CAZyme Information: MGYG000001720_01946

Basic Information

• Species: Prevotella sp002350355
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella sp002350355
• CAZyme ID: MGYG000001720_01946
• CAZy Family: PL11
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1518		163282.94	5.4417

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001720	3435079	MAG	Sweden	Europe

• Gene Location: Start: 12921; End: 17477 Strand: -

Full Sequence      

MRKKYILVGLLAMFLTAGTHAQRVTDRLDRGLVAVKTSGGVFCSWRINAEEYYAVTYNLY
RDGVKVNDAPLTVSNYTDPAGTTSSTYTVRPIIKGVEQTAGSKPAAVLANDYLEIAKPKR
LSNDGKTDVTDVYQPNDATIADVDGDGEMELIVKELCTTDDPKNKPDGPDFDRIEVFKLN
GDLLWWIDCGPNLCDFQHNETNIAAYDWDGDGKAECIMRAADGTILHTATGKTIVVGDKS
KNYRADCQNGSMAEFFVHSGAEYLLYLNGQTGEPYQIGPSEHPNYMSYPLKRLEDGETDL
KKAWGDGYGHRSSKNFFGAPYLDGHKPSIFLARGIYTRHKMIALDVDPATHHLSTRWRWT
CNTPGSPWYGQGYHNYTIADVDWDGRDEICFGSMVIDDNGKGLSTTGFGHGDAQHVGDFD
PYRHGQEIFACNEDAPANNYRDATTSKIYYRLAGGGDDGRSMMGNFTDDVPGAIGASGHD
TPISAVTAKHTDNTSKDFDLNFRIYWDGDLLEETLNGTQTRNSTFRIHKYGAGDIKTLDG
SLSNNDTKATPCFQGDIFGDWREEVVARTPDNKIRIYTTTDPTEYRNCTLLSDKQYRNAM
VWQMNGYNQPPHVSYFLGKMEGITMAPPPETTNGRTVVNNGGSIGTRLNDKQVLLDENND
MTVSVSDGAAPYIFFDNAPTWVQGHDDNNNITTTVYTHTLTGGAFSGAMRLIKQGDGTLV
LPKVDEKYTGNTDVWAGTLVFDGTLKSSRLWLNRFANLHSDGGTFSAIKADYGAAVRPGG
QDKVGTITADTLKLGFGSIVDFDIDASSNADQIVVNKVMSIEQKDWTQGPEYLVPRFNFH
FIDGNVKAGSYMLGKVQKVDGDISKVAVTGLNGHKAALSVTDGNLVLTIAGMRQAAGVEW
SGAQSSMWDIADAENFTNTSEKTSDVFVSGDHVTFNDNAASSTVVIKGEVSPASVTFDNS
KLEYDLSGDPITGNTTITKKGSATATLHNVNDFTGSVNIENGTLAVDNLGQKSGVNNGAL
GVYTNKVTLNGGTLETLATLTADHPVVLGAKGGTILVDEGKTLTLNAGITGNGNVLTKAG
KGTLTLAPSANYGTLNVMAGWVNSQEQDNVHGYPKNIVLNNGTTLSDADNIYSYSTNGVN
VKVPEGATASWYLDSRCNYTGQLTGGGTLNLYSRSTRAYLKGYWGDFTGRLNIAGKKTGS
YDPMLYVQGSLKNAEINATITLNNNGSNVAVGSLSGSAELDGKGTWSVGGNDKDFTFQGY
INGGGLIKIGNGMMTIGKKQDKLGKQVDVQGGTLNINSSATTVTADNRWFGSQSVSVTGG
GCFAGRGVVEKILVNDGGTLQPGNAANAIQYGALKTANGCYLYKGAKVLFNIYSTKFYPS
LATNGTLSLADGSTVAVTLSSKYTPKVGDTFTLWDAAMLNATADGVTLQLPTLPNGFAWD
TSELFQQKGLLKVVAATGINQLTADTVFHCEVFKTNGARVGVLTTTKARLNEDIRGLHVE
PGLYLVKVGATTMKVLVK

