CAZyme Information: MGYG000001689_03357

Basic Information

• Species: Blautia coccoides
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia; Blautia coccoides
• CAZyme ID: MGYG000001689_03357
• CAZy Family: GH30
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1347	MGYG000001689_12|CGC3	149198.82	4.0499

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001689	6086770	Isolate	United States	North America

• Gene Location: Start: 116577; End: 120620 Strand: -

Full Sequence      

MRKKDRHKKIKEEIWAFVIGTSMIFSAAAPVMADVNRVQEVNITVNPDIHHQTLEGWGTS
LCWWGNVIGSWGDADWNEDGRPDREEIAEFAFSPEYLNLNIVRYNVGGGDRENSSIKRCE
GLVPGWTEDMTGTADGSASFDPESFYGKNTEEMSDAGQLWMLEQANQYRKAYAEKISKEN
DIINKVFSNSPPYYMTKSGTSTGGSWGDPNNLKDDCYDDFAIYMARATKWVDENLESKFG
TGVSYVEPLNEPDTNYWMEGSTKQEGCIFNNGEVQSKAYREMQSAMDAEEFQGTLNDVKI
TGTDETSLWNAINSFNRLDDEVKAGMETISAHTYSGNNSERKELYRIAESNDKGLWMSEV
TKGGGSHYEGCHESMDAANTRSQSEGIMADLKYMKPSAWIAWLVADSEYECLQTNSNWGL
LHAVFEKDGQPVPGYHTNLLDSSGNRKEGVPGEGYWAVTKQFYTMMQYSKYLKAGYIMID
IDDSNMCAAISPEGDELVIVAQNFEGTREDTIDLSVFNGADTAELYRTTDTESCQLTETQ
DVTDGVLDVILPGNSVSTYVIKASDGAAVCDPDNYSKIVEADVVDPEKSWASESNKFSYE
GSWGESAEEYGGGKYSSAEDASVTFTFDGTQAVIYGSKAPEGAAVLVSVDGKEPEDVFLD
AENKDGEALLYYTGQLEPGRHTIAITMAAGQEGRLLEINYAKIIYGTLEEKIEKIQDIGP
LYTAEGVTPELPNMVYAQTNIGNIIEKEVTWDLEGVDFTEDVVLKGYIEGTSAAASIKVQ
VVPENIRYFIDCNNPQSPEYSKTDSYAELLNETPDRKYSEGSWGYLDEYGVYDGNKEDNY
DVGWYAHPGQEIRYTVPLDTGTYKMTLGFKEWWDQDRPMKVSIVTDEGTTELGKANTWNN
GNPWNEEAYEFTISKEGNAIITVSADGGPEPVLSYIRIQGLLDTQGLKNTIFQAEAIDRS
QYGEEDLAALDGLIRNAKSLFYRASTTQEVLDQTAGELKAKLEEMAGQTQTEADMAGLHM
AVLMAEKMEVQQAESQCYTEESWAAAAEALETARAVIENPNALQEEVDTAFLNLITACNM
LEAGVQKVGLKAAIQGAEAVLMDQETIKQYTEESVQAVRDALAFAQSVFEKDVDQETVNQ
ASRALMDAVTGMLVKEENARLDILIRKAEEFLEKEDQYTPSSVERLRSAIEYAKTAENAE
DAWGQLAEAMTSLVRRANKTELKNTIDKAEEILSQSEKYVESSIAELQILTDEAKLVYDD
NEADHVRVGEALKKLIDEILKARLMGDVNLNGAVDTEDSAVLLQYNAELTELTDEQLEAA
DTDKSGAADSGDAAVILQFAAEKIVSF

Enzyme Prediction     

No EC number prediction in MGYG000001689_03357.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH30	43	559	3.4e-122	0.9978070175438597

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam14587	Glyco_hydr_30_2	9.04e-20	40	403	1	354	O-Glycosyl hydrolase family 30.
cd14256	Dockerin_I	4.42e-09	1286	1339	2	55	Type I dockerin repeat domain. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. The cellulosome consists of scaffoldin, a noncatalytic scaffolding polypeptide, that comprises repeating cohesion modules and a single carbohydrate-binding module (CBM). Specific calcium-dependent interactions between cohesins and dockerins appear to be essential for cellulosome assembly. This subfamily represents type I dockerins, which are responsible for anchoring a variety of enzymatic domains to the complex.
COG5520	XynC	1.36e-08	171	560	100	427	O-Glycosyl hydrolase [Cell wall/membrane/envelope biogenesis].
pfam00404	Dockerin_1	1.37e-04	1286	1336	1	51	Dockerin type I repeat. The dockerin repeat is the binding partner of the cohesin domain pfam00963. The cohesin-dockerin interaction is the crucial interaction for complex formation in the cellulosome. The dockerin repeats, each bearing homology to the EF-hand calcium-binding loop bind calcium.
cd14255	Dockerin_III	1.53e-04	1286	1342	1	62	Type III dockerin repeat domain. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. Two specific calcium-dependent interactions between cohesin and dockerin appear to be essential for cellulosome assembly, type I and type II. This subfamily represents the atypical type III dockerins and related domains.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIB57138.1	0.0	1	1347	1	1347
QMW80085.1	0.0	1	1347	1	1347
AWY99102.1	0.0	1	1320	1	1332
ADL05173.1	1.10e-282	43	1016	44	1269
QJU15030.1	3.20e-123	116	1016	302	1055

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3CLW_A	8.32e-13	51	566	16	501	Crystalstructure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3CLW_B Crystal structure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3CLW_C Crystal structure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3CLW_D Crystal structure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3CLW_E Crystal structure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343],3CLW_F Crystal structure of conserved exported protein from Bacteroides fragilis [Bacteroides fragilis NCTC 9343]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A0A401ETL2	4.05e-32	10	571	4	617	Exo-beta-1,6-galactobiohydrolase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=bl1,6Gal PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001043	0.997836	0.000268	0.000292	0.000264	0.000237

TMHMM  Annotations     

start	end
13	35