CAZyme Information: MGYG000001732_00985

Basic Information

• Species: HGM11530 sp900751685
• Lineage: Bacteria; Firmicutes_A; Clostridia; Monoglobales_A; UBA1381; HGM11530; HGM11530 sp900751685
• CAZyme ID: MGYG000001732_00985
• CAZy Family: PL11
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1785		193997.87	4.3445

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001732	3073889	MAG	Sweden	Europe

• Gene Location: Start: 8632; End: 13989 Strand: -

Full Sequence      

MKKLVSFIVSLAMLLTSVSIVIAEGQIQPVTGSEAGGVYTFHIGNTDVSGTGTYNYSDDY
FEVRTAISGNDNITAENGLYYNGGGCAETNSAQDTNRYILIKPVYSGTLNVTINFPSAGA
KSRGCIYYNDFGTTGFDEVDLTTLKKDKGTNFSGNITTSGAKTVRFEAVAGHTYALHTYN
IKSYITALYYELNDPDAPVTSPSPSPAPTPEPTEQPKFEGARQVEYLDRGLVAVKTDGGI
FLSWRLLGTEEVDTAYDIYKNGVLFVKGHDKTNYTDTDGFENDTYKVVVSGEDADGEKAV
NVWSEGHLDVPVSRPVPEPADTKVGTDYTYSANDASVADVDGDGEYEIILKWDPSNSQDN
YFSGYTGNVYVDAYEMDGTMLWRIDMGVNIRAGAHYTQFIAYDFDGDGKAEVAMRTAPGS
KDGKGNYITEAGENLLEYDNTKDYRNAEGRIITAPDYLTIFNGETGEAMKTVDYFVQRGD
LGSSGKWGGSGGDANSNYADRFLAGVAYVNGETPSLIMCRGYYYRAAVGVYDWDGTDLTL
RWSRDDKAEAEDSLYAQGAHSLSISDVDNDGYDEIVYGAAVVDHDGTMLNNTQLKHGDAL
HVSDFNNDGSQEVFMAHEHSYRTHGAEYHGGESGQVFAKVPASEDVGRGVMGNIIASNPL
SEFWSTANNNLYDLNGNVIAKRPAEVNFLAWWDGDLTREVLDATRIVKYDIENGQLVEQY
RKELFGTHSNNGTKQTPSLSADIFGDWREEVMYPTNDNNYLRIYTTDIPTEHKLTTLMHD
TQYRCAVAWQNVGYNQPPHPSFYVGSEKTEYPSPNVKYAAAPEIRPGAQESLYTDIIPKE
SFTGLSDNWGGMVTEEAVPYKQVLSVADGWIKSLSGEESGEYLLEFMWKPLEGSGISVLD
ENGSSIFGLAKSGSVAYFVDDAEKKTISATLHKNSWYCVSIDINTKSQLVSCTIKDYETD
GAQEYSVKDITFAGAQVAQMRSEGANMLDNVHFARVSHDPEHRLLTFDITGGENAAVTVD
GRRVTADNSGTAKITLKDGEYPYTITKSGFKTYNGTAVISGTDKKESAVLLVGEKQNISV
NYKDVFGNTLKDTATAGEAEENESFIVPEEMLADITAADGTVYEFDPDATQSLTANGSNI
DLVYKKKIAPVYGDTDITRINLGENGFHKSAVSADAVEYKKTNGGIKYGLFNGDITAQLN
GRLGGSWYIEYDILLPKLADGDEIKMTPFSDDTAGTPLSFVAENGNVVLKHGSTECTYTS
ETFKKAGYVEGQPLHVTISCTQGKAKLLLFNRENSIVYAGYDLYDAAISDINQLVFSAGE
YNIGISEIEAYTVGGATGASYPFGKAINLTAPSSRFIVPTTVKHGGEYDSKILRFADSNY
FTTEHIDLADGLSYAVTEEDGTAVDAGDVTIDKNTGLITVGADAEKRTVNAHILYNGHVF
KTVPVNIVSGAVESFWTEDFEGGTHKFSLSYNDSAHKVAEFHCEDPSKVNAKAGKIYGVG
ARNGGATGTNSSEIDTSGHSDISVEMDFKLDATSFNGNSYIALLGGQNTADSLGSDKQIL
TVKAFAGGGNGYWSEISVNGKVIITKIDDPNSSGETAGAGYDSSTKTVKFHALNRDSTGW
MHLSAIPDFETQKVNVVISKISSGEIIYSGMLDFVNEVQELKYVFASGGRSYGTAWLDNI
EVKGINPDGSYEPPVREPSTSTDITEENGQYVLASSLNDISSGNLVIAAAYDSENELMSV
KAVDADIKEVETRFDKIDGLSYFKIMVWDGLNKIKPVTKAEYCVL

