CAZyme Information: MGYG000001697_01078

Basic Information

• Species: Erysipelatoclostridium saccharogumia
• Lineage: Bacteria; Firmicutes; Bacilli; Erysipelotrichales; Erysipelatoclostridiaceae; Erysipelatoclostridium; Erysipelatoclostridium saccharogumia
• CAZyme ID: MGYG000001697_01078
• CAZy Family: GH31
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2155	MGYG000001697_15|CGC2	235628.43	4.0521

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001697	3141483	Isolate	Germany	Europe

• Gene Location: Start: 35465; End: 41932 Strand: -

Full Sequence      

MKKKTIHKIGALSLAAVLSLSTVSTSMMGLAAKNALSNEITQATDDAGYNVLGAVTSAVL
DGNKVDLTIQTGEKIRFTFLEQNVFRMYMAPEGEPFREYPVANSSDHTATITNKTDEQYE
SEYNVVPTLEETDTTVTISTEKIKIEINKAQTLIKLMKADGTVVWEEAAPLKYKTGSTIQ
TLKTDEDEYFFGGGTQNGRFSHKGKTIKIATTNTWVDKSVTSPNPFYWSTDGYGVVRNTW
KPGEYDFGSNDGNVVTTTHNEKRFDAYYFVDDEPVDILGDYYELTGNPAELPEYASYLGH
LNCYNRDYWLEVPEGTSGAVKLGDKWYKESQSDNGGEKETLLGDANTTAQQIIEDHEEND
MPLGWFLPNDGYGCGYGQTDSQAGDIENLGNFADYAISKGVQTGLWTQSNLWPADSSNPQ
KGERDIYKEVEAGVHSVKTDVAWVGAGYSMALNGISVAYDAIASKSGLKPNIVTLNGWAG
TQRYGGIWSGDQTGGQWEYIRFHIPTYIGTALSGQPNIGSDMDGIFGGRNSTVQTRDFQW
KAFTTYMLDMDGWGSNQKSPWALGEDATSINRTYLKLKAQLMPYINTISHEATAEGGLPM
IRAMFLEEANAYTLGTSTQYQYMWGDNFIVAPIYQNTNADAQGNDIRNDIYLPSTSDIWI
DYFTGQQYRGGQVLNNFDAPIWKLPVFVRNGSIIPMYPENNNPEAVSETNTSGLDRSQRI
VEFYPYGSSEFEAYEDDGKTLGGASVTTLLTSDVENGTATLKANKSVGSYSGMVKERSTE
FVVNVSEAPTSVTGNVAGEDVTFRQVTTQEEYDAATDNVYFYNPNPSVIVKDYATEGTTY
ADIEDTTTPKLYVKSAEKVDITAYDFTVNVNGFKNEQDLGEDVLDESLAVPTGLAEVSKT
DAEITVAWDAVDDAVSYDIEVDGTVLRNITDTTYTQVDLSYLTNHTYRVRTVAANGHYSN
WSDLVTIQTADDPYRNVPTVTPTWEYGDSWGDLEGGFDHDTNTMFHSTNAVTPDQMLTLD
LGAAYQLDKLTYQPRMDNKGNGTVYRMDVYASLDGVNYTKVWDGNANEAWTYSSSMDVDD
IKEMPLNGVKARYLKLSVLSSRGGFFSASEITPYKLDGTDAWVVGDVNNSGAVDDNDLVF
YENYVGLKPVDSDWDYSTLGNIDNNDIIDAYDIAYVASMLGDEITNPASGVEGKIEIIPS
KTDIKAGDTVTLNYYGIGLKNVNAFSVEMPVDTDLFEVTNFGSASLQTVFMRNFSKTRFH
SDGSVDNYVCFTNVGNQDLINGTGSIAKVTIKANADFTWDTKATRAVVVGQDLSKADALI
DITQVPTAPETKNILGSSDFASITFSNDVKENMTGDELWQQSNWRDLLLDGDKGGTLAEF
KWYLTSYPESGDIAEEVKLPTDMNFEFNKAEPLKTVTVYNRTGGNGRVTSIKAVAYSGDT
EYDLGTYDSAQDEFVFTVPAEATNIDRVVITPMTSTGTATGTTTGSESNRMLSLREIEFV
TDSAVNATGIKFDESSASKVYVGGLAEVSATVSPNNASNPFYDITSSDESIAKVIKIPME
DTYIYAVQGISEGTVTLTATSEDGQFEATTELEVAVGVDTSVLDEQIAKFDSLYKNLYTT
ESYDQVKAAVDAAKELIASDEATQSDIDSATINIVNAINKLEFKGSNTDQPSSLNLIPQS
GMSRYDESSMSAVEKEDASYVIDGDKDTIWHSNYNSGYSLPQYVTIDLGAVYNLEQVDML
ARQNSRNGHITHYRIEVSTDEGENKIFTPVVEGYIANDGSSIEEPGVAKEIKFDTVEARY
VRFIAIESLGDRLNAYASIAELNFYGLAKDEVVETNKVALQIAVDTANTLKEQGALNNVV
PAVVAEFESALAEAETILADSSADQTTIDTSFYRLANAIHMLEFVKGDKSELDALINEAE
KYEEGNYTTDSWTALQEALDAAKDVMNDENALESDVNEALNNLKDAISNLVLSADKSRLQ
AKYDELTGLDSSLYTEASWPQLEEALANAKVVLDDSNATQTEVDNAYEALIRAQLSLRLI
PNKDLLQDLINKAETLNAANYTQESWGLFVQSLTAARSALGNENATQEEVAAAIEGLEAG
INSLVANDAATTPVASGDTTASINTGDSTSMMTSLAGLALASAMIYGAKKRKKSN

