CAZyme Information: MGYG000001712_02657

Basic Information

• Species: Bacillus_A thuringiensis_S
• Lineage: Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae_G; Bacillus_A; Bacillus_A thuringiensis_S
• CAZyme ID: MGYG000001712_02657
• CAZy Family: GT2
• CAZyme Description: Tyrocidine synthase 3

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2338		269410.3	5.0645

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001712	5736823	Isolate	United States	North America

• Gene Location: Start: 2629146; End: 2636162 Strand: -

Full Sequence      

MHIKDRSSIHENKEVEAFWKEQLSSEILDFSVFKFNSDYQLNKETPCEYVQVRVNVEFSK
SIQKISKSQDLLLFSWFQAALRVCLAHYTDQERITIGIPIIEKNLEQSENLNKLIPLGSE
IHPHHPFKQLVNEIEQTTLFGYENQEYPLSELLVKHNLTPFQIVIGMEGLHNENSLEEYA
ENNLVLWIRRKWNEIHNEFEFQISCKSRSFSSLQQFANSYCYVLKQVSQNPNLALKDICI
IAEEEKELLEGLNTFQTGIDLFQSFQDLFEAQVLKTPDQIAVVCNDQSLTYRELNKKANQ
LASILQSKGVSKESIIGVMVDRSLEMIIGMMGILKAGAAYLPIDPNYPTERIEYMLQDSQ
AKYLLSKRTEEVLPQFAGEVLYFDKEYLFQGEESNLVREHNPNDLAYVIYTSGSTGNPKG
VMIEQKSLVHFLNVMREKVKANQSFLFLASVSFDISLLEICLPLTQGSKVVIATEEQFAT
KELAHLVKKHKVDLWESTPSRMEVILSDPEGANFLKDLKSILLAGEAFSIDLVEKIRCIS
EATILNIYGPTETTICATIKDLSTSKEVTIGKPNPNYHSYILNKYGQLKPFGIPGELCIA
GVAVARGYLGKSKLTDEKFVQSPFAAGEMMYHTGDLVRWLPNGELEYLGRMDHQVKIRGY
RIELREIETQLREYPEINQAIVVDQVYGNRKLLAAYYVSDNKVSFGEIRKYLSDKLPEFM
IPEKMIQVEEIPLNPNGKVDRKRLLDITQTDYVSIPYVAPRNEIEQKLVRAWEKVMEIEG
IGIHDNFFALKGESIKALQVINLLRKDCLKISTTDFFKHPTIAQLSSCVKLEHKEENFES
KPTHVKDLSNPFSLTEVQTAYMLGRNSQFELSGISPQTYFEYETTLDINRLSKSFQKVIH
RHPMLRAVILPEGKQQILQSVPDYQIEIVSLIDLDDRNQNARLQEERSRMTNHVFPLGQW
PLFELKAFLLKEDTYLLCFRYDALLMDGASMNIVGQDLLHYYYKPNQKLESLSFDFQDYM
FIYDEMEQIGEYKTAKDYWTSKLPDFPFAPSLLLKKDPAEIATPKFQSLTKILDNEKWTK
LRKLAQEKEVTPSALLCTIYGDVLAYWSNQRRLAINLTVFNRYPVHDEVEQIVGDFTSLI
LLDVDVKPEQTFFTRVKETQSTLLDGLEHRHYDGVNFIRDFTRYHQMTPKAVMPIVFTSM
LAGAGAFSWEQLGSLRYIHARTPQVYLDNVVIEKNGELLISWNYVEELFDIDVIEAMFSQ
FVDLLEQLVKQSDITSLQMKESDQTLIEQYNETTEKIPSTTLYQLFTDQVKRTPNEVAVV
FEQKWLTYSELHKRSNQIAHFLKEQGIGLGDKVGLLAKRRVDTIVNMLGILKAGAAYVPI
DPDHPLDRQTYILKNSSCKFLLEPSLYEENDLSFYTTEDMPAIAGPEDIAYIIYTSGSTG
KPKGVIITHQAVTNTIQDINQKYEVNEDDRIISISSMCFDLSVYDIFGALSTGAMLVMIR
DLRDMQELIRTVERRGITIWNTVPAIMDLALDQVGSHFEHSSLRLVLHSGDWIPLSLPEK
IKRHFPIAEVVSLGGATEASIWSIYYPVKQVESHWNSIPYGMPLANQTYYVLNYEKKMCP
VGVIGDLYIGGVGLAKGYLNDEKKTNEAFVSHPDFGLIYKTGDCGKMHSEGYIEFLGRQD
YQVKIQGYRVELEEISHCLLTYKQVEHAVVIDQTDENGIKFLVAYVVTEQNISTTELRKH
LRDHLPDYMIPSYFVYLDQLPLTPNGKLDRKALPTPEKQKNEVFIAPKTEMEKILTSIWQ
EVLKIDQVGVNDHFFALGGDSIKAIQVSVRLYRQGFELEPKNLFSFPILRDMVQFVKKLD
QFSSSQTNDVNIMNDNKATLLNVKDKQLNQHTLTKIQEKMPDIKIERIYPLAPFQEVIYE
HATNEPDTNAYFEQTIWALEGELDIKLFIQCFQQLVDRHDSLRTIIVQDEQAKPWQAVLY
DVQINSEIKNLIEMTKQEQQEFLDQWMNENLSQGFKNGELMTRLCIFQLGNNEYKVIWSS
HHLLCDGWSVSILIDELFQLYETTNAGTTAELPMVKPFETFIDWTLAQDHEKARHFWRDY
LSGYNQKISIPKKKVPSKSEGLNFTFMDLTLDKDLTNQLQTFVTKNNITMNTALLAVWGI
LLGKFNGTREVVLPNLVSVRPPEVEGIENMFGLFTNVLPIRLAWTETQSFVDFLQKVQLE
TLKCNEYAYYSFVDIQKQSELKTELTDHLWVFENYPTNPAIFSSNENRHFAITDYEVVDE
PHVKYGIMCFPEERLTIKFGYDQTVYEAEKIQEILNHIHGLINTILQNPIQTICDYKL