Enzyme Prediction     

No EC number prediction in MGYG000001732_00985.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL11	222	817	2.7e-229	0.9965694682675815

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd10318	RGL11	0.0	224	802	1	564	Rhamnogalacturonan lyase of the polysaccharide lyase family 11. The rhamnogalacturonan lyase of the polysaccharide lyase family 11 (RGL11) cleaves glycoside bonds in polygalacturonan as well as RG (rhamnogalacturonan) type-I through a beta-elimination reaction. Functionally characterized members of this family, YesW and YesX from Bacillus subtilis, cleave glycoside bonds between rhamnose and galacturonic acid residues in the RG-I region of plant cell wall pectin. YesW and YesX work synergistically, with YesW cleaving the glycoside bond of the RG chain endolytically, and YesX converting the resultant oligosaccharides through an exotype reaction. This domain is sometimes found in architectures with non-catalytic carbohydrate-binding modules (CBMs). There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain through a beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11.
pfam18370	RGI_lyase	3.90e-29	222	303	1	86	Rhamnogalacturonan I lyases beta-sheet domain. This is the beta-sheet domain found in rhamnogalacturonan (RG) lyases, which are responsible for an initial cleavage of the RG type I (RG-I) region of plant cell wall pectin. Polysaccharide lyase family 11 carrying this domain, such as YesW (EC:4.2.2.23) and YesX (EC:4.2.2.24), cleave glycoside bonds between rhamnose and galacturonic acid residues in RG-I through a beta-elimination reaction. Other family members carrying this domain are hemagglutinin A, lysine gingipain (Kgp) and Chitinase C (EC:3.2.1.14).

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QLG37185.1	9.94e-184	220	819	40	622
ASB89979.1	3.53e-183	221	816	40	619
AEP85647.1	7.33e-183	217	816	28	611
QII48617.1	7.08e-182	221	816	40	619
AJW86197.1	7.55e-182	217	816	28	611

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2Z8R_A	1.82e-182	221	816	2	580	Crystalstructure of rhamnogalacturonan lyase YesW at 1.40 A resolution [Bacillus subtilis],2Z8R_B Crystal structure of rhamnogalacturonan lyase YesW at 1.40 A resolution [Bacillus subtilis],2Z8S_A Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid [Bacillus subtilis],2Z8S_B Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid [Bacillus subtilis],2ZUX_A Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose [Bacillus subtilis],2ZUX_B Crystal structure of rhamnogalacturonan lyase YesW complexed with rhamnose [Bacillus subtilis]
4CAG_A	2.22e-181	221	816	8	587	Bacilluslicheniformis Rhamnogalacturonan Lyase PL11 [Bacillus licheniformis]
2ZUY_A	7.97e-175	222	821	6	606	Crystalstructure of exotype rhamnogalacturonan lyase YesX [Bacillus subtilis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O31526	1.36e-181	217	816	34	617	Rhamnogalacturonan endolyase YesW OS=Bacillus subtilis (strain 168) OX=224308 GN=yesW PE=1 SV=1
O31527	3.35e-174	222	821	6	606	Rhamnogalacturonan exolyase YesX OS=Bacillus subtilis (strain 168) OX=224308 GN=yesX PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000248	0.999004	0.000190	0.000189	0.000175	0.000161

TMHMM  Annotations     

start	end
5	27