Enzyme Prediction     

No EC number prediction in MGYG000001697_01078.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH31	455	694	4.5e-56	0.5292740046838408
CBM32	1688	1822	3.4e-21	0.9112903225806451
CBM32	989	1110	7.8e-16	0.8548387096774194

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06596	GH31_CPE1046	3.18e-173	284	665	1	334	Clostridium CPE1046-like. CPE1046 is an uncharacterized Clostridium perfringens protein with a glycosyl hydrolase family 31 (GH31) domain. The domain architecture of CPE1046 and its orthologs includes a C-terminal fibronectin type 3 (FN3) domain and a coagulation factor 5/8 type C domain in addition to the GH31 domain. Enzymes of the GH31 family possess a wide range of different hydrolytic activities including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-xylosidase, 6-alpha-glucosyltransferase, 3-alpha-isomaltosyltransferase and alpha-1,4-glucan lyase. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein.
COG1501	YicI	4.32e-62	48	740	6	709	Alpha-glucosidase, glycosyl hydrolase family GH31 [Carbohydrate transport and metabolism].
pfam01055	Glyco_hydro_31	1.87e-52	267	694	1	442	Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases.
cd06589	GH31	1.00e-41	343	575	20	265	glycosyl hydrolase family 31 (GH31). GH31 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-xylosidase, 6-alpha-glucosyltransferase, 3-alpha-isomaltosyltransferase and alpha-1,4-glucan lyase. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. In most cases, the pyranose moiety recognized in subsite -1 of the substrate binding site is an alpha-D-glucose, though some GH31 family members show a preference for alpha-D-xylose. Several GH31 enzymes can accommodate both glucose and xylose and different levels of discrimination between the two have been observed. Most characterized GH31 enzymes are alpha-glucosidases. In mammals, GH31 members with alpha-glucosidase activity are implicated in at least three distinct biological processes. The lysosomal acid alpha-glucosidase (GAA) is essential for glycogen degradation and a deficiency or malfunction of this enzyme causes glycogen storage disease II, also known as Pompe disease. In the endoplasmic reticulum, alpha-glucosidase II catalyzes the second step in the N-linked oligosaccharide processing pathway that constitutes part of the quality control system for glycoprotein folding and maturation. The intestinal enzymes sucrase-isomaltase (SI) and maltase-glucoamylase (MGAM) play key roles in the final stage of carbohydrate digestion, making alpha-glucosidase inhibitors useful in the treatment of type 2 diabetes. GH31 alpha-glycosidases are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
cd06603	GH31_GANC_GANAB_alpha	6.60e-36	478	696	222	428	neutral alpha-glucosidase C, neutral alpha-glucosidase AB. This subgroup includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIK69254.1	0.0	45	2105	40	2258
QIK85989.1	0.0	8	2105	3	2115
QJA02122.1	0.0	1	2065	1	2116
BBK23937.1	0.0	4	2102	3	2193
QBJ76643.1	0.0	3	2114	4	2146

