CAZyme Information: MGYG000001704_01029

Basic Information

• Species: Blautia hansenii
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia; Blautia hansenii
• CAZyme ID: MGYG000001704_01029
• CAZy Family: GH101
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2146	MGYG000001704_1|CGC19	237051.5	4.6359

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001704	3065949	Isolate	not provided	not provided

• Gene Location: Start: 1072320; End: 1078760 Strand: +

Full Sequence      

MKKRNMKVFSLLLAASMTVPQLADYSQLSGLTVVHAAVNEQSSEKAVTEVWRHFTSGATN
DNAKKPAVLKNTNENAIDPAGGEISLVLKTSQNSALNRIQICPYWVDGGNFINAAGFDAS
SKWFFEYKADGLSGNWPGLGVSEWNANEEVPIVISWKENVYTVTIKGQKCAEQTVPATFI
EALKGGSFAIKAGTWGEEITDIYFKDVQIKNSKGEVVVEKGADTWEVSTGNGEAFEAGKE
VTTVSLTGKVVDKEKNPVAGATVTVGDKSATTGEDGSWALEGVEPGEVEIVVTKEGYKST
TTRATIEKEDKVIDDITLSTGDAVDFEDAADESISSDEMKVAIDSKFPRVAGYEILKGNG
AGKKMYGQTEKLDTIVLNQTKADDKTGIAVKPEVTSEKNKEGNKMVYTMKVDDKENNIHA
TIKAELVAEKNTLAFNITSVEREKGSELVKTINIPNHNLVSVRTSQAGARFDGANMSNNT
KQSGDTHQDVASMAAGKTGYMYAFVSDADLSAGLWSNSENNVNRDWQRVVANAAEKDGYR
ETGLSSNYWTWQKGEDYREESKDYELPSTKIAITADENNDAKVDWQDGAIAYRDIMNNPK
GSELVPDRTAIRIAMNFSSQAQNPFLMTLDNVKKVYLNTDGLGQSVLLKGYGSEGHDSGH
LNYADIGTRIGGAEDMTTLMKEGAKYGATFGIHVNASETYPESIYFEEDRLMKGSNGKYS
YGWNWLDQGININADYDLKHGREQRFLDLKEKLDKADPDGKNDLDFIYVDVWGNGQSGDN
GTWASRQLAKEITETCDWRLAGEWGHANEYDSTFQHWAADLTYGGAANKGINSAITRFIR
NHQKDSWVGDYPSYGGAAVNPLLGGYDMKDFEGWQGRNDYKGYIENLFDDDVSTKFLQHY
EVMQWVDGNPTTVADSTWTPEMKVVLQDKERKQTITVERQSNNGSDAGYSLRTMKFDDGN
GERVIMDGEKYLIPWYWDANGESLGDNYKLYHWNQAGGTSTWTLPAGWENAKVYELTENG
KKEVTNATVSGGQITIDATAKTPYVLYKTEAKNPTNEELKWSEGTHLVDTGFNSASLEQW
KISGEAEAAQIIHSAGFNPMLRLNNQTDEVVLEQKAKGLEKGKQYVAMVAVDNRSDAKAY
LEVTSGDKTTSNYTTKSIAKNYSQADAHNTNASNATIQGQGSYFQNMYVFFTAGEDGTAT
IKLKRDKGEGEVYFDDLRIVQNNSKNFVSDNKFVQNFETVPQGLWPFVVGGAEGVTDNRT
HLSEKNKKDSKYTSAGWFTKKLDDVLEGDWSLKTNGLTQRGNLIYQTIPQNFRFEEGVTY
NVSFDYQCGSEGTYALAVGNGEVEDGNVEYTPLESSIPKGQKAETKHYKFRITGSEGGQS
WFGIQSTTKGPDLQNTSGGDADFGGYKDFVLDNLVIEKSKAQKVKLETLVAENQGRYEVN
YSAKTWKTFTEAMKNANEALDNFDADQATVDKAYEKLDKAIKGLEVIGATMSGTVTDTEG
NPIENITVSVKMKDKTIKTVTDSNGQYVLPGVLFGDWKVTADSPIWAKATADIKSTKDNL
EFTQDFQLKDTQSSLEGHVTAVGKNVEDATVSLTGKSEVEAIKTDAKGAYKFDKLLAGNY
TLKVEKEGYDTFTQEITVTKEKGFVQNVMLQPASTVDYENDYSNGKKTWDNLAGNGSSTT
ISVENEATKIKFPGGGHANVYETAAPTFKNGVVEMDVTPEKSGIRLGVLLRANNMNDRVY
IGVGDADNQWFAEYWGSDGSNSWSSMHSGPKATAGKKLHLKAEIIETTVSLWVDGELVIN
KLNMGGMPTKAGAVGMNTRNSNTVTVDNVKVTSYDLPVGEVETVAGHVSDKDGNVLEGVS
VNIKSAEGAAEVVDKTAKTDALGNYKFKNIPLGKYKVTATNDKETKTVDVTVKATDDYIV
APEICFGVTTDGWKETTDGWEYYEDGKKVTGWKEISKHWYYFESNGIMTTGWTEVGEHWY
YMDQWGAMMTGWVSVDGHWYYMDQWGAMTTGWVYVNGHYYYMDRWGAMQTGWVSVDGHWY
YLNADGAMATGWVYVNGHYYYMDQWGAMQTGWVSVGGHWYYLNADGAMATGWVYVGGYWY
YLDQWGAMTTGWVQVGNDWYYMNSDGTMATNQWIGGYYVGASGKME

