanase [Ruminococcus bromii]
2WAN_A	1.45e-146	47	684	313	919	Pullulanasefrom Bacillus acidopullulyticus [Bacillus acidopullulyticus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O33840	2.63e-119	47	684	220	840	Pullulanase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=pulA PE=1 SV=2
C0SPA0	1.75e-103	47	592	101	626	Pullulanase OS=Bacillus subtilis (strain 168) OX=224308 GN=amyX PE=1 SV=1
Q8GTR4	1.86e-65	42	563	199	791	Pullulanase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PU1 PE=1 SV=2
P07206	2.69e-53	53	563	305	911	Pullulanase OS=Klebsiella pneumoniae OX=573 GN=pulA PE=1 SV=2
P07811	3.65e-53	53	563	315	921	Pullulanase OS=Klebsiella aerogenes OX=548 GN=pulA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000270	0.998983	0.000202	0.000185	0.000180	0.000159

TMHMM  Annotations     

start	end
7	29