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6M76_A	2.45e-289	52	975	37	963	GH31alpha-N-acetylgalactosaminidase from Enterococcus faecalis [Enterococcus faecalis ATCC 10100],6M77_A GH31 alpha-N-acetylgalactosaminidase from Enterococcus faecalis in complex with N-acetylgalactosamine [Enterococcus faecalis ATCC 10100]
7F7R_A	1.34e-288	52	975	37	963	ChainA, GH31 alpha-N-acetylgalactosaminidase [Enterococcus faecalis ATCC 10100]
7F7Q_A	3.70e-288	52	975	37	963	ChainA, GH31 alpha-N-acetylgalactosaminidase [Enterococcus faecalis ATCC 10100]
6JR6_A	3.18e-31	52	698	30	692	Flavobacteriumjohnsoniae GH31 dextranase, FjDex31A [Flavobacterium johnsoniae UW101],6JR6_B Flavobacterium johnsoniae GH31 dextranase, FjDex31A [Flavobacterium johnsoniae UW101],6JR6_C Flavobacterium johnsoniae GH31 dextranase, FjDex31A [Flavobacterium johnsoniae UW101],6JR6_D Flavobacterium johnsoniae GH31 dextranase, FjDex31A [Flavobacterium johnsoniae UW101],6JR7_A Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose [Flavobacterium johnsoniae UW101],6JR7_B Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose [Flavobacterium johnsoniae UW101],6JR7_C Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose [Flavobacterium johnsoniae UW101],6JR7_D Flavobacterium johnsoniae GH31 dextranase, FjDex31A, complexed with glucose [Flavobacterium johnsoniae UW101]
6JR8_A	9.60e-31	52	698	30	692	Flavobacteriumjohnsoniae GH31 dextranase, FjDex31A, mutant D412A complexed with isomaltotriose [Flavobacterium johnsoniae UW101],6JR8_B Flavobacterium johnsoniae GH31 dextranase, FjDex31A, mutant D412A complexed with isomaltotriose [Flavobacterium johnsoniae UW101],6JR8_C Flavobacterium johnsoniae GH31 dextranase, FjDex31A, mutant D412A complexed with isomaltotriose [Flavobacterium johnsoniae UW101],6JR8_D Flavobacterium johnsoniae GH31 dextranase, FjDex31A, mutant D412A complexed with isomaltotriose [Flavobacterium johnsoniae UW101]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9F234	4.52e-34	75	740	47	713	Alpha-glucosidase 2 OS=Bacillus thermoamyloliquefaciens OX=1425 PE=3 SV=1
B9F676	1.41e-24	265	740	331	818	Probable glucan 1,3-alpha-glucosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0216600 PE=3 SV=1
Q9FN05	4.22e-24	469	740	566	820	Probable glucan 1,3-alpha-glucosidase OS=Arabidopsis thaliana OX=3702 GN=PSL5 PE=1 SV=1
Q4R4N7	1.29e-23	460	740	585	851	Neutral alpha-glucosidase AB OS=Macaca fascicularis OX=9541 GN=GANAB PE=2 SV=1
P79403	2.23e-23	460	740	585	851	Neutral alpha-glucosidase AB OS=Sus scrofa OX=9823 GN=GANAB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.042646	0.952924	0.003337	0.000451	0.000311	0.000300

TMHMM  Annotations     

start	end
9	31