Enzyme Prediction     

No EC number prediction in MGYG000001712_02657.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12467	PRK12467	0.0	17	2202	241	2474	peptide synthase; Provisional
PRK12467	PRK12467	0.0	885	2218	83	1427	peptide synthase; Provisional
cd17655	A_NRPS_Bac	0.0	268	744	2	486	bacitracin synthetase and related proteins. This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
cd12114	A_NRPS_TlmIV_like	0.0	1314	1773	1	477	The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
PRK05691	PRK05691	0.0	17	2203	867	3084	peptide synthase; Validated

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QND46664.1	7.80e-230	230	1858	975	2664
BAY30132.1	2.69e-123	760	1827	2144	3264
BAZ00088.1	3.54e-123	760	1858	2142	3294
BAZ75991.1	3.54e-123	760	1858	2142	3294
BAY90071.1	6.11e-123	716	1827	2081	3253

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7LY7_A	0.0	847	1859	6	1019	ChainA, BmdB, Bacillamide NRPS [Thermoactinomyces vulgaris]
7LY4_E	0.0	847	1859	6	1019	ChainE, BmdB, bacillamide NRPS [Thermoactinomyces vulgaris]
6MFZ_A	3.70e-265	263	1856	205	1797	Crystalstructure of dimodular LgrA in a condensation state [Brevibacillus parabrevis],6MFZ_B Crystal structure of dimodular LgrA in a condensation state [Brevibacillus parabrevis]
6MFY_A	1.78e-243	263	1777	205	1716	Crystalstructure of a 5-domain construct of LgrA in the substrate donation state [Brevibacillus parabrevis],6MG0_A Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis],6MG0_B Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis]
5T3E_A	8.87e-239	847	1289	3	445	Crystalstructure of a nonribosomal peptide synthetase heterocyclization domain. [Thermoactinomyces vulgaris],5T3E_B Crystal structure of a nonribosomal peptide synthetase heterocyclization domain. [Thermoactinomyces vulgaris]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O68006	0.0	241	2328	15	2091	Bacitracin synthase 1 OS=Bacillus licheniformis OX=1402 GN=bacA PE=3 SV=1
O30409	4.68e-318	57	2338	1272	3551	Tyrocidine synthase 3 OS=Brevibacillus parabrevis OX=54914 GN=tycC PE=1 SV=1
O68008	2.80e-317	11	2338	187	2976	Bacitracin synthase 3 OS=Bacillus licheniformis OX=1402 GN=bacC PE=3 SV=1
P0C064	2.43e-307	16	2338	1238	3564	Gramicidin S synthase 2 OS=Brevibacillus brevis OX=1393 GN=grsB PE=1 SV=2
P0C063	2.33e-300	16	2338	1238	3565	Gramicidin S synthase 2 OS=Aneurinibacillus migulanus OX=47500 GN=grsB PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000051	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001712_02657.