CAZyme Information: MGYG000001732_00561

Basic Information

• Species: HGM11530 sp900751685
• Lineage: Bacteria; Firmicutes_A; Clostridia; Monoglobales_A; UBA1381; HGM11530; HGM11530 sp900751685
• CAZyme ID: MGYG000001732_00561
• CAZy Family: GH42
• CAZyme Description: Beta-galactosidase BgaA

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
679	MGYG000001732_11|CGC1	78539.81	5.1528

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001732	3073889	MAG	Sweden	Europe

• Gene Location: Start: 15049; End: 17088 Strand: +

Full Sequence      

MKKRLDMDHITLGVCYYPEHWDKSLWRDDLKRMKEYGIEVIRIAEFAWNKIEPYEGEFTF
DFFDEFMDMALEENMKVIFCTPSATPPVWMSQKYPEILNADIDGNLIHHGLRRHCNLNSE
KYRFFVSRITEEMAGHYSKYPNIIAWQLDNEINCECDLYYSESDHKAFRQYLKNKFGTLD
QFNEAVGAVFWNQTYTSWAEVYLPRRTNAGRMGNPHVGLLEKRFISETVVNFFKLQADII
RRYTKAYITTNGLFGHIDYQRLAGEVLDFITYDNYPNFAYERRIDVSCQNGLRDRNSSYN
LTRARGISPIFGIMEQQTGPSGWNFRMEQPAPKPGQVRLWTLQAIAHGADYISYFRWRTC
TFGNEMYWHGLLDYSNQPNRRTAELERTCRDIQKIQLVCGEEYIAEAALLKDYDNEWDAE
ADVWHSEADKISYDGWFRAFQKKHVPYDLVYIDDDTALSELLKYKMVVYPHPAIFTQKRA
ALLRAYAEQGGTVIFGCRSGYKDLNGICYSMPMPGYAAELCGAEVEEFTFLSPYDEEQYV
RIGEKRVSAQCFNDVLKITGGESIGVYENNYYSGKPAVSVKNIGNGKVYYFGAAFGEDAA
KAFIDLEGIASPKGLEQFIKLPEQIELAVRGEYIFLLNYDSQAVEVGCLGVFKDIISGAE
YSDKIRINGYDGAVLIKKD

Enzyme Prediction     

No EC number prediction in MGYG000001732_00561.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH42	15	393	8.1e-119	0.9946091644204852

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam02449	Glyco_hydro_42	2.86e-124	15	395	1	376	Beta-galactosidase. This group of beta-galactosidase enzymes belong to the glycosyl hydrolase 42 family. The enzyme catalyzes the hydrolysis of terminal, non-reducing terminal beta-D-galactosidase residues.
COG1874	GanA	1.71e-97	6	675	12	670	Beta-galactosidase GanA [Carbohydrate transport and metabolism].
pfam08532	Glyco_hydro_42M	2.59e-34	405	616	1	207	Beta-galactosidase trimerisation domain. This is non catalytic domain B of beta-galactosidase enzymes belong to the glycosyl hydrolase 42 family. This domain is related to glutamine amidotransferase enzymes, but the catalytic residues are replaced by non functional amino acids. This domain is involved in trimerisation.
cd03143	A4_beta-galactosidase_middle_domain	3.67e-16	407	531	1	123	A4 beta-galactosidase middle domain: a type 1 glutamine amidotransferase (GATase1)-like domain. A4 beta-galactosidase middle domain: a type 1 glutamine amidotransferase (GATase1)-like domain. This group includes proteins similar to beta-galactosidase from Thermus thermophilus. Beta-Galactosidase hydrolyzes the beta-1,4-D-galactosidic linkage of lactose, as well as those of related chromogens, o-nitrophenyl-beta-D-galactopyranoside (ONP-Gal) and 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal). This A4 beta-galactosidase middle domain lacks the catalytic triad of typical GATase1 domains. The reactive Cys residue found in the sharp turn between a beta strand and an alpha helix termed the nucleophile elbow in typical GATase1 domains is not conserved in this group.
pfam08533	Glyco_hydro_42C	4.10e-04	630	675	11	56	Beta-galactosidase C-terminal domain. This domain is found at the C-terminus of beta-galactosidase enzymes that belong to the glycosyl hydrolase 42 family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNO17813.1	3.80e-267	3	678	21	700
QBE95795.1	4.39e-249	8	674	10	681
QIB55815.1	1.25e-248	8	674	10	681
QMW76319.1	1.25e-248	8	674	10	681
AEF83220.1	2.69e-247	1	674	1	679

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6LVW_A	5.05e-95	10	646	1	644	PolyextremophilicBeta-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi [Halorubrum lacusprofundi ATCC 49239]
6Y2K_A	9.08e-83	10	644	1	629	ChainA, beta-galactosidase [Marinomonas sp. ef1]
1KWG_A	1.68e-82	12	645	2	618	Crystalstructure of Thermus thermophilus A4 beta-galactosidase [Thermus thermophilus],1KWK_A Crystal structure of Thermus thermophilus A4 beta-galactosidase in complex with galactose [Thermus thermophilus]
3TTS_A	4.83e-79	10	667	9	664	ChainA, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTS_B Chain B, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTS_C Chain C, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTS_D Chain D, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTS_E Chain E, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTS_F Chain F, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTY_A Chain A, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTY_B Chain B, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTY_C Chain C, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTY_D Chain D, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTY_E Chain E, Beta-galactosidase [Niallia circulans subsp. alkalophilus],3TTY_F Chain F, Beta-galactosidase [Niallia circulans subsp. alkalophilus]
5E9A_A	5.36e-75	13	678	42	709	Crystalstructure analysis of the cold-adamped beta-galactosidase from Rahnella sp. R3 [Rahnella sp. R3],5E9A_B Crystal structure analysis of the cold-adamped beta-galactosidase from Rahnella sp. R3 [Rahnella sp. R3],5E9A_C Crystal structure analysis of the cold-adamped beta-galactosidase from Rahnella sp. R3 [Rahnella sp. R3],5E9A_D Crystal structure analysis of the cold-adamped beta-galactosidase from Rahnella sp. R3 [Rahnella sp. R3],5E9A_E Crystal structure analysis of the cold-adamped beta-galactosidase from Rahnella sp. R3 [Rahnella sp. R3],5E9A_F Crystal structure analysis of the cold-adamped beta-galactosidase from Rahnella sp. R3 [Rahnella sp. R3]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
D9SM34	8.29e-104	10	642	1	622	Beta-galactosidase BgaA OS=Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B) OX=573061 GN=bgaA PE=1 SV=1
Q9RFN0	3.96e-98	5	675	2	664	Beta-galactosidase BgaB OS=Carnobacterium maltaromaticum OX=2751 GN=bgaB PE=1 SV=1
D5JGG0	7.65e-97	13	675	11	660	Beta-galactosidase LacZ OS=Weizmannia coagulans OX=1398 GN=lacZ PE=3 SV=1
B9LW38	2.77e-94	10	646	1	644	Beta-galactosidase Bga OS=Halorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34) OX=416348 GN=Hlac_2868 PE=1 SV=1
P94804	3.29e-93	10	644	1	626	Beta-galactosidase BgaH OS=Haloferax lucentense (strain DSM 14919 / JCM 9276 / NCIMB 13854 / Aa 2.2) OX=1230452 GN=bgaH PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000054	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001732_00561.