Enzyme Prediction     

EC	3.2.1.97

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH101	336	1048	9.3e-287	0.9971711456859972

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12905	Glyco_hydro_101	3.13e-146	586	884	1	273	Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
cd14244	GH_101_like	1.90e-117	599	907	1	298	Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam17974	GalBD_like	9.73e-91	1215	1405	1	190	Galactose-binding domain-like. Proteins containing a galactose-binding domain-like fold can be found in several different protein families, in both eukaryotes and prokaryotes. The common function of these domains is to bind to specific ligands, such as cell-surface-attached carbohydrate substrates for galactose oxidase and sialidase, phospholipids on the outer side of the mammalian cell membrane for coagulation factor Va, membrane-anchored ephrin for the Eph family of receptor tyrosine kinases, and a complex of broken single-stranded DNA and DNA polymerase beta for XRCC1. The structure of the galactose-binding domain-like members consists of a beta-sandwich, in which the strands making up the sheets exhibit a jellyroll fold.
pfam18080	Gal_mutarotas_3	5.12e-83	334	585	1	243	Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
NF033930	pneumo_PspA	6.37e-76	1932	2143	444	657	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ASM68979.1	0.0	1	1969	1	1969
QWG66803.1	0.0	334	1417	56	1161
QHH84546.1	0.0	334	1426	56	1169
QWG33197.1	0.0	334	1417	56	1161
ADY21335.1	0.0	334	1417	56	1161

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6QFK_A	0.0	332	1477	10	1180	EngBFDARPin Fusion 4b G10 [Bifidobacterium longum]
6QEP_A	0.0	330	1477	8	1180	EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
6QEV_B	0.0	332	1477	10	1180	EngBFDARPin Fusion 4b B6 [Bifidobacterium longum]
5A59_A	0.0	320	1418	5	1102	Thestructure of GH101 E796Q mutant from Streptococcus pneumoniae TIGR4 in complex with T-antigen [Streptococcus pneumoniae TIGR4],5A5A_A The structure of GH101 E796Q mutant from Streptococcus pneumoniae TIGR4 in complex with PNP-T-antigen [Streptococcus pneumoniae TIGR4]
6QFO_A	0.0	330	1477	8	1180	EngBFDARPin Fusion 9b 3G124 [Bifidobacterium longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q2MGH6	0.0	320	1473	320	1474	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1
Q8DR60	0.0	320	1473	320	1474	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1
A9WNA0	2.25e-115	329	1417	47	1035	Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2
Q6GBS5	2.01e-07	948	1642	323	1095	Serine-aspartate repeat-containing protein D OS=Staphylococcus aureus (strain MSSA476) OX=282459 GN=sdrD PE=3 SV=1
Q8NXX6	3.43e-07	948	1642	323	1095	Serine-aspartate repeat-containing protein D OS=Staphylococcus aureus (strain MW2) OX=196620 GN=sdrD PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000676	0.998482	0.000260	0.000228	0.000174	0.000158

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001704_01029.