CAZyme Information: MGYG000001709_00473

Basic Information

• Species: Collinsella stercoris
• Lineage: Bacteria; Actinobacteriota; Coriobacteriia; Coriobacteriales; Coriobacteriaceae; Collinsella; Collinsella stercoris
• CAZyme ID: MGYG000001709_00473
• CAZy Family: GH20
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
569		61866.12	5.3837

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001709	2399821	Isolate	Japan	Asia

• Gene Location: Start: 11627; End: 13336 Strand: +

Full Sequence      

MYKMKHGMKRVLAGVVAAVFAVTCLVALPARAYAAGGHSIFSIDAGRKYFSADQLKQIID
RAAKNGYTDVQLILANDGMRFFLDDMSIEADGKVYESDAVKAALTAGNDSYYKDPNGNAL
TEAEMNDIISYAKAKGVGIVPVINSPGHMDALLVAMKELGIENAAYSNSDRVSVRTVDIT
NQKAVNFLYAMLQKYVTYFGNAGASEYFNFGADEFANDVFSDPGWGEIQRNGEYKNFIEY
ANHVCGIIKDAGMKPLCFNDGIYYNQKTNFGAFDQDLTVSYWTSGWWGFYVAKPEFLQQQ
GLEILNTNDAWYWVLGHISDGGYHFNNAMSNIEKKGFNEVTGASGTIPTIGSMQCVWCDE
PSKPHDMERIFQLMDAFSAKHAEHMPKPADYAKVDEALATVPEDLSIYTPESRKAVEDAV
AAVKRGYKADKQAEVDKMAADINAAIDALAFRPADYSAVDAALARVPKDLTGYTDATRVT
VQNAVASVKRGYTVDKQADVDAMAAAINAAVDGLVKKPVVKPEQKPSSKPVLKPSEKLPQ
TGDPVQILAVASAGLSVLGMGLLASRRKA

Enzyme Prediction     

EC	3.2.1.52	3.2.1.96

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH20	41	372	3.6e-45	0.9080118694362018

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06564	GH20_DspB_LnbB-like	5.79e-98	38	381	2	323	Glycosyl hydrolase family 20 (GH20) catalytic domain of dispersin B (DspB), lacto-N-biosidase (LnbB) and related proteins. Dispersin B is a soluble beta-N-acetylglucosamidase found in bacteria that hydrolyzes the beta-1,6-linkages of PGA (poly-beta-(1,6)-N-acetylglucosamine), a major component of the extracellular polysaccharide matrix. Lacto-N-biosidase hydrolyzes lacto-N-biose (LNB) type I oligosaccharides at the nonreducing terminus to produce lacto-N-biose as part of the GNB/LNB (galacto-N-biose/lacto-N-biose I) degradation pathway. The lacto-N-biosidase from Bifidobacterium bifidum has this GH20 domain, a carbohydrate binding module 32, and a bacterial immunoglobulin-like domain 2, as well as a YSIRK signal peptide and a G5 membrane anchor at the N and C termini, respectively. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
COG3525	Chb	3.34e-30	42	418	16	381	N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism].
cd02742	GH20_hexosaminidase	4.07e-28	41	377	4	297	Beta-N-acetylhexosaminidases of glycosyl hydrolase family 20 (GH20) catalyze the removal of beta-1,4-linked N-acetyl-D-hexosamine residues from the non-reducing ends of N-acetyl-beta-D-hexosaminides including N-acetylglucosides and N-acetylgalactosides. These enzymes are broadly distributed in microorganisms, plants and animals, and play roles in various key physiological and pathological processes. These processes include cell structural integrity, energy storage, cellular signaling, fertilization, pathogen defense, viral penetration, the development of carcinomas, inflammatory events and lysosomal storage disorders. The GH20 enzymes include the eukaryotic beta-N-acetylhexosaminidases A and B, the bacterial chitobiases, dispersin B, and lacto-N-biosidase. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by the solvent or the enzyme, but by the substrate itself.
COG3525	Chb	9.21e-26	42	373	429	732	N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism].
cd06565	GH20_GcnA-like	4.20e-15	53	282	17	210	Glycosyl hydrolase family 20 (GH20) catalytic domain of N-acetyl-beta-D-glucosaminidase (GcnA, also known as BhsA) and related proteins. GcnA is an exoglucosidase which cleaves N-acetyl-beta-D-galactosamine (NAG) and N-acetyl-beta-D-galactosamine residues from 4-methylumbelliferylated (4MU) substrates, as well as cleaving NAG from chito-oligosaccharides (i.e. NAG polymers). In contrast, sulfated forms of the substrate are unable to be cleaved and act instead as mild competitive inhibitors. Additionally, the enzyme is known to be poisoned by several first-row transition metals as well as by mercury. GcnA forms a homodimer with subunits comprised of three domains, an N-terminal zincin-like domain, this central catalytic GH20 domain, and a C-terminal alpha helical domain. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QMW75670.1	9.98e-204	30	568	27	561
QPS13996.1	9.98e-204	30	568	27	561
QQY27185.1	9.98e-204	30	568	27	561
QQV05864.1	9.98e-204	30	568	27	561
AYV34742.1	5.50e-180	39	501	37	497

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7PUL_A	1.54e-143	39	385	11	350	ChainA, Beta-N-acetylhexosaminidase [Enterococcus faecalis]
4AZ7_A	1.41e-129	40	424	9	402	Differentialinhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH [Streptococcus pneumoniae TIGR4]
2YL6_A	1.46e-129	40	424	9	402	Inhibitionof the pneumococcal virulence factor StrH and molecular insights into N-glycan recognition and hydrolysis [Streptococcus pneumoniae TIGR4],4AZ5_A Differential inhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH [Streptococcus pneumoniae TIGR4]
4AZ6_A	1.51e-129	40	424	11	404	Differentialinhibition of the tandem GH20 catalytic modules in the pneumococcal exo-beta-D-N-acetylglucosaminidase, StrH [Streptococcus pneumoniae TIGR4]
2YLL_A	2.81e-129	40	424	29	422	Inhibitionof the pneumococcal virulence factor StrH and molecular insights into N-glycan recognition and hydrolysis [Streptococcus pneumoniae TIGR4]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P49610	1.54e-116	38	449	632	1041	Beta-N-acetylhexosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=strH PE=1 SV=2
B2UPR7	1.50e-07	41	365	237	544	Beta-hexosaminidase Amuc_2136 OS=Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc) OX=349741 GN=Amuc_2136 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000233	0.999064	0.000171	0.000183	0.000168	0.000143

TMHMM  Annotations     

start	end
12	34