CAZyme Information: MGYG000001685_01474

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Acutalibacteraceae; CAG-964
• CAZyme ID: MGYG000001685_01474
• CAZy Family: GH77
• CAZyme Description: 4-alpha-glucanotransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
508	MGYG000001685_28|CGC1	59077.5	6.4271

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001685	2227904	MAG	United Kingdom	Europe

• Gene Location: Start: 20805; End: 22331 Strand: -

Full Sequence      

METKLKRGSGVLLHITSLPGLFGIGDLGKEAYKFVNFLKRAKQMYWQILPTGEIGEENSP
YKCCSAFAGNWLMISIEQLMDAGLLSFDDLPFETDYLHKEVNYEAVKVLKYKLLMTAYKK
FKELPQNTLLNKEYQTFIKENQYWLDDYAAFKVAAKRFGTQNWSAWDSDIKKRNPDALKH
LCAEYFDIYESSKFIQFLFFKQWKALKQYANNNGIKIIGDIPIYVDYESADVWSSPKLFD
LDEDLRCKNVAGVPPDYFAKNGQIWGNPLYNWKAHKDTNFDWWNKRVQFTLKMVDYLRID
HFKGFSSYYAIPARNASAANGEYFSAPGMDLLKSFQKNIQDMPIIVEDLGNVDKDLEDLR
DSFSLPGMRILQLGFKNKNTSGYHPEFLPHNYLPNSIVYTGTHDTMTVKQWFEDESIDSS
EIENFENYIHKKCTKEDICWEMIRLLLSSVASVAIIPLQDILELGEEGRMNYPGTLGKSW
KWRYLEKDLNLRSAKKLAELTELYGRAQ

Enzyme Prediction     

No EC number prediction in MGYG000001685_01474.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH77	13	500	1.6e-158	0.9858299595141701

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK14508	PRK14508	0.0	5	507	3	497	4-alpha-glucanotransferase; Provisional
pfam02446	Glyco_hydro_77	0.0	13	484	1	457	4-alpha-glucanotransferase. These enzymes EC:2.4.1.25 transfer a segment of a (1,4)-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or (1,4)-alpha-D-glucan.
PLN02635	PLN02635	5.76e-159	6	506	27	525	disproportionating enzyme
COG1640	MalQ	4.67e-157	4	507	11	515	4-alpha-glucanotransferase [Carbohydrate transport and metabolism].
TIGR00217	malQ	2.21e-135	5	506	12	513	4-alpha-glucanotransferase. This enzyme is known as amylomaltase and disproportionating enzyme. [Energy metabolism, Biosynthesis and degradation of polysaccharides]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADL12364.1	9.53e-169	4	506	2	495
AGB40998.1	2.45e-151	4	501	2	497
BAY09072.1	1.68e-150	7	507	5	498
ABK61761.1	1.71e-150	6	506	3	492
AVM68969.1	1.76e-150	4	508	11	507

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1TZ7_A	1.86e-135	7	506	22	503	Aquifexaeolicus amylomaltase [Aquifex aeolicus],1TZ7_B Aquifex aeolicus amylomaltase [Aquifex aeolicus]
6M6T_A	2.42e-122	6	506	4	496	ChainA, 4-alpha-glucanotransferase [Streptococcus agalactiae],6M6T_B Chain B, 4-alpha-glucanotransferase [Streptococcus agalactiae],6M6T_C Chain C, 4-alpha-glucanotransferase [Streptococcus agalactiae],6M6T_D Chain D, 4-alpha-glucanotransferase [Streptococcus agalactiae],6M6T_E Chain E, 4-alpha-glucanotransferase [Streptococcus agalactiae],6M6T_F Chain F, 4-alpha-glucanotransferase [Streptococcus agalactiae],6M6T_G Chain G, 4-alpha-glucanotransferase [Streptococcus agalactiae],6M6T_H Chain H, 4-alpha-glucanotransferase [Streptococcus agalactiae]
1CWY_A	1.96e-119	4	502	2	495	CrystalStructure Of Amylomaltase From Thermus Aquaticus, A Glycosyltransferase Catalysing The Production Of Large Cyclic Glucans [Thermus aquaticus],1ESW_A X-Ray Structure Of Acarbose Bound To Amylomaltase From Thermus Aquaticus. Implications For The Synthesis Of Large Cyclic Glucans [Thermus aquaticus]
1FP8_A	2.77e-119	4	502	2	495	StructureOf The Amylomaltase From Thermus Thermophilus Hb8 In Space Group P21212 [Thermus thermophilus],1FP9_A Structure Of Amylomaltase From Thermus Thermophilus Hb8 In Space Group C2 [Thermus thermophilus]
2X1I_A	2.77e-119	10	506	8	499	glycosidehydrolase family 77 4-alpha-glucanotransferase from thermus brockianus [Thermus brockianus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O66937	2.68e-135	7	506	2	483	4-alpha-glucanotransferase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=malQ PE=1 SV=1
P72785	2.04e-134	6	506	4	499	4-alpha-glucanotransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=malQ PE=3 SV=1
Q59266	1.37e-127	13	508	1	487	4-alpha-glucanotransferase OS=Clostridium butyricum OX=1492 GN=malQ PE=1 SV=1
P0A3Q1	4.83e-127	6	506	3	495	4-alpha-glucanotransferase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=malQ PE=3 SV=1
P0A3Q0	4.83e-127	6	506	3	495	4-alpha-glucanotransferase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=malQ PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000067	0.000003	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001685_01474.