CAZyme Information: MGYG000001689_00195

Basic Information

• Species: Blautia coccoides
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia; Blautia coccoides
• CAZyme ID: MGYG000001689_00195
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase BoGH2A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
828	MGYG000001689_1|CGC6	93703.41	5.816

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001689	6086770	Isolate	United States	North America

• Gene Location: Start: 211985; End: 214471 Strand: +

Full Sequence      

MNTTHYPIDKNCRFHLSDCPDAWQAWYDDSDWESVSLPHDWSVTLPFSREYSSGTGYLAG
GIGWYRFRITPHESWKGKRLYLAFDGVYKNSRVWCNSYYLGNRPNGYISFTYDITEQFSF
DRENIICVCADHRDIADSRWFTGSGITRKVQLIVQEPVHMVPWSMAFETPEVSSQKAAFC
ASCEIKNDTLEEVQVTAKFRLIKTENTKSNIENCNFPAHETPPENSHTSSYTENRIQAEA
SVNMRIPAGAACRAKVAGEVLSPVLWSPDSPDLYTLETWICPADSVSGQACHAEAYRVDS
RKVGIRSIRFDADTGFYLNDKNMLIKGVCVHHDAGCLGAAVPVPVWERRLKKLKDMGCNA
IRMSHNPHMPELYDLCDSMGFLVIDEAFDEWEGPKNKWSTGHNVYPPKHQGYYLDFPRWH
REDLTDLVRRDRCHPSIIMWSIGNEIDYPNDPYCHASFTTMTGNNDADKPAEERQYNPKR
PDAGRLAVLAERLTKIVKESDKTRPVTLAAAFPELSSRLGFLDSLDVAGYNYKEHLYDES
HRRFPDKPFIGSENGHSLEAWEAVTKNPYISGQFLWTGIDYLGEAKGWPIHGSDAGLLTL
AGFEKPGYYRRQSLWSAKPMVHLSTARLDTDQGEWTPVSDTWNYPEKEKVLVKCYTNLPQ
VELWLNDKCLGTYQKPPACDAVMINTDYVPGTLKALGKASDGTEVSHTLTTTGTACRISL
RCYEDILPSAAKPTFRQIEVLMKDTWGNPVLSDCSLLHVEAENGQILGLENGDLTDVTDY
SAAFRRAYHGRLLIYVNHIDEEKEMTVTVFGDAEANIKTAVLTLSSLK

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	8	615	1.4e-108	0.6037234042553191

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.50e-60	20	651	48	635	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	1.59e-30	20	448	60	452	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02836	Glyco_hydro_2_C	8.45e-27	313	582	5	246	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10150	PRK10150	4.47e-26	64	508	68	447	beta-D-glucuronidase; Provisional
pfam02837	Glyco_hydro_2_N	1.97e-24	13	153	9	166	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIB55323.1	0.0	1	828	1	828
QMW76814.1	0.0	1	828	1	828
QBE95340.1	0.0	1	828	1	828
QJU15852.1	0.0	1	828	1	821
ASU31149.1	0.0	1	828	1	821

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5T9A_A	9.68e-154	8	801	32	823	Crystalstructure of BuGH2Cwt [Bacteroides uniformis],5T9A_B Crystal structure of BuGH2Cwt [Bacteroides uniformis],5T9A_C Crystal structure of BuGH2Cwt [Bacteroides uniformis],5T9A_D Crystal structure of BuGH2Cwt [Bacteroides uniformis],5T9G_A Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis],5T9G_B Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis],5T9G_C Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis],5T9G_D Crystal structure of BuGH2Cwt in complex with Galactoisofagomine [Bacteroides uniformis]
3GM8_A	5.77e-116	10	811	11	786	ChainA, Glycoside hydrolase family 2, candidate beta-glycosidase [Phocaeicola vulgatus ATCC 8482]
6B6L_A	1.52e-110	8	808	10	762	Thecrystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838],6B6L_B The crystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838]
7RSK_A	6.24e-110	8	808	10	762	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7RSK_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
5T98_A	2.50e-108	8	825	30	819	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	1.79e-129	8	826	47	848	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	3.68e-90	8	812	51	812	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	5.28e-41	28	709	75	716	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KN75	3.54e-34	4	720	27	692	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
P77989	3.71e-30	18	442	18	384	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999935	0.000107	0.000021	0.000000	0.000000	0.000001

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001689_00195.