Enzyme Prediction     

No EC number prediction in MGYG000001720_01946.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL11	25	620	2.8e-159	0.9759862778730704

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd10318	RGL11	2.61e-170	26	615	2	564	Rhamnogalacturonan lyase of the polysaccharide lyase family 11. The rhamnogalacturonan lyase of the polysaccharide lyase family 11 (RGL11) cleaves glycoside bonds in polygalacturonan as well as RG (rhamnogalacturonan) type-I through a beta-elimination reaction. Functionally characterized members of this family, YesW and YesX from Bacillus subtilis, cleave glycoside bonds between rhamnose and galacturonic acid residues in the RG-I region of plant cell wall pectin. YesW and YesX work synergistically, with YesW cleaving the glycoside bond of the RG chain endolytically, and YesX converting the resultant oligosaccharides through an exotype reaction. This domain is sometimes found in architectures with non-catalytic carbohydrate-binding modules (CBMs). There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain through a beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
pfam18370	RGI_lyase	3.25e-36	23	107	1	85	Rhamnogalacturonan I lyases beta-sheet domain. This is the beta-sheet domain found in rhamnogalacturonan (RG) lyases, which are responsible for an initial cleavage of the RG type I (RG-I) region of plant cell wall pectin. Polysaccharide lyase family 11 carrying this domain, such as YesW (EC:4.2.2.23) and YesX (EC:4.2.2.24), cleave glycoside bonds between rhamnose and galacturonic acid residues in RG-I through a beta-elimination reaction. Other family members carrying this domain are hemagglutinin A, lysine gingipain (Kgp) and Chitinase C (EC:3.2.1.14).
PRK15319	PRK15319	1.32e-09	707	1206	621	1134	fibronectin-binding autotransporter adhesin ShdA.
PRK09752	PRK09752	2.50e-04	963	1127	314	502	AIDA-I family autotransporter YfaL.
PRK15319	PRK15319	0.001	971	1240	747	1061	fibronectin-binding autotransporter adhesin ShdA.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIM09959.1	0.0	13	1508	2	1528
AGB29439.1	0.0	1	1515	1	1516
BCS86257.1	3.67e-311	24	898	1	875
ABN51485.1	6.91e-131	23	630	257	813
QYR22020.1	1.67e-130	19	633	48	644

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2Z8R_A	8.01e-127	21	629	1	578	Crystalstructure of rhamnogalacturonan lyase YesW at 1.40 A resolution [Bacillus subtilis],2Z8R_B Crystal structure of rhamnogalacturonan lyase YesW at 1.40 A resolution [Bacillus subtilis],2Z8S_A Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid [Bacillus subtilis],2Z8S_B Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid [Bacillus subtilis],2ZUX_A Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose [Bacillus subtilis],2ZUX_B Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose [Bacillus subtilis]
4CAG_A	1.08e-120	19	629	4	585	Bacilluslicheniformis Rhamnogalacturonan Lyase PL11 [Bacillus licheniformis]
2ZUY_A	7.38e-118	22	629	5	599	Crystalstructure of exotype rhamnogalacturonan lyase YesX [Bacillus subtilis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O31526	3.96e-126	2	629	11	615	Rhamnogalacturonan endolyase YesW OS=Bacillus subtilis (strain 168) OX=224308 GN=yesW PE=1 SV=1
O31527	3.20e-117	22	629	5	599	Rhamnogalacturonan exolyase YesX OS=Bacillus subtilis (strain 168) OX=224308 GN=yesX PE=1 SV=1
A0A0H3GGE2	2.24e-06	971	1087	2796	2902	Adhesin BmaC autotransporter OS=Brucella suis biovar 1 (strain 1330) OX=204722 GN=bmaC PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000470	0.998183	0.000744	0.000214	0.000195	0.000178

TMHMM  Annotations     

start	end
5	22